Genetics

Question 1 of 32

1

Of the following polymorphisms which are single nucleotide differences that are seen between two genomes?

Select one of the following:

Restriction fragment length polymorphisms
Short tandem repeats
Variable number tandem repeats
Single nucleotide polymorphisms
Chromosomal translocation

Explanation

Question 2 of 32

1

Which of the following polymorphisms can be detected through Southern blot?

Select one of the following:

Short tandem repeats
Single nucleotide polymorphisms
Restriction fragment length polymorphisms
Long interspersed nucleotide sequences

Explanation

Question 3 of 32

1

What does it mean, that a locus is homozygous?

Select one of the following:

many different alleles
two alleles that are the same
two alleles that are different
no alleles
one allele have been deleted

Explanation

Question 4 of 32

1

The following is a description of which method.
Patients DNA was isolated and cut with HaeIII. Product of the restriction digest was resolved by gel electrophoresis and transferred to a membrane. Then the membrane was exposed to hybridization probes, and the resulting band pattern was compared with that seen in another sample of DNA.

Select one of the following:

RFLP by Southern blotting
VNTR by PCR
SNP by sequencing
STR by PCR
SNP by Western Blot

Explanation

Question 5 of 32

1

FGA, D8S1179, and D2S11 are examples of which of the following?

Select one of the following:

RFLP
VNTR
STR
SNP

Explanation

Question 6 of 32

1

Which of the following is used as a marker of gender?

Select one of the following:

D8S1179
Amelogenin
TPOX
PentaD
CD4

Explanation

Question 7 of 32

1

How many loci are considered core loci by the Federal Bureau of Investigation in the Combined DNA Indexing System?

Select one of the following:

10
12
13
16
20

Explanation

Question 8 of 32

1

When two sources of DNA are compared by STR analysis in a crime scene, how many differences between alleles will determine that the two sources are different?

Select one of the following:

1
2
4
6
25

Explanation

Question 9 of 32

1

Which of the following polymorphisms can be used to compare DNA samples derived from males but not females?

Select one of the following:

STR
Y-STR
RFLP
VNTR
SNP

Explanation

Question 10 of 32

1

Which of the following STRs is a Y-STR?

Select one of the following:

D5S818
vWA
Penta E
DYS385
LPL

Explanation

Question 11 of 32

1

A child has disputed paternity. The child has the following STR haplotype:
TPOX = 7/8; D5S818 = 11/12; D13S317 = 9/10; vWA = 11/12; D21S11 = 27/28.
Alleged Father 1 has the following STR haplotype: TPOX = 6/10; D5S818 = 9/13; D13S317 = 9/14; vWA = 14/16; D21S11 = 30/33.
What can be said of Alleged Father 1?

Select one of the following:

He is the father of the child
He cannot be excluded as the father
He is excluded as the father.
More STRs need to be analyzed before a conclusion can be made.

Explanation

Question 12 of 32

1

An individual who has two populations of cells that were derived from two different zygotes is called a:

Select one of the following:

chimera
mosaic
heteroplasmy
mutant

Explanation

Question 13 of 32

1

A woman has been raped by an unknown man. Which of the following laboratory procedures can be used to more accurately analyze residual DNA left by the man in an effort to identify him?

Select one of the following:

RFLP
STR
Y-STR
VNTR

Explanation

Question 14 of 32

1

DNA found at the scene of the crime was determined to have one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the following?

Select one of the following:

Female
Male
Impossible to tell from one locus
This locus does not discriminate between males and females

Explanation

Question 15 of 32

1

STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons?

Select one of the following:

To determine whether the donor and recipient are a match
To determine the likelihood of graft-versus-host disease
To find informative alleles for post-transplantation studies
To add their STR haplotypes to a national database

Explanation

Question 16 of 32

1

STR analysis was performed on a bone marrow donor and recipient pair with the following results. Which loci are informative?

Select one of the following:

vWA and TH01
TH01 and D13S317
D16S539 and F13A01
vWA and D13S317

Explanation

Question 17 of 32

1

Bone marrow has been transplanted into a recipient. STR analysis was performed on whole blood from the recipient 6 months after the transplant by PCR, followed by capillary gel electrophoresis with fluorescence measurement of amplicons. For the TPOX locus, the donor and recipient do not share alleles. The fluorescence of the TPOX locus for the donor peak was 15693, and the fluorescence of the TPOX locus for the recipient peak was 5329. What is the percentage of recipient cells remaining?

Select one of the following:

25%
34%
66%
75%

Explanation

Question 18 of 32

1

Two people have what percentage of DNA sequence that differs between them?

Select one of the following:

1%
5%
10%
25%
15%

Explanation

Question 19 of 32

1

Two middle-age women who had been adopted into different families as infants discover circumstantially that they may have the same biological mother. Which of the following analyses would give the best information toward proving their maternal lineage?

Select one of the following:

Y-STR
Mitochondrial DNA
RFLP-PCR
SNP haplotypes

Explanation

Question 20 of 32

1

Consider the following STR analysis:
Which of the child alleles are inherited form the father?

Select one of the following:

D3S1358: 15, vWA: 18, FGA: 24, TH01: 10 , TPOX: 11, CSF1PO: 12, D5S818: 12, D13S317: 9
D3S1358: 4, vWA: 5, FGA: 20, TH01: 11 , TPOX: 10, CSF1PO: 13, D5S818: 3, D13S317: 6
D3S1358: 15, vWA: 17, FGA: 23, TH01: 6 , TPOX: 11, CSF1PO: 12, D5S818: 10, D13S317: 10
D3S1358: 10, vWA: 17, FGA: 24, TH01: 6 , TPOX: 10, CSF1PO: 12, D5S818: 10, D13S317: 9
D3S1358: 15, vWA: 18, FGA: 24, TH01: 10 , TPOX: 11, CSF1PO: 12, D5S818: 12, D13S317: 10

Explanation

Question 21 of 32

1

Basked on the results which of the alleged fathers (AF) is the father?

Select one of the following:

AF1
AF2
Neither

Explanation

Question 22 of 32

1

A child and an alleged father (AF) share alleles with the following paternity index:
What is the combined paternity index from these three loci?

Select one of the following:

4.131
4.5
5
3.131

Explanation

Question 23 of 32

1

With 50% prior odds, what is the probability of paternity from these three loci?

Select one of the following:

80%
76%
90%
60%

Explanation

Question 24 of 32

1

What is a traditional definition of a polymorphism?

Select one of the following:

The genets variation must be present in at least 1 % of the population.
The genets variation must be present in at least 5 % of the population.
The genets variation that no longer is present in the population.
The genets variation can not be present in majority of the population.

Explanation

Question 25 of 32

1

Which of the following genomic variations is the hardest to detect?

Select one of the following:

SNP
STRs
VNTRs
RFLPs

Explanation

Question 26 of 32

1

When comparing a generic similarity go two random individuals, what percentage of their genome is identical?

Select one of the following:

99.5%
95%
90%
100%
80%

Explanation

Question 27 of 32

1

RFLP stands for?

Select one of the following:

Restriction fragment length polymorphism
Rapidly Fusing length polymorphisms
Rescue factor logical polymorphisms
Redundant fragment length polymorphism

Explanation

Question 28 of 32

1

Closely linked markers are used for:

Select one of the following:

Accurate mapping of regions associated with phenotypic characteristics
Finding the length of a sequence
Location of the centromere
Defining the promoter
Disabling the binding of transcription factors

Explanation

Question 29 of 32

1

What is a definition of a haplotype?

Select one of the following:

A block of closely linked SPS on the same chromosome that tend to be inherited together.
A half of a chromosome.
Chromosome with only one functioning allele.
Allele that gives a disfiguring phenotype.

Explanation

Question 30 of 32

1

Mitochondrial DNA is____

Select one of the following:

circular double stranded
circular single stranded
linear double stranded
linear single stranded

Explanation

Question 31 of 32

1

Genes encoded on the mtDNA include:

Select one of the following:

22 tRNA genes, 2 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
30 tRNA genes, 2 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
22 tRNA genes, 6 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
22 tRNA genes, 2 ribosomal RNA genes, and 60 genes coding for components of the oxidation-phosphorylation system.

	Created by mona1nyc over 10 years ago

DNA Polymorphisms and Human Identification MB ASCP

Question 1 of 32

Of the following polymorphisms which are single nucleotide differences that are seen between two genomes?

Select one of the following:

Explanation

Question 2 of 32

Which of the following polymorphisms can be detected through Southern blot?

Select one of the following:

Explanation

Question 3 of 32

What does it mean, that a locus is homozygous?

Select one of the following:

Explanation

Question 4 of 32

Select one of the following:

Explanation

Question 5 of 32

FGA, D8S1179, and D2S11 are examples of which of the following?

Select one of the following:

Explanation

Question 6 of 32

Which of the following is used as a marker of gender?

Select one of the following:

Explanation

Question 7 of 32

How many loci are considered core loci by the Federal Bureau of Investigation in the Combined DNA Indexing System?

Select one of the following:

Explanation

Question 8 of 32

When two sources of DNA are compared by STR analysis in a crime scene, how many differences between alleles will determine that the two sources are different?

Select one of the following:

Explanation

Question 9 of 32

Which of the following polymorphisms can be used to compare DNA samples derived from males but not females?

Select one of the following:

Explanation

Question 10 of 32

Which of the following STRs is a Y-STR?

Select one of the following:

Explanation

Question 11 of 32

Select one of the following:

Explanation

Question 12 of 32

An individual who has two populations of cells that were derived from two different zygotes is called a:

Select one of the following:

Explanation

Question 13 of 32

A woman has been raped by an unknown man. Which of the following laboratory procedures can be used to more accurately analyze residual DNA left by the man in an effort to identify him?

Select one of the following:

Explanation

Question 14 of 32

DNA found at the scene of the crime was determined to have one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the following?

Select one of the following:

Explanation

Question 15 of 32

STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons?

Select one of the following:

Explanation

Question 16 of 32

STR analysis was performed on a bone marrow donor and recipient pair with the following results. Which loci are informative?

Select one of the following:

Explanation

Question 17 of 32

Select one of the following:

Explanation

Question 18 of 32

Two people have what percentage of DNA sequence that differs between them?

Select one of the following:

Explanation

Question 19 of 32

Two middle-age women who had been adopted into different families as infants discover circumstantially that they may have the same biological mother. Which of the following analyses would give the best information toward proving their maternal lineage?

Select one of the following:

Explanation

Question 20 of 32

Consider the following STR analysis: Which of the child alleles are inherited form the father?

Select one of the following:

Explanation

Question 21 of 32

Consider the following STR analysis:
Which of the child alleles are inherited form the father?

A child and an alleged father (AF) share alleles with the following paternity index:
What is the combined paternity index from these three loci?