Reprogramming

Taken from Ralston, A. and J. Rossant (2010). "The genetics of induced pluripotency." Reproduction 139(1): 35-44.

Plate cells at density of 500,000 onto 10cm dishes - 6 well plate = 9.61cm2 

Day 1

Days 2-4 

Day 5

Day 6

Day 7-11

Day 12-16

Day 17

Days 18+

Scaling up of selected clones

Pick clones/colonies

Grow on FIAU

Allow for turn-over of vector

Electroporation

New Page

Piggy Bac excision