Pip Fraggle
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Microbiology Quiz on Enterobeacteriaceae, created by Pip Fraggle on 19/09/2016.

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Enterobeacteriaceae

Question 1 of 23

1

How are Enterobacteraceae classified according to a gram stain?

Select one of the following:

  • Gram negative bacilli

  • Gram negative coccobacilli

  • neither

  • both

Explanation

Question 2 of 23

1

All Enterobacteriaceae ferment glucose

Select one of the following:

  • True
  • False

Explanation

Question 3 of 23

1

Not all members of the Enterobacteriaceae family are cytochrom-oxidase negative.

Select one of the following:

  • True
  • False

Explanation

Question 4 of 23

1

Enterobacteriaceae are obligatory anaerobes

Select one of the following:

  • True
  • False

Explanation

Question 5 of 23

3

Select from the dropdown lists to complete the text.

Match the Classification with the antigen

Somatic ( O, K, H )
Envelope ( O, K, H )
Flagellar ( O, K, H )

Explanation

Question 6 of 23

3

Select from the dropdown lists to complete the text.

Match the Antigen with its description.

Endotoxin, heat stable part of the outer cell wall ( O, K, H )
Heat Labile capsular polysaccharide surrounding the cell wall, covers the O antigen ( O, K, H )
Heat labile protein found in the flagellum of mobile Enterobacteriaceae ( O, K, H )

Explanation

Question 7 of 23

2

Which Media are usually used for the isolation of Enterobacteriaceae?

Select one or more of the following:

  • Trypticase Soy Agar

  • Blood Agar

  • Chocolate Agar

  • MacConkey Agar/Eosin-Methylene Blue (EMB) agar

  • Thiglycolatte Broth

  • Hektoen Enteric(HE)/Salmonella-Shigella (SS)/Xylose-lysine-deoxycholate (XLD) agar

  • Colistin-Nalidixic Acd (CNA) agar

  • Martin Lewis medium

Explanation

Question 8 of 23

1

What reaction would one expect to see on a blood agar if the organism was positive for Hydrogen Sulfide?

Select one of the following:

  • subsurface greening

  • subsurface clearing

  • subsurface yellowing

  • subsurface browning

Explanation

Question 9 of 23

4

Select from the dropdown lists to complete the text.

Match the IMViC test with the importance of the result it generates

E. coli is always positive: If the bacteria is positive in this test it means that the bacteria contains tryptophanase to convert tryptophan to ammonia and indole ( Indole, Methyl Red, Voges-Prokauer, Citrate )
E. Coli is always positive: If the bacteria is positive in this test it means it is able to convert glucose to pyruvic acid ( Indole, Methyl Red, Voges-Prokauer, Citrate )
E. Coli is negative/Klebsiella is Positive: If the bacteria is positive in this test it means that it is able to convert glucose to acetoin ( Indole, Methyl Red, Voges-Prokauer, Citrate )
E. Coli is negative/Kelbsiella is positive. If the bacteria is positive in this test it means that it uses citrate as its main source of carbon ( Indole, Methyl Red, Voges-Prokauer, Citrate )

Explanation

Question 10 of 23

3

Select the sugars present in Triple Sugar Iron (TSI) agar

Select one or more of the following:

  • glucose

  • lactose

  • sucrose

  • mannose

  • sorbitol

  • mannitol

  • trehalose

Explanation

Question 11 of 23

1

Which test is used to look for slow/late fermenters?

Select one of the following:

  • ONPG reaction

  • SIM

  • TSI

  • MIO

Explanation

Question 12 of 23

1

Which test is used to determine if an organism can convert urea into ammonia, carbon dioxide and water?

Select one of the following:

  • Urease

  • MIO

  • ONPG reaction

  • Deaminase

Explanation

Question 13 of 23

1

What colour will a Urease test change to if the organism is Urease positive?

Select one of the following:

  • hot pink

  • neon green

  • brown

  • Yellow

Explanation

Question 14 of 23

2

Select from the dropdown lists to complete the text.

Match the urea producer will its speed of production

Proetus ( fast producer, slow producer )
Klebsiella ( slow producer, fast producer )

Explanation

Question 15 of 23

2

Which two organisms will test negative in a motility test?

Select one or more of the following:

  • Shigella

  • Klebsiella

  • Salmonella

  • Enterobacter

  • Serratia

  • Hafnia

  • Proteus

  • Citrobacter

  • Morganella

  • Providencia

Explanation

Question 16 of 23

3

Which organisms will test positive for a Deaminase/Phenylalanine Deaminsae (PAD) test?

Select one or more of the following:

  • Proteus

  • Morganella

  • Providencia

  • Shigella

  • Salmonella

  • Klebsiella

  • Enterobacter

  • Serratia

  • Hafnia

  • Citrobacter

Explanation

Question 17 of 23

3

Match the test colour result with its corresponding conclusion.

Acid Production
No Acid Production
Base production due to use of peptone by organism

Drag and drop to complete the text.

    Yellow
    Red
    Pink

Explanation

Question 18 of 23

1

Which test is a good choice when one wants to distinguish Enterobacteriaceae from gram negative rods that do NOT ferment glucose?

Select one of the following:

  • Triple Iron Sugar (TIS)

  • SIM agar

  • Motility Test

Explanation

Question 19 of 23

5

Match the colour result from a Triple Sugar Iron (TSI) test with the correct conclusion.

Bacteria ferments glucose but not sucrose or lactose. Alkaline slant over acid deep (K/A).
Bacteria ferments glucose, lactose and/or sucrose. Acid slant over Acid deep (A/A).
Bacteria is not able to ferment glucose. Alkaline Slant over Alkaline deep (K/K)
Production of gas
Bacteria produces Hydrogen Sulfide (H2s)

Drag and drop to complete the text.

    Red over Yellow
    Yellow over Yellow
    Red over Red
    Cracks or medium pulling away
    Black precipitate

Explanation

Question 20 of 23

1

Which Species of Enterobacteriaceae will produce an acid slant and a deep acid (A/A) result with Gas production and no production of Hydrogen Sulfide in a Triple Sugar Iron (TSI) test.

Select one of the following:

  • E. Coli

  • Salmonella typhimurium

  • Shigella Flexneri

  • Pseudomonas aeruginiosa

Explanation

Question 21 of 23

1

Which species of Enterobacteriaceae will produce an Alkaline slant over a deep acid with positive gas production and positive hydrogen sulfide production in a Triple Sugar Iron (TSI) test?

Select one of the following:

  • Escherichia coli

  • Salmonella typhimurium

  • Shigella flexneri

  • Pseudomonas aeruginosa

Explanation

Question 22 of 23

1

Which species of Enterobacteriaceae will produce an Alkaline slant over a deep acid with negative gas production and negative hyrdogen sulfide production in a Triple Sugar Iron (TSI) test?

Select one of the following:

  • Escherichia coli

  • Salmonella typhimurium

  • Shigella flexneri

  • Pseudomonas aeruginosa

Explanation

Question 23 of 23

1

Which species of Enterobacteriaceae will produce an Alkaline slant over a deep alkalin with negative gas production and negative hyrdogen sulfide production in a Triple Sugar Iron (TSI) test?

Select one of the following:

  • Escherichia coli

  • Salmonella typhimurium

  • Shigella flexneri

  • Pseudomonas aeruginosa

Explanation

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