MCB Midterm Summary

A/an _____________ vector quantifies the expression of a gene to measure the activity of regulatory elements.

An expression vector is used to _________.

Which of the following is NOT an essential component of a plasmid?

What would not be found in a eukaryotic expression vector?

The expression of the _______ gene is quantified to study the activity of a regulatory sequence. In which type of vector would you find this gene?

Which is NOT involved in the construction of cDNA libraries?

What is the role of ribonuclease H in the synthesis of cDNA?

Which type of vector is pBluescript?

Which outcome indicates that a colony on the pBluescript agar contains the recombinant plasmid?

Which is NOT true about enhancers sequences?

Which of the following are DNA binding motifs?

_______ hormones bind hormone response elements (HRE) in the nucleus to upregulate target genes.

Epigenetics (changes in chromatin structure) is heritable and irreversible.

Position-effect variegation is the change in gene expression caused by the translocation of genes between heterochromatin and euchromatin regions.

Which does NOT result from histone acetyltransferases (HATs) activity?

Which would lead to a decrease in gene expression?

What is the difference in dosage compensation of Drosophila and C. elegans?

Imprinted gene is the inheritance of parental acetylation pattern.

Which does NOT result in increased mRNA stability?

______ removes the hairpin loop of the Pre-miRNA to form ds siRNA.

G1/S checkpoint is controlled by [blank_start]Rb[blank_end]. DNA damage checkpoint is controlled by [blank_start]p53[blank_end].

Which is NOT correct regarding cell cycle control?

The p53 gene is mutated in >50% cancers.

Which gene is the most closely-associated with chronic myelogenous leukemia?

In Burkitt's lymphoma, ______ gene is translocated to a region controlled by a strong enhancer.

Which does NOT result in growth factor independent G1/S transition?

Which of the following cellular properties is the LEAST likely to cause cancer?

[blank_start]Ras[blank_end] hyperactivation results from a point mutation to Valine and GTP-independent activity. [blank_start]bcr-abl[blank_end] fusion protein is closely associated with chronic myelogenous leukaemia. [blank_start]HER2[blank_end] is an epidermal growth factor receptor, whose oncogenic properties arises from gene amplification. In Burkitt's lymphoma, [blank_start]c-myc[blank_end] gene is translocated to a region controlled by a strong enhancer. It is also amplified in childhood neuroblastomas.