Pregunta 1
Pregunta
What is the best way to assemble a genome?
Respuesta
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Have a reference gene to different species
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Have a reference gene to same species
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Have a reference genome to same species
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Have a reference genome to different species
Pregunta 2
Pregunta
Which is better DNA plat forms?
Respuesta
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Shorter reads cause of lower accurancy
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Longer reads easier to assemble
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Shorter reads cause of higher accurancy
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Longer reads harded to assemble
Pregunta 3
Pregunta
What happens next after you have fragments?
Pregunta 4
Pregunta
How do you increase the coverage for genome sequencing?
Pregunta 5
Pregunta 6
Pregunta 7
Pregunta
What is the function of genome scaffolds?
Pregunta 8
Pregunta
What step is after contig production when you are trying to build a gemone?
Respuesta
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Genome scaffolds with gaps in between contigs
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Join contigs using evidence from pair end data
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Place the contigs right next to eachother with no gaps
Pregunta 9
Pregunta
Why would you no fill the gap that is found between contigs on the genome scaffold?
Pregunta 10
Pregunta
What does N50 mean?
Respuesta
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The value of which 50% of nucleotides sequences are represented
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The percentage of gaps in the scaffold
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The number of genes produced
Pregunta 11
Pregunta
A k-mer are nucleotides that have been cut down to a certain size
Pregunta 12
Pregunta
What is the function of a contig?
Pregunta 13
Pregunta
How can you guess gap sizes on scaffold?
Pregunta 14
Pregunta
Long insert library
Respuesta
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4-40 kb library that has 100 bp on each end
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4-8 kb library that has 100 bp on each end
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4-40 kb library that has 10 bp on each end
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4-8 kb library that has 10 bp on each end
Pregunta 15
Pregunta
Why is De Novo genome assembly useful?
Respuesta
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Suitable for short coverage and long reads
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Suitable for long coverage and long reads
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Suitable for long coverage and short reads
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Suitable for short coverage and short reads
Pregunta 16
Pregunta
Why is the De Bruijn Graph useful?
Respuesta
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Suitable for high coverage and long reads
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Suitable for high coverage and short reads
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Suitable for short coverage and long reads
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Suitable for short coverage and short reads
Pregunta 17
Pregunta
How long do the k-mers need to be in overlap-layout-consensus?
Respuesta
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approx 24
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approx 2
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approx 240
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approx 2400
Pregunta 18
Pregunta
Why do the k mers in the De Bruijn model need to be similar sizes?
Respuesta
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If they are too small, it won't show overlaps
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If they are too long, it won't show overlaps
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If they are too small is easier to sequence
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If they are too long is easier to sequence
Pregunta 19
Pregunta
What is problem that arises when assembling fragments?
Pregunta 20
Pregunta
How do you solve the problem of repeats in genome assembly?
Respuesta
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Increase reading length which decreases sequence error rate
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Increase reading length which increases sequence error rate
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Decrease reading length which decreases sequence error rate
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Decrease reading length which increases sequence error rate
Pregunta 21
Pregunta
What takes up 45% of the genome?
Respuesta
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Large duplications
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Simple repeats
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Introns and Exons
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Transposons
Pregunta 22
Pregunta
Intergenic DNA have parasitic DNA that often fail to replicate properly
Pregunta 23
Pregunta
What is the name of the process whereby an exon from Gene A is placed in Gene B?
Respuesta
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Exon duplication
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Exon Shuffling
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Intron Duplication
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Intron Shuffling
Pregunta 24
Pregunta
What is the process whereby an exon is repeated and forms a new gene variant?
Respuesta
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Exon Duplication
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Exon Shuffling
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Intron Duplication
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Intron Shuffling
Pregunta 25
Pregunta
Entire gene families can be duplicated
Pregunta 26
Pregunta
What happens when entire gene super families get duplicated?
Pregunta 27
Pregunta
What happens when misaligned chromatids
Respuesta
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Repeats
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Transposons
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Exons
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Introns
Pregunta 28
Pregunta
Homologous genes in different organisms that encode proteins with the same function and have evolved by direct vertical descent
Respuesta
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Orthologous Genes
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Paralogous Genes
Pregunta 29
Pregunta
Homologous genes within an organism encoding proteins with related functions and often arise due to gene duplication
Respuesta
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Orthologous genes
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Paralogous genes