1956792
Pregunta 1
Pregunta
Pasteur is attributed with which one of the following discoveries?
Respuesta
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Was the first to see microorganisms under a microscope
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First to discover and use agar
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That there could be life without air
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The first chemical antibiotics
Pregunta 2
Pregunta
The length of a typical E. coli cell is approximately?
Respuesta
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20 μm
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2 μm
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200 nm
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20 nm
Pregunta 3
Pregunta
With respect to differences between members of the archaeal and bacterial
domains/kingdoms, which is the CORRECT statement?
Respuesta
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Both have peptidoglycan in their cell walls
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Only some Archaea are capable of methanogenesis
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Archaea are sensitive to β-lactam antibiotics
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Bacteria have similar RNA polymerase to that of Archaea
Pregunta 4
Pregunta
Which statement is CORRECT with respect to the E. coli chromosome?
Respuesta
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It is negatively supercoiled
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It is positively supercoiled
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There are typically several origins of replication
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It consists of over 50 million base pairs
Pregunta 5
Pregunta
A culture of E. coli is diluted by a factor of x 10-5 and, after plating out 100 µl on an agar plate, 78 colonies grew after incubation at 37oC overnight. The original culture
therefore contained how many viable E. coli cells per ml?
Respuesta
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7.8 x 10(7)
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7.8 x 10(8)
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7.8 x 10(6)
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7.8 x 10(5)
Pregunta 6
Pregunta
Bacterial flagella bring about cell motion by which mechanism?
Respuesta
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Wave-like movement of the flagellum
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Rotation of the cell
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Rotation of the flagellum in one direction only
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Rotation of the flagellum that is reversible
Pregunta 7
Pregunta
The Bacille-Calmette-Guérin (BCG) vaccine is:
Respuesta
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Heat-killed Mycobacterium tuberculosis
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Attenuated live Mycobacterium tuberculosis
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Mycobacterium bovis
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Attenuated live Mycobacterium bovis
Pregunta 8
Pregunta
Which of the following is only transmitted by direct person-to-person contact?
Respuesta
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Malaria
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Plague
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Gonorrhoea
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. Tuberculosis
Pregunta 9
Pregunta
Photoautotophs use:
Respuesta
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Light as an energy source and CO2 as a carbon source
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Light as an energy and glucose as a carbon source
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CO2 as both an energy source and a carbon source
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All of the above.
Pregunta 10
Pregunta
Biochemical synthesis of new cell material is called:
Respuesta
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Metabolism.
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Anabolism.
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Catabolism.
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Synthatabolism.
Pregunta 11
Pregunta
Carl Woese of the University of Illinois and his collaborators identified two major branches of prokaryotic evolution. What was the basis for dividing prokaryotes into two domains?
Respuesta
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Microscopic examination of the staining characteristics of the cell wall.
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. Metabolic characteristics such as the production of methane gas
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Ecological characteristics such as the ability to survive extreme temperatures
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Molecular characteristics such as ribosomal RNA sequences
Pregunta 12
Pregunta
Incorporation of atmospheric N2 to NH4+ occurs via the process of:
Respuesta
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Nitrogen fixation.
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Nitrification.
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Denitrification.
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None of the above, atmospheric N2 cannot be incorporated into NH4+
Pregunta 13
Pregunta
Which of these environmental factors influence microbial growth?
Respuesta
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pH
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Water potential
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Oxygen
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All of the above
Pregunta 14
Pregunta
The cardinal temperatures are the:
Respuesta
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Minimum temperatures
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Optimum temperatures
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Maximum temperatures
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Minimum, optimum, and maximum temperatures taken together.
Pregunta 15
Pregunta
‘Necrosis’ is a symptom of infection of a plant with a pathogenic organism. Necrosis is:
Respuesta
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Yellowing of infected or surrounding tissue
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Uncontrolled cell division resulting in the formation of a gall
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Death of infected or surrounding cells
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Premature leaf fall
Pregunta 16
Pregunta
Phytophthora infestans is a fungus-like organism which:
Respuesta
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Causes disease of a wide range of woody hosts
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Causes potato blight
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Causes ringworm
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Causes black sigatoka disease of banana
Pregunta 17
Pregunta
Bacterial genes:
Respuesta
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often contain introns
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often organised in operons
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always encode proteins
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always shorter than 1,000 base pairs
Pregunta 18
Pregunta
The Bacterial chromosome is:
Respuesta
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Circular DNA
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Linear DNA
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Either circular or linear DNA
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Double stranded RNA
Pregunta 19
Pregunta
The genome of measles virus is composed of :
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Double stranded DNA
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Single stranded DNA
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Single stranded RNA
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DNA and RNA
Pregunta 20
Pregunta
The poliovirus mature virus particle:
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Contains 240 copies of a single protein
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Contains 60 copies of four proteins
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Has a dsDNA genome
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Has a single stranded negative–sense genome
Pregunta 21
Pregunta
With regard to the differences between bacterial and eukaryotic cells which statement is the CORRECT one?
Respuesta
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Bacterial cells contain mitochondria
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Bacterial cells are usually larger than eukaryote cells
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Bacteria do not contain a chromosome
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Only bacterial cells are sensitive to β-lactam antibiotics
Pregunta 22
Pregunta
Which statement is CORRECT with respect to the E.coli chromosome?
Respuesta
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It is negatively supercoiled
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It is positively supercoiled
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There are typically several origins of replication
-
It consists of over 50 million base pairs
Pregunta 23
Pregunta
Which statement is CORRECT with respect to Lipopolysaccharide (LPS)?
Respuesta
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LPS is found in Gram positive cells
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LPS is found in Gram negative cells in the cytoplasmic membrane
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LPS is found in the outer-membrane of Gram negative cells.
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LPS is found inside all bacterial cells
Pregunta 24
Pregunta
A culture of E. coli is diluted by a factor of 10(-5) and, after plating out 100 µl on an
agar plate, 142 colonies grew after incubation at 37oC overnight. The original
culture therefore contained how many viable E. coli cells per ml?
Respuesta
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1.42 x 10(7)
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1.42 x 10(8)
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1.42 x 10(6)
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1.42 x 10(5)
Pregunta 25
Pregunta
With respect to differences between members of the archaeal and bacterial
domains/kingdoms, which is the CORRECT statement?
Respuesta
-
Both have peptidoglycan in their cell walls
-
Only some Archaea are capable of methanogenesis
-
Archaea are sensitive to β-lactam antibiotics
-
Bacteria have similar RNA polymerase to that of Archaea
Pregunta 26
Pregunta
Which statement is CORRECT with respect to peptidoglycan?
Respuesta
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There are multiple molecules of peptidoglycan in bacterial cell walls
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Only Gram positive cells possess peptidoglycan
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There is only a single molecule of peptidoglycan in a bacterial cell wall
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Peptidoglycan is only found in the Gram negative cell wall
Pregunta 27
Pregunta
Which of the following is transmitted to humans by arthropods?
Respuesta
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Tuberculosis
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Malaria
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Cholera
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Typhoid
Pregunta 28
Pregunta
The antibiotic rifampicin interferes with which of the following?
Respuesta
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Peptidoglycan synthesis
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DNA gyrase
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Lipid synthesis
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RNA polymerase
Pregunta 29
Pregunta
Catalase is indispensable to
Respuesta
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Obligate anaerobes
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Aerotolerant anaerobes
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Aerobes
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All of the above
Pregunta 30
Pregunta
The CORRECT way to write this microbe’s name is
Respuesta
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bacillus subtilis (italics)
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Bacillus subtilis
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Bacillus Subtilis (italics)
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Bacillus subtilis (italics)
Pregunta 31
Pregunta
Chemical energy is released when organic or inorganic compounds are:
Respuesta
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Reduced
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Incorporated into larger molecules
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Oxidized
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Synthesized
Pregunta 32
Pregunta
A microbe isolated from deep in the ocean could best be characterized as a:
Respuesta
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Psychrophile.
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Mesophile.
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Barophile and psychrophile
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Barophile.
Pregunta 33
Pregunta
Which of the following is both a selective and a differential medium?
Respuesta
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Nutrient broth.
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MacConkey agar.
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Blood agar.
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Tryptic soya broth.
Pregunta 34
Pregunta
The signature sequence is used extensively in:
Respuesta
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Developing phylogenetic probes for fluorescent in situ hybridisation.
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Analyzing the G+C content of DNA.
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Isolating microorganisms from the environment.
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All of the above
Pregunta 35
Pregunta
Mature virus particles (Virions) ALWAYS contain:
Respuesta
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non-structural proteins
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a protease enzyme
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a DNA or RNA genome
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a reverse transcriptase
Pregunta 36
Pregunta
Viruses may encode their genomes as
Respuesta
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DNA
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RNA
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negative strand RNA
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All of the above
Pregunta 37
Pregunta
Organisms belonging to the genus Phytophthora are:
Respuesta
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True fungi
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Fungus-like organisms
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Slime moulds
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Rusts
Pregunta 38
Pregunta
Which of the following is NOT a correct statement about true fungi
Respuesta
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True fungi are eukaryotic
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All true fungi are multi-cellular
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True fungi are heterotrophic
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Most true fungi have chitin cell walls
Pregunta 39
Pregunta
With regards to bacterial plasmids which statement is the CORRECT one?
Respuesta
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A plasmid is a regulatory gene
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A plasmid is a short RNA molecule
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A plasmid is an extra chromosomal DNA molecule
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A plasmid is a non coding region found in some bacterial genomes
Pregunta 40
Pregunta
A Wild-type bacterial strain is:
Respuesta
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A strain with a limited number of mutations
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An arbitrarily chosen strain
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A strain without plasmids
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A strain which is sensitive to antibiotics
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