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Microbial community
Microbiota
great plate count anomaly
16S rRNA gene is a good phylogenetic gene marker because?
hypervariable regions in 16s?
Primers used in microbial ecology studies?
why cant 16s be used for fungi? and how are fungi sequenced?
why can 16s rRNA gene sequence identities be Microbial taxonomic definitions can be blurry/debated?
Approach 1: DNA amplification, cloning and sequencing
Approach 2: DNA “meta-barcoding”
and high-throughput amplicon sequencing
OTU VS ASV
Microbial composition
Alpha vs Beta Diversity
Correlating community compositions with
environmental conditions
Co-occurrence network analyses of taxa between each other
weaknesses
Ribosomal gene operons
