Eva Bonning
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Medicine (CELS 191) Quiz on CELS 191 Lecture 16, created by Eva Bonning on 24/05/2017.

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Eva Bonning
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CELS 191 Lecture 16

Question 1 of 20

1

Select the statements that apply to prokaryotic DNA

Select one or more of the following:

  • Thy have a single, circular chromosome

  • There is a single point of origin for DNA replication

  • DNA replication is bidirectional

  • They have lots of linear DNA

  • There are multiple points of origin

Explanation

Question 2 of 20

1

Select the statements that apply to eukaryotic DNA

Select one or more of the following:

  • They have lots of linear DNA

  • There are multiple points of origin for DNA replication

  • DNA replication is bidirectional

  • There is one, circular chromosome

  • There is one point of origin for DNA replication

Explanation

Question 3 of 20

1

What is the role of the primase enzyme in DNA replication

Select one of the following:

  • It adds RNA primers to the template strand

  • It unzips the DNA strand

  • It releases the tension at the DNA fork

  • It join synthesised fragments with phosphodiester bonds

Explanation

Question 4 of 20

1

What is the role of the DNA Polymerase III enzyme in DNA replication

Select one of the following:

  • It adds complimentary nucleotides from the reactive 3' hydroxyl group left by the RNA primer

  • It adds complimentary base pairs from the reactive 3' phosphate group left by the RNA primer

  • It binds at the 5' end and synthesises the new strand by adding base pairs

  • It binds at the 3' end and synthesises the new strand by adding phosphodiester bonds

Explanation

Question 5 of 20

1

What is the role of the Helicase enzyme in DNA replication

Select one of the following:

  • It unwinds the DNA from the AT rich point of origin

  • It unwinds the DNA from the point of origin

  • It unwinds the DNA from the CG rich point of origin

  • It puts the DNA back together after synthesis

Explanation

Question 6 of 20

1

What is the role of the Topoisomerase enzyme in DNA replication

Select one of the following:

  • It releases the tension at the replication fork by making cuts and then 'glues' them back together

  • It binds to the DNA to stop the leading and lagging strand fro getting back together

  • It unzips the gene

  • It checks the newly added bases against the template to make sure they are correct

Explanation

Question 7 of 20

1

What is the role of single stranded binding proteins in DNA replication

Select one or more of the following:

  • They protect the single strands from degradation

  • They prevent the single strands from coming back together again

  • They make RNA primers

  • The release the tension at the replication fork by making cuts and then gluing them back together again

Explanation

Question 8 of 20

1

What is the role of Ligase in DNA replication

Select one of the following:

  • It joins synthesised fragments with phosphodiester bonds

  • It unzips the gene

  • It releases the tension at the replication fork

  • It makes RNA primers

Explanation

Question 9 of 20

1

The overall direction of DNA synthesis on the lagging strand is in the 3'-5' direction

Select one of the following:

  • True
  • False

Explanation

Question 10 of 20

1

How are errors in DNA sequence corrected during replication?

Select one of the following:

  • With exonuclease activity of DNA Polymerase III

  • With endonuclease activity of DNA polymerase III

  • With exonuclease activity of DNA Polymerase II

  • With endonuclease activity of DNA polymerase II

Explanation

Question 11 of 20

1

DNA replication, errors in DNA sequence are corrected with activity of DNA polymerase . It checks the newly added bases against the , removes it and replaces it so synthesis can continue as normal.

Drag and drop to complete the text.

    exonuclease
    endonuclease
    III
    II
    template
    lagging strand
    During
    After

Explanation

Question 12 of 20

1

DNA replication, any errors in the sequence are corrected by activity of DNA polymerase . it removes the incorrect bases and some either side, corrects the mistake, then the newly synthesised sequence to the pre-existing sequence with .

Drag and drop to complete the text.

    After
    During
    endonuclease
    exonuclease
    I
    III
    DNA polymerase
    Ligase
    joins
    detaches
    phosphodiester bonds
    hydrogen bonds

Explanation

Question 13 of 20

1

Fill the blank spaces to complete the text.

Failure to mistakes in DNA sequence will lead to changes in sequence, in turn leading to a

Explanation

Question 14 of 20

1

Fill the blank spaces to complete the text.

PCR (P C R) a gene so you can have copies of the gene. There are no strands (unlike prokaryotic replication) as both strands are synthesised .

Explanation

Question 15 of 20

1

Select the key components of PCR

Select one or more of the following:

  • DNA template

  • Primers

  • Heat stable DNA polymerase

  • dNTPS

  • Buffer solution

  • Divalent Cations

  • Covalent Anions

  • RNA template

  • ddNTPS

  • DNA polymerase

Explanation

Question 16 of 20

1

At what temperature does Denaturation of double stranded DNA occur during PCR?

Select one of the following:

  • 95C

  • 45-70C

  • 72C

  • 85C

Explanation

Question 17 of 20

1

At what temperature does Annealing of DNA templates occur during PCR?

Select one of the following:

  • 95C

  • 45-70C

  • 72C

  • 105C

Explanation

Question 18 of 20

1

At what temperature does extension/elongation occur during PCR?

Select one of the following:

  • 72C

  • 95C

  • 50C

  • 40-75C

Explanation

Question 19 of 20

1

What is the role of DNA polymerase I during DNA replication

Select one or more of the following:

  • It removes the RNA primers on Okazaki fragments

  • It fills in the gaps left by removed RNA primers with complimentary nucleotides

  • It adds the RNA primers on Okazaki fragments

  • It fills in the gaps left by removed DNA primers with RNA

Explanation

Question 20 of 20

1

Fill the blank spaces to complete the text.

DNA replication begins with unwinding the DNA strands from an rich origin of replication, with (SSBP) protecting the strands from and stopping them from coming back together again. Topoisomerase releases at the replication fork by cutting and gluing the strands back together again. adds an primer with a chemically reactive ’ hydroxyl group, then DNA Polymerase adds complimentary from that hydroxyl group. On the leading strand, synthesis happens in the direction, however on the lagging strand this occurs in the overall direction in fragments. RNA primers are added intermittently along the strand, with DNA Polymerase III adding the complementary nucleotides in the 5’-3’ direction. DNA Polymerase then comes and removes the RNA primers on the leading and lagging strands, and fills in the gaps with nucleotides. forms the bonds between 3’ hydroxyl and 5’ phosphate groups, completing DNA synthesis.

Explanation

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