Microscope techniques:

· When transporting , carry with both hand one on the arm of the microscope, one under the base
· Use a lens paper to clean all the lenses and the condenser.
· Raise the condenser to the highest position and open iris diaphragm.
· Turn the microscope lamp on and adjust to maximum intensity.
· Move the scanning (4x) or low power (10x) objective lens into place.
· Place the slide on stage center specimen over opening
· Adjust iris diaphragm to yield optimum illumination
· To focus image. First coarse to oil immersion lens; place drop of immersion oil on specimen. Submerge oil objective lens into oil, use fine focus to view image.
· Objective lenses are par focal
· When storing lower the stage, remove slide; clean lens paper, warp cord around above base of microscope.
· Return to cabinet.

Calculating Magnification:

Total magnification= objective lens magnification ×Ocular lens mag.

Resolution: the eye limits the size of the object visible to the unaided eye.

Immersion Technique:

· Adding oil has the same refractive index as glass, less refracted, more enters lens.

	Created by elsiemm108 almost 11 years ago

Note on Microlab review, created by elsiemm108 on 30/11/2013.

Microlab review