Gentics -RS Q's

If a person develops a familial cancer, it is likely that they were born with the gentotype....

What is a Roundup Ready transgenic organism?

An individual has a mutation in the proto-oncogene Ras. What type of mutation has likely occured? A. Gain of Function B. Loss of Function C.Recessive D. Dominant

Due to this mutation, what is the abnormal function of the mutant Ras? The Ras is [blank_start]continously on[blank_end] (Bound of [blank_start]GTP[blank_end]) because it lots its [blank_start]GDP[blank_end] site (which brings it back down to an [blank_start]inactive state[blank_end]).

List one of the difference between familial breast cancer and sporadic breast cancer: Familial breast cancer accounts for [blank_start]10%[blank_end] of the cancer developed and sporadic cancer accounts for [blank_start]80%[blank_end] of cancer developed.

Can you manufacture human protein using bacteria? Why or why not? You [blank_start]can[blank_end] with [blank_start]recombinant[blank_end] plasmids, but you need [blank_start]bacteria[blank_end] that has [blank_start]post-translational modifications[blank_end] like in Eukaryotes.

Gene cloning happens in this order: Purify DNA of interest for ligation Cut vector and ligate in gene Transform recombinant plasmid into competent bacteria Select for transformants

What is PCR used for?

Name the three steps of PCR and briefly describe their functions: [blank_start]Denaturation[blank_end]- Break [blank_start]double stranded[blank_end] DNA bonds with heat into [blank_start]single stranded[blank_end] DNA. Add [blank_start]primers[blank_end] [blank_start]Annealing[blank_end]- Seal [blank_start]primers[blank_end] to specific sequence of DNA [blank_start]Extension[blank_end]- Have the [blank_start]Single stranded[blank_end] DNA go through [blank_start]elongation[blank_end] to create a [blank_start]double stranded[blank_end] DNA piece.

There is no issue with PCR. The starting piece of DNA sequence that is selected to be multiplied will always be perfect and therefore, make perfect copies.

What is sgRNA?

What is sgRNA used for in CRISPR-Cas 9?

What is the result of the following conjugations? F+ x F- = [blank_start]F+[blank_end] + [blank_start]F+[blank_end] Hfr x F- = [blank_start]Hfr[blank_end] + [blank_start]F- recombinant[blank_end]

The transfer of naked DNA to a recipient bacterial cell is called

Naked DNA can be taken up by any nearby bacteria, but requires cell to cell contact between the donor and the recipient.

DNA can also be transferred by conjugation, which requires a bacteriophage.

Approximately 80% of caner cases arise from germ-line mutation

Lederberg and Tatum's work with the autotrophic bacteria demonstrated transduction.

R plasmids are a type of F plasmid that can confer antibiotic resistance.

Leukemias and lymphnodes are examples of solid tumors

Two disease-free parents have a child with Cystic Fibrosis. What is the probability that their next child will be a carrier?

If a genes has 4 alleles in a population, how many different genotypes and phenotypes are possible? Show your work!

Where are the genes for sex-limited traits located?

In incomplete dominance, a likely phenotypic ratio resulting when a heterozygous is selfed in a monohybrid cross would be 3:1.

X-chromosome inactivation is important in explaining the unexpected phenotype of females who are homozygous for X-linked genes

In an X-linked recessive disease, more males are affected than females.

Which type of disease is most commonly embryonic lethal? Choose all that apply

If the GC content of a double stranded DNA molecule is 60%: What is the molar precentage of purines?

If the GC content of a double stranded DNA molecule is 60%: If this were a single stranded DNA molecule, what would the molar precentage of T be?

If a DNA molecule is 150 bp long, what is the approximate length in Angstroms or nm. Choose all that apply

Origins of replication are likely found in GC rich regions.

In eukaryotes, circular chromosomes have many origins of replication.