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Year 2 Quiz on Oncogene Classification, created by gina_evans0312 on 02/12/2013.

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Oncogene Classification

Question 1 of 62

1

Give an example of a Class 1 Onocgene- Growth Factors

Select one of the following:

  • Erb-B

  • ERK

  • Sis

Explanation

Question 2 of 62

1

What is the function of the sis protein?

Select one of the following:

  • Forms a PDGF(Beta) chain

  • Forms a IPGF(Beta) chain

  • Forms a IGF1(Beta) chain

Explanation

Question 3 of 62

1

Name the two ways a cell can access extra Growth factor

Select one or more of the following:

  • A virus produces extra growth factor as it replicates

  • A cell begins to make it's own growth factor through growth factor genes

  • A cell begins to steal growth factor from surrounding cells

Explanation

Question 4 of 62

1

Name the Class 2 oncogene

Select one of the following:

  • Growth Factor Receptors

  • Growth Factors

  • Intracellular Transducers

Explanation

Question 5 of 62

1

Name a Class 2 Oncogene

Select one of the following:

  • Erb-B

  • Sis

  • IGF1

  • TGFB

Explanation

Question 6 of 62

1

How does Erb-b become oncogene?

Select one or more of the following:

  • ....bind permanently to their ligand and so become constitutionally active

  • ... no longer need their ligand to activate become constitutionally active

  • ...form a constitutionally active dimer

  • ...no longer needs to dimerise to activate

Explanation

Question 7 of 62

1

What is erb-b?

Select one or more of the following:

  • Family of tyrosine kinase receptors that bind to EGF

  • Family of tyrosine kinase receptors that bind to TGFB

  • Family of tyrosine kinase receptors that bind to IGF1

Explanation

Question 8 of 62

1

Similarly, in 'neu' receptors, what replaces the Valine to make a constantly active receptor?

Select one of the following:

  • Glutamine

  • Glutamic Acid

  • Glutamate

Explanation

Question 9 of 62

1

What is Herceptin?

Select one of the following:

  • A tyrosine kinase agonist?

  • A humanised monoclonal antibody

  • An Erb-B agonist

Explanation

Question 10 of 62

1

What does Herceptin bind to?

Select one of the following:

  • Erb B

  • HER2

  • Tyrosine Kinase

Explanation

Question 11 of 62

1

Name the 3rd Class of Oncogenes

Select one of the following:

  • Growth factors

  • Transcription factors

  • Intracellular transducers

Explanation

Question 12 of 62

1

Which of the following is a class 3A oncogoene?

Select one of the following:

  • Erb-B

  • Src

  • c-myc

Explanation

Question 13 of 62

1

What determines the types of src tyrosine kinase present in the cell?

Select one of the following:

  • The cell type

  • The stage of the cell cycle

  • The age of the cycle

Explanation

Question 14 of 62

1

What is the role of Cellular Transducers

Select one of the following:

  • Relay information to the nucleus to regulate gene transcription

  • Relay information to the ribosomes to regulate protein synthesis

  • Relay information to the Golgi Complex to regulate cell receptor production

Explanation

Question 15 of 62

1

What allows Src to stick to the cytoplasm

Select one of the following:

  • A modified lipid

  • A modified glycoprotein

  • A modified phospholipid

Explanation

Question 16 of 62

1

Name the substance that anchors src to the membrane

Select one of the following:

  • Myristate

  • Palmatite

  • Acetylate

Explanation

Question 17 of 62

1

What holds src kinase shut in its inactive form?

Select one of the following:

  • A phosphate on Tyr 527 in its inactive form & the SH2 domain

  • A phosphate on Tyr 527 in its inactive form & the SH3 domain

  • A phosphate on Tyr 537 in its inactive form & the SH2 domain

Explanation

Question 18 of 62

1

Put the following domains of Src in order
A- Unique Domain
B- Sh2 Domain
C-Catalytic Domain
D- Regulatory Domain
E- SH3

Select one of the following:

  • A-B-E-C-D

  • A-B-C-D-E

  • A-B-D-E-C

Explanation

Question 19 of 62

1

What is the focus of an SH3 domain?

Select one of the following:

  • Protein- protein interaction

  • Protein- phosphotyrosine interaction

  • Protein-phosphoserine interactoin

Explanation

Question 20 of 62

1

What sequence is favored by SH3 domains?

Select one of the following:

  • Proline rich; Pro-X-X-Pro

  • Histadine rich; His-X-X-His

  • Gycine rich; Gy-X-X-Gly

Explanation

Question 21 of 62

1

What is required for Src to activate?

Select one or more of the following:

  • For the protein to be at the membrane

  • For the protein to be cytosolic

  • The SH2 domain then releases the regulatory domain and binds to P-Tyrosine Kinase

Explanation

Question 22 of 62

1

What happens to the regulatory domain once the SH2 domain has released it?

Select one of the following:

  • It's dephosphorylated by a phosphatase at the membrane at Tyr 527

  • It's phosphorylated by the tyrosine kinase at Tyr 527

  • Nothing

Explanation

Question 23 of 62

1

What tyrosine residue is phosphorylated by tyrosine kinase?

Select one of the following:

  • 416 on the activation lip

  • 417 on the activation lip

  • 418 on the activation lip

Explanation

Question 24 of 62

1

What is the most common oncogene form of src?

Select one of the following:

  • Loss of regulatory domain (last 19 amino acids) so it cannot be phosphorylated

  • Loss of SH2 domain so it cannot bind to regulatory domain when phosphorylated

  • Modified lipid binds permanently to the membrane

Explanation

Question 25 of 62

1

Ras is the name of a small family of what?

Select one of the following:

  • GTPase's

  • ATPase's

  • UTPase's

Explanation

Question 26 of 62

1

What process localyses Ras to the membrane?

Select one of the following:

  • Acetylation

  • Methylation

  • Farnesylation

Explanation

Question 27 of 62

1

What is sos?

Select one of the following:

  • A GAP

  • A GEF

  • A GDI

Explanation

Question 28 of 62

1

Where does GTP bind to ras?

Select one of the following:

  • At the C and N terminus

  • In the centre

Explanation

Question 29 of 62

1

What is the purpose of the discrete domain of Ras

Select one of the following:

  • To bind GTP

  • To bind GDP

  • To bind downstream molecules

Explanation

Question 30 of 62

1

What are GDI's?

Select one of the following:

  • Guanine Nucleotide Disassociation Inhibitors

  • Guanine Nucleotide Dimerisation Inhibitors

  • Gunanine Nucleotide Decamerisation Inhibitors

Explanation

Question 31 of 62

1

What is the purpose of a GDI and how it controlled?

Select one or more of the following:

  • By molecules recruited to the tyrosine receptor

  • Bind to Ras and prevent activation

  • Bind to Ras and prevent de-activation

Explanation

Question 32 of 62

1

In which domains can a single mutation turn Ras into an oncogene?

Select one or more of the following:

  • L1

  • L2

  • L3

  • L4

  • L5

Explanation

Question 33 of 62

1

What is the role of GRB2

Select one of the following:

  • Binds Ras to membrane

  • Binds Ras to TK receptor

  • Binds Ras to Raf

Explanation

Question 34 of 62

1

How many SH2 domains does GRB2 have?

Select one of the following:

  • 2- both in the middle

  • 2- one on each end

  • 1 in the middle

  • 1 at the N terminus

  • 1 at the C terminus

Explanation

Question 35 of 62

1

What protein is used by Src to activate Ras?

Select one of the following:

  • Srk

  • Sch

  • Shc

Explanation

Question 36 of 62

1

How does src phosphorylate the protein that activates Ras?

Select one of the following:

  • Binds to SH3 domain

  • Binds to SH2 domain

  • Binds to discrete domain

Explanation

Question 37 of 62

1

Name the two proteins for which phosphorylated Shc has a very high affinity?

Select one or more of the following:

  • Ras

  • Sos

  • GRB2

  • GDI

Explanation

Question 38 of 62

1

Name the 4th class of oncogenes

Select one of the following:

  • Growth factors

  • Cellular Transporters

  • Growth Factor Receptors

  • Transcription factors

Explanation

Question 39 of 62

1

Name a class 4 oncogene

Select one of the following:

  • Src

  • Ras

  • Jun

Explanation

Question 40 of 62

1

Name the part of c-jun that causes dimerisation

Select one of the following:

  • Leu Zipper

  • His zipper

  • Ala zipper

Explanation

Question 41 of 62

1

Where is the unique sequence of c-jun and what is it's role?

Select one or more of the following:

  • Near the center

  • Near the c-terminus

  • Near the n-terminus

  • Defines family member

  • Determines where in the DNA c-jun binds

Explanation

Question 42 of 62

1

Put the domains of C-jun in the correct order A-Leu Zipper B- Delta Domain C-Transcription Domain D- Unique Domain E- DNA Binding Domain

Select one of the following:

  • D-B-C-E-A

  • D-E-A-B-C

  • C-A-D-B-E

Explanation

Question 43 of 62

1

How does C-jun function

Select one of the following:

  • As a homodimer

  • As a heterodimer

  • As both

Explanation

Question 44 of 62

1

What is the first step of c-jun homodimer activation?

Select one of the following:

  • Dephosphorylation by PKC

  • Phosphorylation by JNK (ERK parallel protein)

Explanation

Question 45 of 62

1

What is the second step of c-jun homodimer activation?

Select one of the following:

  • Dephosphorylation by PKB

  • Phosphorylation by JNK

Explanation

Question 46 of 62

1

What part(s) of c-jun is lost to make it an oncogene?

Select one or more of the following:

  • Unique sequence

  • Delta Domain

  • Leu zipper

Explanation

Question 47 of 62

1

Name a protein likely to form a heterodimer with c-jun

Select one of the following:

  • AFK

  • AFT

  • AFR

Explanation

Question 48 of 62

1

What tends to be transcribed by the first c-jun heterodimer to form?

Select one of the following:

  • c-jun

  • AFK

  • Fos

Explanation

Question 49 of 62

1

What is the second protein to heterodimerise with c-jun?

Select one of the following:

  • Fos

  • Fas

  • Fis

Explanation

Question 50 of 62

1

What is the name of the second heterodimer formed by c-jun?

Select one of the following:

  • AP1

  • Ap2

  • AP

Explanation

Question 51 of 62

1

What is required for Fos transcription?

Select one of the following:

  • TCF/AFK to be activated by ERK

  • AFK to be activated by ERK

  • TCF to be activated by ERK

Explanation

Question 52 of 62

1

What sequences does AP1 bind to?

Select one or more of the following:

  • TGAC

  • GTAC

  • GGAC

Explanation

Question 53 of 62

1

What is transcribed by AP1?

Select one of the following:

  • Transcription factors

  • Ribosomal proteins

  • Growth factors

Explanation

Question 54 of 62

1

Is Cyclin D1 transcribed by Jun?

Select one of the following:

  • Yes

  • No

Explanation

Question 55 of 62

1

What happens if the signal activating AP1 persists for 30 mins?

Select one or more of the following:

  • It's activity is increased further by phosphorylation

  • It's activity is increased further by methylation

  • By Jnk/Erk

Explanation

Question 56 of 62

1

What transcribes C-Myc?

Select one of the following:

  • API

  • Fos

  • c-Jun

Explanation

Question 57 of 62

1

How long is the half life of C-myc?

Select one of the following:

  • 5-10 mins

  • 5-10 seconds

  • 3-5 mins

Explanation

Question 58 of 62

1

What does Myc bind to in order to interact with E-Box sequences?

Select one of the following:

  • Max

  • Mad

  • Mac

Explanation

Question 59 of 62

1

Why is Ubiquitin ligase transcribed by c-myc?

Select one of the following:

  • Halts protein turnover & allows protein levels to build up

  • Increases protein turnover so only new proteins are involved in the cell cycle

Explanation

Question 60 of 62

1

In normal cells, which of the following is in excess, and why?

Select one of the following:

  • Max, so the dimer only forms when Myc levels are signaled to rise

  • Mic, so the dimer only forms when Max levels are signalled to rise

Explanation

Question 61 of 62

1

How are Max homodimers inhibitory?

Select one of the following:

  • They block jun transcription sites

  • They block jun expression promoters

  • They block myc expression promoters

Explanation

Question 62 of 62

1

What is different in tumor cells from normal cells (concerning myc/max levels)?

Select one of the following:

  • Myc levels are always higher than Max

  • Max levels are always higher than myc

Explanation

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