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Clinlab 4 quiz

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Clinlab 4 quiz
Zachariah Atteberry
Quiz by Zachariah Atteberry, updated more than 1 year ago
Zachariah Atteberry
Created by Zachariah Atteberry over 8 years ago
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Resource summary

Question 1

Question
Which is true of the Columbia colistin-nalidixic acid agar?
Answer
  • Selective for gram - only
  • Selective for gram + only
  • Uses crystal violet light to inhibit gram + and is selective for gram -
  • Good for gram + and gram - only
  • Works against fungi and gram - bacteria
  • Uses crystal violet light to inhibit gram - and use selective for gram +

Question 2

Question
Which is true of the MacConkey II agar?
Answer
  • Selective for gram + only
  • Uses crystal violet light to inhibit gram + and is selective for gram -
  • Good for gram + and gram - only
  • Works against fungi and gram - bacteria
  • Uses crystal violet light to inhibit gram - and use selective for gram +
  • Selective for gram - only

Question 3

Question
In regards to the MAC agar, does it differentiate?
Answer
  • Differentiates between lactose and non lactose fermenters, turns colonies purple
  • Differentiates between lactose and non lactose fermenters, turns colonies salmon
  • Differentiates between lactose and non lactose fermenters, turns colonies blue/purple
  • No it does not

Question 4

Question
Which plate inhibits the swarming of Proteus spp?
Answer
  • MacConkey 2 agar
  • Columbia colistin-nalidixic acid
  • Thioglycollate broth
  • Mycosel agar
  • Chocolate agar
  • Mueller-hinton agar

Question 5

Question
Phenylethyl alcohol is...
Answer
  • Selective for gram -
  • Selective for gram +
  • Detects both gram + and gram - in addition to fungi
  • Selective for gram - and fungi
  • Selective for gram - and uses crystal violet to inhibit gram +

Question 6

Question
Phenylethyl alcohol is differential
Answer
  • True
  • False

Question 7

Question
Which agar is the only agar approved for antibotic sensitivity testing?
Answer
  • MH
  • MAC
  • Levine
  • Hektoen enteric agar
  • Salmonella-shigella agar
  • MacConkey 2 agar

Question 8

Question
In regards to Mueller Hinton agars... which is true?
Answer
  • Can be enriched with blood for more fastidious organisms
  • Only gram -
  • only gram +
  • General use and is enriched with blood for more fastidious organisms
  • Only gram + and and is enriched with blood for more fastidious organisms
  • General use and is enriched with blood for more non- fastidious organisms
  • Only gram + and and is enriched with blood for more non-fastidious organisms

Question 9

Question
Which will produce a black pigment in hydrogen sulfide producing colonies?
Answer
  • SS
  • MAC
  • MH
  • Hektoen enteric agar

Question 10

Question
In regards to the SS plate, what does it do?
Answer
  • Selects for pathogenic enteric gram -
  • Selects for pathogenic enteric gram +
  • Selects for pathogenic enteric gram + and gram -
  • Only selects for gram - and gram +
  • Selects for gram + and dermatophytes

Question 11

Question
Salmonella-Shigella agar differentiates on the basis of lactose fermentation
Answer
  • True
  • False

Question 12

Question
Hektoen enteric agars... are?
Answer
  • Selective for gram - and differential/turns salmon if lactose fermenter
  • Selective for gram + and differential/turns salmon if lactose fermenter
  • Gram + and gram - and differential/turns black/blue if lactose fermenter
  • Selective for gram + and turns blue/black if lactose fermenter
  • Neither selective or differential

Question 13

Question
Levine agar plates...
Answer
  • Selective for gram - and is differential for lactose fermenters...turns blue/black
  • Selective for gram + and is differential for lactose fermenters...turns black
  • Selective for gram + and is differential for lactose fermenters...turns blue
  • Selective for gram - and is differential for lactose fermenters...turns salmon

Question 14

Question
Which does not have the option of 5% sheep blood being added or already has 5% sheep blood?
Answer
  • BAP
  • MAC
  • CNA
  • Trypticase soy agar
  • Columbia-colistin-nalidixic acid
  • TSA

Question 15

Question
Alpha plate
Answer
  • Partial hemolysis - green slime around colony
  • Complete hemolysis - clear zone around colony
  • Double (beta inside, alpha around it)
  • No hemolysis or change

Question 16

Question
Gamma plate
Answer
  • No hemolysis or change
  • Double (beta inside, alpha around it)
  • Complete hemolysis - clear zone around colony
  • Partial hemolysis - green slime around colony

Question 17

Question
Beta plate
Answer
  • Partial hemolysis - green slime around colony
  • Complete hemolysis - clear zone around colony
  • Double (beta inside, alpha around it)
  • No hemolysis or change

Question 18

Question
Delta plate
Answer
  • No hemolysis or change
  • Double (beta inside, alpha around it)
  • Complete hemolysis - clear zone around colony
  • Partial hemolysis - green slime around colony

Question 19

Question
Which is used to ID brucella?
Answer
  • Chocolate agar
  • MAC
  • BAP
  • TSA
  • MH
  • Mueller-hinton agar

Question 20

Question
Which has lysed blood cells?
Answer
  • CA
  • OF
  • NA
  • TSA
  • BAP

Question 21

Question
Which is used to ID bacteria like enterococci?
Answer
  • BE
  • BHI
  • NA
  • OF
  • TSA
  • BAP

Question 22

Question
A positive reaction with the bile esculin agar is?
Answer
  • Dark brown color
  • Blue/black
  • yellow/green
  • Green
  • Black
  • Salmonella

Question 23

Question
Brain heart infusion is a?
Answer
  • Enriched broth used for antibiotic sensitivity tests
  • Determines dextrose in gram - bacteria
  • Sterile solution for diluting gram - bacteria for API testing
  • Determines urease production of bacteria
  • ID bacteria like enterococci

Question 24

Question
Nutrient agar slant is used for?
Answer
  • Fastidous organisms
  • Non-fastidous organisms
  • Enterococci
  • Sterile solution for diluting gram - bacteria for API testing
  • Determines urease production of bacteria
  • Enriched broth used for antibiotic sensitivity tests

Question 25

Question
Oxidation fermentation medium with dextrose is used for?
Answer
  • Determines urease production of bacteria
  • Sterile solution for diluting gram + bacteria for API testing
  • Sterile solution for diluting gram - bacteria for API testing
  • Enriched broth used for antibiotic sensitivity tests
  • Determines dextrose in gram - bacteria
  • Determines dextrose in gram + bacteria

Question 26

Question
Sodium chloride 0.85% is used for?
Answer
  • Determines dextrose in gram + bacteria
  • Determines dextrose in gram - bacteria
  • Sterile solution for diluting gram + bacteria for API testing
  • Sterile solution for diluting gram - bacteria for API testing
  • Determines urease production of gram - bacteria
  • Determines urease production of gram + bacteria

Question 27

Question
What can grow most anything as a general use broth?
Answer
  • THIO w/o indicator 135c
  • TSB
  • UREA
  • GM
  • Mycosel agar with 135C
  • THIO w/ indicator 135c

Question 28

Question
What is used for blood cultures and sterility testing?
Answer
  • Trypticase soy broth
  • TSB
  • THIO
  • Thioglycollate broth
  • Both TSB and Trypticase soy broth
  • Both TSA and Trypticase soy agar
  • Both TSA and Trypticase soy broth

Question 29

Question
What color does urea agar slant (UREA) turn and for what reason?
Answer
  • Determines urease production of bacteria... turns red
  • Determines urease production of bacteria... turns blue
  • Determines urease production of bacteria... turns yellow
  • Determines urease production of bacteria... turns green
  • Lactose fermenter... turns salmon
  • Lactose fermenter.... turns blue/black

Question 30

Question
Which is known for selective enrichment medium for Salmonella and Shigella spp.
Answer
  • Gram negative broth
  • Bile esculin agar
  • Brain-heart infusion
  • Nutrient agar slant
  • Oxidation fermentation medium with dextrose
  • Salmonella-Shigella Agar
  • SS

Question 31

Question
Which is used mostly for fecal culturing?
Answer
  • GN
  • TSB
  • UREA
  • THO
  • Mycosel agar

Question 32

Question
The motility test is used to?
Answer
  • Blood cultures and sterility testing along with test bacteria motility
  • fecal testing and demonstrate motility of bacteria
  • fungal culture and demonstrate motility of bacteria
  • Demonstrate motility of bacteria
  • Sterile solution for diluting gram - bacteria for API testing
  • Sterile solution for diluting gram + bacteria for API testing

Question 33

Question
Which agar is used as a selective fungal medium?
Answer
  • GN
  • Gram negative broth
  • Thioglycollate broth
  • Trypticase soy broth
  • BE
  • BHI
  • Mycosel agar

Question 34

Question
Which contains cycloheximide and chloramphenicol to inhibit bacterial growth?
Answer
  • Mycosel agar
  • Thioglycollate broth
  • Trypticase soy broth
  • Urea agar slant
  • TSB
  • CA
  • BE
  • BHI

Question 35

Question
What contains gentamicin and chlortetracycline to isolate pathogenic fungi?
Answer
  • DTM
  • DTS
  • GN
  • BE
  • BHI
  • OF
  • NA

Question 36

Question
What causes color change in the presence of acid producing, rapidly growing fungi... and what color does it change?
Answer
  • DTM....phenol red
  • DTM... blue
  • DTM....green/blue
  • Dermatophyte test medium.... green/yellow
  • THIO w/indicator 135 c
  • GN
  • MME

Question 37

Question
Which is not a precaution to follow for DTM?
Answer
  • Dark area
  • Room temp
  • Keep the cap loose
  • Keep up to 60 days

Question 38

Question
What is 1 side DTM and the other side RSM?
Answer
  • Derm Duet
  • Derm-Duo
  • DDTM
  • Mycosel agar
  • GM

Question 39

Question
What turns blue/green with quick growing fungus?
Answer
  • Derm-Duet
  • DTM
  • Dermatophyte grow medium
  • GM
  • Thioglycollate broth
  • trypticase soy broth

Question 40

Question
[blank_start]Agar[blank_end] is semisolid media [blank_start]Broth[blank_end] is liquid media [blank_start]Plate[blank_end] is a flat, round container of agar [blank_start]Tube[blank_end] is screwtop container which can be broth or agar [blank_start]Slant[blank_end] is tube of agar that has gel at an angle
Answer
  • Agar
  • Broth
  • Plate
  • Tube
  • Slant

Question 41

Question
Which is not one of the three things you should do with all media in a clinic?
Answer
  • Prevent condensation but incubating upside down
  • Check all media for accidental contamination
  • Check all expiration dates
  • Always keep the lid screwed on tight for all media

Question 42

Question
[blank_start]Nutritive[blank_end] will grow all types of bacteria and some fungi [blank_start]Differential[blank_end] is how you differentiate between certain bacteria types [blank_start]Selective[blank_end] will only grow certain bacteria types
Answer
  • Nutritive
  • Differential
  • Selective

Question 43

Question
Which are selective for gram +?
Answer
  • Columbia colistin nalidixic acid
  • Phenylethyl alcohol
  • MAC
  • Mueller-Hinton agar
  • Hektoen enteric agar
  • Levine
  • Salmonella-Shigella agar (SS)
  • NA

Question 44

Question
Which are selective for gram -?
Answer
  • CNA
  • MAC
  • PEA
  • SS
  • MH
  • Levine
  • Hektoen enteric agar

Question 45

Question
Please identify the abbreviations for the following plates. The columbia colistin nalidixic acid which is selective for gram + bacteria is also known as [blank_start]CNA[blank_end] The MacConkey II agar plate is also know as [blank_start]MAC[blank_end] Mueller-Hinton agar is also known as [blank_start]MH[blank_end] Salmonella-Shigella agar is also known as [blank_start]SS[blank_end] Trypticase soy agar known as TSA without blood is known as [blank_start]BAP[blank_end] with 5% blood Chocolate agar is known as [blank_start]CA[blank_end]
Answer
  • CNA
  • MAC
  • MH
  • SS
  • BAP
  • CA

Question 46

Question
Identify the abbreviations. Bile esculin agar also known as [blank_start]BE[blank_end] Brain heart infusion as known as [blank_start]BHI[blank_end] Nutrient agar slant also known as [blank_start]NA[blank_end] Oxidation fermentation medium with dextrose also known as [blank_start]OF[blank_end] Thioglycollate broth also known as [blank_start]Thio[blank_end] Gram negative broth also known as [blank_start]GN[blank_end] Dermatophyte test medium also known as [blank_start]DTM[blank_end]
Answer
  • BE
  • BHI
  • NA
  • OF
  • Thio
  • GN
  • DTM
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