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Microbiology Test

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This test will aid medical technology students to show how much they have learned from the subject matter.
Darla Delos Reyes
Quiz by Darla Delos Reyes, updated more than 1 year ago
Darla Delos Reyes
Created by Darla Delos Reyes almost 7 years ago
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Resource summary

Question 1

Question
The aseptic collection of blood cultures requires that the skin be cleansed with:
Answer
  • A. 2% iodine and then 70% alcohol solution
  • B. 70% alcohol and then 2% iodine or an iodophor
  • C. 70% alcohol and then 95% alcohol
  • D. 95% alcohol only

Question 2

Question
When cleansing the skin with alcohol and then iodine for the collection of a blood culture, the iodine (or iodophor) should remain intact on the skin for at least:
Answer
  • A. 10 sec
  • B. 30 sec
  • C. 60 sec
  • D. 5 min

Question 3

Question
What is the purpose of adding 0.025%–0.050% sodium polyanetholsulfonate (SPS) to nutrient broth media for the collection of blood cultures?
Answer
  • A. It inhibits phagocytosis and complement
  • B. It promotes formation of a blood clot
  • C. It enhances growth of anaerobes
  • D. It functions as a preservative

Question 4

Question
A flexible calcium alginate nasopharyngeal swab is the collection device of choice for recovery of which organism from the nasopharynx?
Answer
  • A. Staphylococcus aureus
  • B. Streptococcus pneumoniae
  • C. Corynebacterium diphtheriae
  • D. Bacteroides fragilis

Question 5

Question
Semisolid transport media such as Amies, Stuart, or Cary–Blair are suitable for the transport of swabs for culture of most pathogens except:
Answer
  • A. Neisseria gonorrhoeae
  • B. Enterobacteriaceae
  • C. Campylobacter fetus
  • D. Streptococcus pneumoniae

Question 6

Question
Select the method of choice for recovery of anaerobic bacteria from a deep abscess.
Answer
  • A. Cotton fiber swab of the abscess area
  • B. Skin snip of the surface tissue
  • C. Needle aspirate after surface decontamination
  • D. Swab of the scalpel used for débridement

Question 7

Question
7. Select the primary and differential media of choice for recovery of most fecal pathogens.
Answer
  • A. MacConkey, blood, birdseed, and Campylobacter (Campy) agars
  • B. Hektoen, MacConkey, Campy, colistin–nalidixic acid (CNA) agars
  • C. CNA and Christensen urea agars and thioglycollate media
  • D. Blood, Campy, Mueller–Hinton agars, and thioglycollate media

Question 8

Question
8. Select the media of choice for recovery of Vibrio cholerae from a stool specimen.
Answer
  • A. MacConkey agar and thioglycollate media
  • B. Thiosulfate–citrate–bile–sucrose (TCBS) agar and alkaline peptone water (APW) broth
  • C. Blood agar and selenite-F (SEL) broth
  • D. CNA agar

Question 9

Question
Colistin–nalidixic acid agar (CNA) is used primarily for the recovery of:
Answer
  • A. Neisseria species
  • B. Enterobacteriaceae
  • C. Pseudomonas aeruginosa
  • D. Staphylococcus aureus

Question 10

Question
10. In the United States, most blood agar plates are prepared with 5% or 10% red blood cells (RBCs) obtained from:
Answer
  • A. Sheep
  • B. Horses
  • C. Humans
  • D. Dogs

Question 11

Question
All of the following are appropriate when attempting to isolate N. gonorrhoeae from a genital specimen except:
Answer
  • A. Transport the genital swab in charcoal transport medium
  • B. Plate the specimen on modified Thayer–Martin (MTM) medium
  • C. Plate the specimen on New York City or Martin–Lewis agar
  • D. Culture specimens in ambient oxygen at 37°C

Question 12

Question
Chocolate agar and modified Thayer–Martin agar are used for the recovery of:
Answer
  • A. Haemophilus spp. and Neisseria spp., respectively
  • B. Haemophilus spp. and N. gonorrhoeae, respectively
  • C. Neisseria spp. and Streptococcus spp., respectively
  • D. Streptococcus spp. and Staphylococcus spp., respectively

Question 13

Question
Cycloserine–cefoxitin-fructose agar (CCFA) is used for the recovery of:
Answer
  • A. Yersinia enterocolitica
  • B. Yersinia intermedia
  • C. Clostridium perfringens
  • D. Clostridium difficile

Question 14

Question
Deoxycholate agar (DCA) is useful for the isolation of:
Answer
  • A. Enterobacteriaceae
  • B. Enterococcus spp.
  • C. Staphylococcus spp.
  • D. Neisseria spp.

Question 15

Question
Xylose lysine deoxycholate (XLD) agar is a highly selective medium used for the recovery of which bacteria?
Answer
  • A. Staphylococcus spp. from normal flora
  • B. Yersinia spp. that do not grow on Hektoen agar
  • C. Enterobacteriaceae from gastrointestinal specimens
  • D. Streptococcus spp. from stool cultures

Question 16

Question
A sheep blood agar plate is used as a primary isolation medium when all of the following organisms are to be recovered from a wound specimen except:
Answer
  • A. β-Hemolytic streptococci and coagulase-positive staphylococci
  • B. Haemophilus influenzae and Haemophilus parainfluenzae
  • C. Proteus spp. and Escherichia coli
  • D. Pseudomonas spp. and Acinetobacter spp.

Question 17

Question
Prereduced and vitamin K1-supplemented blood agar plates are recommended isolation media for:
Answer
  • A. Mycobacterium marinum and Mycobacterium avium intracellulare
  • B. Bacteroides, Peptostreptococcus, and Clostridium spp.
  • C. Proteus spp.
  • D. Enterococcus spp.

Question 18

Question
Which procedure is appropriate for culture of genital specimens in order to recover Chlamydia spp.?
Answer
  • A. Inoculate cycloheximide-treated McCoy cells
  • B. Plate onto blood and chocolate agar
  • C. Inoculate into thioglycollate (THIO) broth
  • D. Plate onto modified Thayer–Martin agar within 24 hours

Question 19

Question
Specimens for virus culture should be transported in media containing:
Answer
  • A. Antibiotics and 5% sheep blood
  • B. Saline and 5% sheep blood
  • C. 22% bovine albumin
  • D. Antibiotics and nutrient

Question 20

Question
Cerebrospinal fluid (CSF) should be cultured immediately, but if delayed the specimen should be:
Answer
  • A. Refrigerated at 4°C to 6°C
  • B. Frozen at –20°C
  • C. Stored at room temperature for no longer than 24 hours
  • D. Incubated at 37°C and cultured as soon as possible

Question 21

Question
The most sensitive method for the detection of β-lactamase in bacteria is by the use of:
Answer
  • A. Chromogenic cephalosporin
  • B. Penicillin
  • C. Oxidase
  • D. Chloramphenicol acetyltransferase

Question 22

Question
The breakpoint of an antimicrobial drug refers to:
Answer
  • A. The amount needed to cause bacteriostasis
  • B. A minimum inhibitory concentration (MIC) of 16 μg/mL or greater
  • C. A MIC of 64 μg/mL or greater
  • D. The level of drug that is achievable in serum

Question 23

Question
Which of the following variables may change the results of an MIC?
Answer
  • A. Inoculum size
  • B. Incubation time
  • C. Growth rate of the bacteria
  • D. All of these options

Question 24

Question
According to the Kirby–Bauer standard antimicrobial susceptibility testing method, what should be done when interpreting the zone size of a motile, swarming organism such as a Proteus species?
Answer
  • A. The swarming area should be ignored
  • B. The results of the disk diffusion method are invalid
  • C. The swarming area should be measured as the growth boundary
  • D. The isolate should be retested after diluting to a 0.05 McFarland standard

Question 25

Question
Which class of antibiotics is used for the treatment of serious gram-negative infections as well as infections with Mycobacterium tuberculosis?
Answer
  • A. Cephalosporins
  • B. Penicillins
  • C. Tetracyclines
  • D. Aminoglycosides

Question 26

Question
Select the medium best suited for the recovery of Yersinia enterocolitica from a patient with gastroenteritis.
Answer
  • A. Hektoen agar
  • B. Cefsulodin–Irgasan–Novobiocin (CIN) agar
  • C. Blood agar
  • D. Eosinmethylene blue agar

Question 27

Question
A suspected case of plague requires which of the following procedures in order to confirm Yersinia pestis?
Answer
  • A. Collection of multiple sets of blood cultures
  • B. Incubation of blood cultures at both 28°C and 35°C
  • C. Culture aspirates from bubos to MacConkey agar at room temperature
  • D. All of these options

Question 28

Question
SITUATION: Abdominal pain, fever, vomiting, and nausea prompted an elderly male to seek medical attention. A watery stool specimen producing no fecal leukocytes or erythrocytes was cultured and grew a predominance of gram-negative fermentative bacilli. The colonies were beta-hemolytic on blood agar and cream colored on MacConkey agar. The colonies were both oxidase and catalase positive. What is the most likely identification?
Answer
  • A. Aeromonas hydrophilia
  • B. Escherichia coli
  • C. Salmonella spp.
  • D. Shigella spp.

Question 29

Question
29. SITUATION: Several attendees of a medical conference in the Gulf coast area became ill after frequenting a seafood restaurant. A presumptive identification of Vibrio cholera was made after stool specimens from several subjects grew clear colonies on MacConkey agar and yellow colonies on TCBS agar. Which key tests would help eliminate Aeromonas and Plesiomonas spp.?
Answer
  • A. Mannitol fermentation, Na+ requirement
  • B. Oxidase, motility
  • C. Oxidase, nitrate
  • D. Hemolysis on blood agar, catalase

Question 30

Question
SITUATION: A group of elementary students became ill after eating undercooked ground beef prepared in the school cafeteria. The suspected pathogen, E. coli serotype 0157:H7, is usually recovered using which of the following media?
Answer
  • A. XLD agar
  • B. MacConkey agar
  • C. MacConkey agar with sorbitol
  • D. Hektoen agar

Question 31

Question
Biochemically, the Enterobacteriaceae are gram-negative rods that:
Answer
  • A. Ferment glucose, reduce nitrate to nitrite, and are oxidase negative
  • B. Ferment glucose, produce indophenol oxidase, and form gas
  • C. Ferment lactose and reduce nitrite to nitrogen gas
  • D. Ferment lactose and produce indophenol oxidase

Question 32

Question
The ortho-nitrophenyl-β-galactopyranoside (ONPG) test is most useful when differentiating:
Answer
  • A. Salmonella spp. from Pseudomonas spp.
  • B. Shigella spp. from some strains of Escherichia coli
  • C. Klebsiella spp. from Enterobacter spp.
  • D. Proteus vulgaris from Salmonella spp.

Question 33

Question
he Voges–Proskauer (VP) test detects which end product of glucose fermentation?
Answer
  • A. Acetoin
  • B. Nitrite
  • C. Acetic acid
  • D. Hydrogen sulfide

Question 34

Question
At which pH does the methyl red (MR) test become positive?
Answer
  • A. 7.0
  • B. 6.5
  • C. 6.0
  • D. 4.5

Question 35

Question
A positive Simmons citrate test is seen as a:
Answer
  • A. Blue color in the medium after 24 hours of incubation at 35°C
  • B. Red color in the medium after 18 hours of incubation at 35°C
  • C. Yellow color in the medium after 24 hours of incubation at 35°C
  • D. Green color in the medium after 18 hours of incubation at 35°C

Question 36

Question
In the test for urease production, ammonia reacts to form which product?
Answer
  • A. Ammonium citrate
  • B. Ammonium carbonate
  • C. Ammonium oxalate
  • D. Ammonium nitrate

Question 37

Question
Which of the following reagents is added to detect the production of indole?
Answer
  • A. p-Dimethylaminobenzaldehyde
  • B. Bromcresol purple
  • C. Methyl red
  • D. Cytochrome oxidase

Question 38

Question
Decarboxylation of the amino acids lysine, ornithine, and arginine results in the formation of:
Answer
  • A. Ammonia
  • B. Urea
  • C. CO2
  • D. Amines

Question 39

Question
Lysine iron agar (LIA) showing a purple slant and a blackened butt indicates:
Answer
  • A. E. coli
  • B. Citrobacter spp.
  • C. Salmonella spp.
  • D. Proteus spp.

Question 40

Question
Putrescine is an alkaline amine product of which bacterial enzyme?
Answer
  • A. Arginine decarboxylase
  • B. Phenylalanine deaminase
  • C. Ornithine decarboxylase
  • D. Lysine decarboxylase

Question 41

Question
Which genera are positive for phenylalanine deaminase?
Answer
  • A. Enterobacter, Escherichia, and Salmonella
  • B. Morganella, Providencia, and Proteus
  • C. Klebsiella and Enterobacter
  • D. Proteus, Escherichia, and Shigella

Question 42

Question
Kligler iron agar (KIA) differs from triple-sugar iron agar (TSI) in the:
Answer
  • A. Ratio of lactose to glucose
  • B. Ability to detect H2S production
  • C. Use of sucrose in the medium
  • D. Color reaction denoting production of acid

Question 43

Question
The malonate test is most useful in differentiating which members of the Enterobacteriaceae?
Answer
  • A. Shigella
  • B. Proteus
  • C. Salmonella subgroups 2, 3 (the former Arizona)
  • D. Serratia

Question 44

Question
Which genera of the Enterobacteriaceae are known to cause diarrhea and are considered enteric pathogens?
Answer
  • A. Enterobacter, Klebsiella, Providencia, and Proteus
  • B. Escherichia, Salmonella, Shigella, and Yersinia
  • C. Pseudomonas, Moraxella, Acinetobacter, and Aeromonas
  • D. Enterobacter, Citrobacter, and Morganella

Question 45

Question
An isolate of E. coli recovered from the stool of a patient with severe bloody diarrhea should be tested for which sugar before sending it to a reference laboratory for serotyping?
Answer
  • A. Sorbitol (fermentation)
  • B. Mannitol (oxidation)
  • C. Raffinose (fermentation)
  • D. Sucrose (fermentation)

Question 46

Question
Care must be taken when identifying biochemical isolates of Shigella because serological cross reactions occur with:
Answer
  • A. E. coli
  • B. Salmonella spp.
  • C. Pseudomonas spp.
  • D. Proteus spp.

Question 47

Question
Which species of Shigella is most commonly associated with diarrheal disease in the United States?
Answer
  • A. S. dysenteriae
  • B. S. flexneri
  • C. S. boydii
  • D. S. sonnei

Question 48

Question
Which of the following tests best differentiates Shigella species from E. coli?
Answer
  • A. Hydrogen sulfide, VP, citrate, and urease
  • B. Lactose, indole, ONPG, and motility
  • C. Hydrogen sulfide, MR, citrate, and urease
  • D. Gas, citrate, and VP

Question 49

Question
Which genera of Enterobacteriaceae are usually nonmotile at 36°C?
Answer
  • A. Shigella, Klebsiella, and Yersinia
  • B. Escherichia, Edwardsiella, and Enterobacter
  • C. Proteus, Providencia, and Salmonella
  • D. Serratia, Morganella, and Hafnia

Question 50

Question
Fever, abdominal cramping, watery stools, and fluid and electrolyte loss preceded by bloody stools 2–3 days before is characteristic of shigellosis but may also result from infection with:
Answer
  • A. Campylobacter spp.
  • B. Salmonella spp.
  • C. Proteus spp.
  • D. Yersinia spp.

Question 51

Question
Cold enrichment of feces (incubation at 4°C) in phosphate-buffered saline prior to subculture onto enteric media enhances the recovery of:
Answer
  • A. Enterotoxigenic E. coli
  • B. Salmonella paratyphi
  • C. Hafnia alvei
  • D. Y. enterocolitica

Question 52

Question
Which group of tests, along with colonial morphology on primary media, aids most in the rapid identification of the Enterobacteriaceae?
Answer
  • A. MR and VP, urease, and blood agar plate
  • B. Phenylalanine deaminase, urease, and CDC agar plate
  • C. Bacitracin, β-lactamase, and MacConkey agar plate
  • D. Indole, oxidase, MacConkey, and blood agar plates

Question 53

Question
A routine, complete stool culture procedure should include media for the isolation of E. coli O157:H7 as well as:
Answer
  • A. Salmonella, Shigella, Yersinia, Campylobacter, and Staphylococcus aureus
  • B. Vibrio cholerae, Brucella, and Yersinia spp.
  • C. S. aureus, group B streptococci, and group D streptococci
  • D. Clostridium difficile, Clostridium perfringens, and Yersinia spp.

Question 54

Question
Which group of tests best identifies the Morganella and Proteus genera?
Answer
  • A. Motility, urease, and phenylalanine deaminase
  • B. Malonate, glucose fermentation, and deoxyribonuclease (DNase)
  • C. Indole, oxidase, MR, and VP
  • D. Indole, citrate, and urease

Question 55

Question
Which group of tests best differentiates Enterobacter aerogenes from Edwardsiella tarda?
Answer
  • A. Motility, citrate, and urease
  • B. Hydrogen sulfide (H2S) production, sucrose fermentation, indole, and VP
  • C. Lysine decarboxylase, urease, and arginine dihydrolase
  • D. Motility, H2S production, and DNase

Question 56

Question
Enterobacter sakazakii can best be differentiated from Enterobacter cloacae by which of the following characteristics?
Answer
  • A. Yellow pigmentation and negative sorbitol fermentation
  • B. Pink pigmentation and positive arginine dihydrolase
  • C. Yellow pigmentation and positive urease
  • D. H2S production on TSI

Question 57

Question
Members of the genus Cedecea are best differentiated from Serratia spp. by which test result?
Answer
  • A. Positive motility
  • B. Positive urease
  • C. Positive phenylalanine deaminase
  • D. Negative DNase

Question 58

Question
Which of the following organisms is often confused with the Salmonella species biochemically and on plated media?
Answer
  • A. E. coli
  • B. Citrobacter freundii
  • C. Enterobacter cloacae
  • D. Shigella dysenteriae

Question 59

Question
A gram-negative rod is recovered from a catheterized urine sample from a nursing home patient. The lactose-negative isolate tested positive for indole, urease, ornithine decarboxylase, and phenylalanine deaminase and negative for H2S. The most probable identification is:
Answer
  • A. Edwardsiella spp.
  • B. Morganella spp.
  • C. Ewingella spp.
  • D. Shigella spp.

Question 60

Question
Which single test best separates Klebsiella oxytoca from K. pneumoniae?
Answer
  • A. Urease
  • B. Sucrose
  • C. Citrate
  • D. Indole

Question 61

Question
Which of the following organisms, found in normal fecal flora, may be mistaken biochemically for the genus Yersinia?
Answer
  • A. Klebsiella spp.
  • B. Proteus spp.
  • C. E. coli
  • D. Enterobacter spp.

Question 62

Question
Why might it be necessary for both pink (lactose-positive) and colorless (lactose-negative) colonies from an initial stool culture on MacConkey agar to be subcultured and tested further for possible pathogens?
Answer
  • A. Most Shigella strains are lactose positive
  • B. Most Salmonella strains are maltose negative
  • C. Most Proteus spp. are lactose negative
  • D. Pathogenic E. coli can be lactose positive or lactose negative

Question 63

Question
Which agar that is used for routine stool cultures is the medium of choice for the isolation of Yersinia strains from stool specimens?
Answer
  • A. Salmonella–Shigella agar
  • B. Hektoen enteric agar
  • C. MacConkey agar
  • D. CNA agar

Question 64

Question
Which organism is sometimes mistaken for Salmonella and will agglutinate in Salmonella polyvalent antiserum?
Answer
  • A. C. freundii strains
  • B. Proteus mirabilis strains
  • C. S. sonnei strains
  • D. E. coli

Question 65

Question
A bloody stool cultured from a 26-year-old woman after 3 days of severe diarrhea showed the following results at 48 hours after being plated on the following media: MacConkey agar: little normal flora with many non–lactose-fermenting colonies Hektoen enteric agar: many blue-green colonies Campylobacter blood agar and C. difficile agar: no growth Clear colonies (from MacConkey agar) tested negative for oxidase, indole, urease, motility, and H2S The most likely identification is:
Answer
  • A. Shigella spp.
  • B. Salmonella spp.
  • C. Proteus spp.
  • D. E. coli

Question 66

Question
Which of the following organisms are generally positive for β-galactosidase?
Answer
  • A. Salmonella spp.
  • B. Shigella spp.
  • C. Proteus spp.
  • D. E. coli

Question 67

Question
In the Kauffmann–White schema, the combined antigens used for serological identification of the Salmonella spp. are:
Answer
  • A. O antigens
  • B. H antigens
  • C. Vi and H antigens
  • D. O, Vi, and H antigens

Question 68

Question
The drugs of choice for treatment of infections with Enterobacteriaceae are:
Answer
  • A. Aminoglycosides, trimethoprim–sulfamethoxazole, third-generation cephalosporins
  • B. Ampicillin and nalidixic acid
  • C. Streptomycin and isoniazid
  • D. Chloramphenicol, ampicillin, and colistin

Question 69

Question
The Shiga-like toxin (verotoxin) is produced mainly by which Enterobacteriaceae?
Answer
  • A. Klebsiella pneumoniae
  • B. E. coli
  • C. Salmonella typhimurium
  • D. Enterobacter cloacae

Question 70

Question
Infections caused by Yersinia pestis are rare in the United States. Those cases that do occur are most frequently located in which region?
Answer
  • A. New Mexico, Arizona, and California
  • B. Alaska, Oregon, and Utah
  • C. North and South Carolina and Virginia
  • D. Ohio, Michigan, and Indiana

Question 71

Question
A leg culture from a nursing home patient grew gram-negative rods on MacConkey agar as pink to dark pink oxidase-negative colonies. Given the following results, which is the most likely organism? TSI = A/A Indole = Neg MR = Neg VP = + Citrate = + H2S = Neg Urease = + Motility = Neg Antibiotic susceptibility: resistant to carbenicillin and ampicillin
Answer
  • A. Serratia marcescens
  • B. Proteus vulgaris
  • C. Enterobacter cloacae
  • D. Klebsiella pneumoniae

Question 72

Question
Four blood cultures were taken over a 24-hour period from a 20-year-old woman with severe diarrhea. The cultures grew motile (room temperature), gram-negative rods. A urine specimen obtained by catheterization also showed gram-negative rods, 100,000 col/mL. Given the following results, which is the most likely organism? TSI = A/A gas Indole = + VP = Neg MR = + H2S = Neg Citrate = Neg Urease = Neg Lysine decarboxylase = + Phenylalanine deaminase = Neg
Answer
  • A. Proteus vulgaris
  • B. Salmonella typhi
  • C. Yersinia enterocolitica
  • D. E. coli

Question 73

Question
A stool culture from a 30-year-old man suffering from bloody mucoid diarrhea gave the following results on differential enteric media: MacConkey agar = clear colonies XLD agar = clear colonies Hektoen agar = green colonies Salmonella–Shigella agar = small, clear colonies Which tests are most appropriate for identification of this enteric pathogen?
Answer
  • A. TSI, motility, indole, urease, Shigella typing with polyvalent sera
  • B. TSI, motility, indole, lysine, Salmonella typing with polyvalent sera
  • C. TSI, indole, MR, VP, citrate
  • D. TSI, indole, MR, and urease

Question 74

Question
A leg-wound culture from a hospitalized 70-year-old diabetic man grew motile, lactose-negative colonies on MacConkey agar. Given the following biochemical reactions at 24 hours, what is the most probable organism? H2S (TSI) = Neg Indole = Neg MR = Neg VP = + DNase = + Citrate = + Urease = Neg Phenylalanine deaminase = Neg Ornithine and lysine decarboxylase = + Arginine decarboxylase = Neg Gelatin hydrolysis = +
Answer
  • A. Proteus vulgaris
  • B. Serratia marcescens
  • C. Proteus mirabilis
  • D. Enterobacter cloacae

Question 75

Question
Three blood cultures taken from a 30-year-old cancer patient receiving chemotherapy and admitted with a urinary tract infection grew lactose-negative, motile, gram-negative rods prior to antibiotic therapy. Given the following biochemical reactions, which is the most likely organism? H2S (TSI) = + Indole = + MR = + VP = Neg Citrate = Neg Urease = + DNase = + Phenylalanine deaminase = + Gelatin hydrolysis = + Ornithine decarboxylase = Neg
Answer
  • A. Proteus vulgaris
  • B. Proteus mirabilis
  • C. Serratia marcescens
  • D. Klebsiella pneumoniae

Question 76

Question
Three consecutive stool cultures from a 25-year-old male patient produced scant normal fecal flora on MacConkey and Hektoen agars. However, colonies on CIN agar (cefsulodin–irgasan–novobiocin) displayed “bulls-eye” colonies after 48 hours incubation. The patient had been suffering from enterocolitis with fever, diarrhea, and abdominal pain for 2 days. What is the most likely identification of this gram-negative rod?
Answer
  • A. E. coli
  • B. Proteus mirabilis
  • C. Yersinia enterocolitica
  • D. Klebsiella pneumoniae

Question 77

Question
A 6-year-old female patient was admitted to the hospital following 2 days of severe diarrhea. Cultures from three consecutive stool samples contained blood and mucus. Patient history revealed a hamburger lunch at a fast-food restaurant 3 days earlier. Which pathogen is most likely responsible for the following results? Growth on: XLD agar = yellow colonies HE agar = yellow colonies Mac agar = light pink and dark pink colonies Mac with sorbitol agar – few dark pink and many colorless colonies
Answer
  • A. Salmonella spp.
  • B. Shigella spp.
  • C. E. coli O157:H7
  • D. Yersinia enterocolitica

Question 78

Question
A leg culture from a nursing home patient grew gram-negative rods on MacConkey agar as pink to dark pink oxidase-negative colonies. Given the following results, which is the most likely organism? TSI = A/A Indole = Neg MR = Neg VP = + Citrate = + H2S = Neg Urease = + Motility = Neg Antibiotic susceptibility: resistant to carbenicillin and ampicillin
Answer
  • A. Serratia marcescens
  • B. Proteus vulgaris
  • C. Enterobacter cloacae
  • D. Klebsiella pneumoniae

Question 79

Question
Four blood cultures were taken over a 24-hour period from a 20-year-old woman with severe diarrhea. The cultures grew motile (room temperature), gram-negative rods. A urine specimen obtained by catheterization also showed gram-negative rods, 100,000 col/mL. Given the following results, which is the most likely organism? TSI = A/A gas Indole = + VP = Neg MR = + H2S = Neg Citrate = Neg Urease = Neg Lysine decarboxylase = + Phenylalanine deaminase = Neg
Answer
  • A. Proteus vulgaris
  • B. Salmonella typhi
  • C. Yersinia enterocolitica
  • D. E. coli

Question 80

Question
A stool culture from a 30-year-old man suffering from bloody mucoid diarrhea gave the following results on differential enteric media: MacConkey agar = clear colonies; XLD agar = clear colonies; Hektoen agar = green colonies; Salmonella–Shigella agar = small, clear colonies Which tests are most appropriate for identification of this enteric pathogen?
Answer
  • A. TSI, motility, indole, urease, Shigella typing with polyvalent sera
  • B. TSI, motility, indole, lysine, Salmonella typing with polyvalent sera
  • C. TSI, indole, MR, VP, citrate
  • D. TSI, indole, MR, and urease

Question 81

Question
A leg-wound culture from a hospitalized 70-year-old diabetic man grew motile, lactose-negative colonies on MacConkey agar. Given the following biochemical reactions at 24 hours, what is the most probable organism? H2S (TSI) = Neg Indole = Neg MR = Neg VP = + DNase = + Citrate = + Urease = Neg Phenylalanine deaminase = Neg Ornithine and lysine decarboxylase = + Arginine decarboxylase = Neg Gelatin hydrolysis = +
Answer
  • A. Proteus vulgaris
  • B. Serratia marcescens
  • C. Proteus mirabilis
  • D. Enterobacter cloacae
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