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DNA (labeling) for biochem and cell biology (lecture 2)

Descripción

1st year Biochemistry and molecular biology Test sobre DNA (labeling) for biochem and cell biology (lecture 2), creado por MrSujg el 29/11/2015.
MrSujg
Test por MrSujg, actualizado hace más de 1 año
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Resumen del Recurso

Pregunta 1

Pregunta
Label the image
Image:
97ba91f2-c354-451e-8038-fa8309d5ae4e (image/png)
Respuesta
  • 1'
  • 2'
  • 3'
  • 4'
  • 5'
  • alpha
  • Beta
  • gamma
  • base

Pregunta 2

Pregunta
What base is it ?
Image:
eaa093c2-a229-4330-ac20-3c1be45d3eb7 (image/png)
Respuesta
  • Adenine
  • Guanine
  • Cytosine
  • Thymine
  • Uracil

Pregunta 3

Pregunta
What base is it ?
Image:
4f7dbed1-80e3-4bcd-8896-0e7cc9065793 (image/png)
Respuesta
  • Guanine
  • Adenine
  • Cytosine
  • Thymine
  • Uracil

Pregunta 4

Pregunta
What base is it?
Image:
694ddb2d-0a6e-45fe-906f-f8558ee47aba (image/png)
Respuesta
  • Cytosine
  • Adenine
  • Guanine
  • Thymine
  • Uracil

Pregunta 5

Pregunta
What base is it?
Image:
a2296de7-40b2-4362-ab3f-98d6e43bed87 (image/png)
Respuesta
  • Thymine
  • Adenine
  • Uracil
  • Cytosine
  • Guanine

Pregunta 6

Pregunta
What base is it?
Image:
1da0c00c-fc24-42a5-8fa8-da2d5380d6b0 (image/png)
Respuesta
  • Uracil
  • Adenine
  • Guanine
  • Tymine
  • Cytosine

Pregunta 7

Pregunta
Identify primers
Image:
9d559227-f204-493c-96c5-c50babb61ca7 (image/png)
Respuesta
  • 3'
  • 5'

Pregunta 8

Pregunta
Label the image
Image:
6da2753f-30da-4d2c-a57c-539e77b339bf (image/png)
Respuesta
  • 5'
  • 3'
  • 5'
  • 3'
  • alpha
  • beta
  • gamma
  • O
  • C
  • S
  • H
  • N
  • F
  • DNA polymerase I
  • DNA polymerase II
  • DNA polymerase III

Pregunta 9

Pregunta
Label the image
Image:
32cffeda-7ef1-4b04-a973-2837895aebaf (image/png)
Respuesta
  • Minor groove
  • Major groove

Pregunta 10

Pregunta
Label the image
Image:
a142803c-4ca0-43b2-bbe1-3a6141b89c0f (image/png)
Respuesta
  • Guanine
  • Cytosine
  • Thymine
  • Adenine

Pregunta 11

Pregunta
Determine the primers
Image:
b1f5e341-d54a-4dd3-9f52-ba75d4dbfee8 (image/png)
Respuesta
  • 5'
  • 3'
  • 3'
  • 5'
  • 3'
  • 5'
  • 5'
  • 3'

Pregunta 12

Pregunta
Label the image
Image:
aa762e2c-7cbe-4134-82bc-6577de8f9f51 (image/png)
Respuesta
  • A-DNA
  • B-DNA
  • Z-DNA

Pregunta 13

Pregunta
Label the image
Image:
3898de21-03d4-46d8-80c0-277b8e37b6f7 (image/png)
Respuesta
  • Conservative mechanisms
  • Semiconservative mechanism

Pregunta 14

Pregunta
Label the image
Image:
98aa9ee9-9028-456d-8c43-fc2bf06f14f5 (image/png)
Respuesta
  • Origin
  • Growing fork

Pregunta 15

Pregunta
Label the image
Image:
354ecee5-baa7-461d-a1ef-374333359b3a (image/png)
Respuesta
  • EcorRI restriction site
  • Origin
  • Replication bubble
  • Time of replication

Pregunta 16

Pregunta
Labelt the replication cartoon
Image:
c7a69787-e289-42b7-80ed-2801a3fa509c (image/png)
Respuesta
  • Primase
  • SIngle0stranded region of parent DNA
  • RNA primer
  • DNA polymerase III
  • Newly made DNA
  • DNA polymerase I
  • RNA still to be replaced
  • Newest DNA
  • Completed daughter cell

Pregunta 17

Pregunta
Label the cartoon showing the unwinding at the origin
Image:
f63fc1cf-bad8-47b6-a967-a46d3921a33c (image/png)
Respuesta
  • 9-mers
  • Negatively supercoiled template
  • DnaA
  • ATP
  • Initial complex
  • Open complex
  • DnaC
  • DnaB (helicase)
  • Prepriming complex

Pregunta 18

Pregunta
Label the cartoon showing Simultaneous synthesis on both strands
Image:
9ef4a3c0-4be0-419c-823c-5b4b55e09987 (image/png)
Respuesta
  • Point of joining
  • Lagging strand
  • Okazaki fragment
  • Short RNA primer
  • Leading strand
  • Parental DNA duplex
  • Daughter duplex
  • Direction of fork movement

Pregunta 19

Pregunta
Label the cartoon showing the participitation of topoisomers in DNA unwinding
Image:
a577fc9f-ba3c-4bac-9498-9daff7642b2b (image/png)
Respuesta
  • Covalently closed circular template
  • DNA replication
  • Unwound parental duplex
  • Negative supercoils
  • Positive supercoils
  • Overwound region

Pregunta 20

Pregunta
Label DNA polymerase
Image:
35a86cb2-ac0d-4e63-9240-5612df9d0131 (image/png)
Respuesta
  • Fingers
  • Thumb
  • Growing strand
  • Template strand
  • Exo
  • Palm
  • Pol

Pregunta 21

Pregunta
Label the image showing mismatch repair
Image:
c4c569cb-23b4-438f-a8ae-961efeca3a5f (image/png)
Respuesta
  • Daughter strand
  • Parental strand
  • Mismatched base pair
  • Mismatch recognition
  • MutS
  • MutH
  • MutH cleavage
  • Gap repair by DNA polymerase and ligase

Pregunta 22

Pregunta
Label the cartoon showing excision repair
Image:
5fb753e5-d05a-476d-bb81-942d4ad07d81 (image/png)
Respuesta
  • Initial damage recognition
  • 23B
  • XP-C
  • Opening of DNA double helix
  • TFIIH
  • XP-G
  • RPA
  • XP-F and XP-G endonucleases
  • DNA polymerase and DNA ligase

Pregunta 23

Pregunta
Label the image showing PCR
Image:
29516573-140e-440c-b28f-033e27392edf (image/png)
Respuesta
  • DNA Denaturation, annealing of primers
  • Elongation of primers
  • DNA denaturation; annealing or primers 2
  • Elongation of primers 2

Pregunta 24

Pregunta
Label the image
Image:
7889a418-6e28-4396-acae-ecd035ba5303 (image/png)
Respuesta
  • RNA polymerase I
  • RNA polymerase II
  • RNA polymerase 3

Pregunta 25

Pregunta
Label the image showing the Transcription in Prokaryotes
Image:
008be8d7-b8c0-4757-8426-80cd106a4f30 (image/png)
Respuesta
  • Promoter DNA
  • -35 sequence
  • 10 sequence
  • Coding strand
  • Template strand
  • Startpoint

Pregunta 26

Pregunta
Labelt the image showing A-Binding Domains (DBDs)
Image:
6ce4192a-816c-4de6-83dd-d1e7029bcbc3 (image/png)
Respuesta
  • Helix-turn-helix motif
  • The lambda repressor
  • Zinc finger motif
  • Leucine zipper motif
  • Helix-loop-helix motif

Pregunta 27

Pregunta
Label the image showing the lac operon activation
Image:
a17d1ab7-041c-4644-b4cc-2a54a72b78d2 (image/png)
Respuesta
  • Beta-galactosidase
  • RNA polymerase bound to promoter
  • lac-mRNA
  • Lactose
  • RNA polymerase transcribing the operon
  • lacI gene
  • Inactive form of repressor

Pregunta 28

Pregunta
Label the image showing the regulation of the lac Operon
Image:
c5c2f9ba-30ba-4412-89bd-33b192cd65e6 (image/png)
Respuesta
  • Promoter
  • CAP site
  • CAP
  • a70-Pol
  • lac repressor
  • Lactose
  • cAMP

Pregunta 29

Pregunta
Label the image showing Eukaryotic promoters
Image:
3e346027-d6c6-4e0c-9b13-28fb2881070c (image/png)
Respuesta
  • GC box
  • CAAT box
  • TATA box
  • Proximal control elements
  • Core promoter
  • Start of transcription
  • Poly (A) site
  • 5' untranslated region (leader)
  • 3' untranslated region (trailer)

Pregunta 30

Pregunta
label the image showing General Eukaryotic Transcription Factors
Image:
4d83b5f1-bcbd-4cfe-946f-73ad4570814b (image/png)
Respuesta
  • Core promoter
  • Startpoint
  • TFIID
  • TFIIA
  • TFIIB
  • TFIIF
  • RNA polymerase 2
  • TFIIE
  • TFIIH
  • Transcription begins

Pregunta 31

Pregunta
Label the image showing Additional Eukaryotic Transcription Factors
Image:
0ddef686-0faa-4bfd-a357-fa0cfb066fe2 (image/png)
Respuesta
  • Enhancer
  • Core promotor
  • TATA
  • Inr
  • Gene
  • Activators
  • Coactivators
  • Enhanceosome
  • TFIID
  • Activator and Coactivator
  • Transcription

Pregunta 32

Pregunta
label the image showing Tissue-Specific Gene Expression
Image:
83151982-9958-4315-afd5-6c724231c60c (image/png)
Respuesta
  • Control elements
  • Core promoter
  • Regulatory transcription factors
  • General transcription factors
  • RNA polymerase
  • Transcription complex
  • DNA
  • RNA transcript

Pregunta 33

Pregunta
Label the image showing how the final mRNA is made
Image:
c354a8e3-273d-416f-8c0c-774c4679b0d0 (image/png)
Respuesta
  • Exon
  • Intron
  • Poly (A) site
  • Termination sites
  • Transcription 5' capping
  • Cleavage at poly (A) site
  • Polyadenylation
  • RNA splicing

Pregunta 34

Pregunta
Labe the image showing Microarrays: Genome-Wide Analysis
Image:
4c14d5c9-3afe-4542-bb21-0025b83b3070 (image/png)
Respuesta
  • RNA isolation
  • Reverse transcriptase labeling
  • Combine targets
  • Hybridaze to microarray

Pregunta 35

Pregunta
Label the image showing the molecular Dogma
Image:
3d5f7294-4159-45cc-9661-7116bc7deb07 (image/png)
Respuesta
  • Transcription
  • Translation
  • Assembly
  • tRNA
  • rRNA
  • Ribosomes
  • Polypeptides
  • Proteins
  • Ribosomal proteins

Pregunta 36

Pregunta
Label the image
Image:
188e25d1-ef20-4a80-a195-dab16bcb2a5c (image/png)
Respuesta
  • Primary RNA transcript
  • 5'
  • 3'
  • DNA
  • Promoter
  • Transcription
  • Coding sequence
  • Upstream
  • Downstream

Pregunta 37

Pregunta
Follow the genetic code to match the appropriate amino acid to each codon
Image:
9f0c4d2f-c570-4736-8759-460cdfacd987 (image/png)
Respuesta
  • Ala
  • Cys
  • Leu
  • Arg
  • Ile
  • Val
  • Tyr
  • Glu

Pregunta 38

Pregunta
Label the type of the mutation
Image:
aad2b58a-2125-4ddb-8f29-d7fa6f906d09 (image/png)
Respuesta
  • Frameshift
  • Missense
  • Nonsense

Pregunta 39

Pregunta
Identify the type of ribosome, and the size of the assembled unit
Image:
b441155e-7692-40b4-af8a-d2d1d07be6fd (image/png)
Respuesta
  • Prokaryotic
  • Eukaryotic (vertebrate)
  • 70S
  • 80S

Pregunta 40

Pregunta
Label the image showing aminoacyl-tRNAsynthetases
Image:
baa4b803-cf3e-4ad9-9e39-48755e355485 (image/png)
Respuesta
  • High-energy ester bond
  • Amino acid (Phe)
  • mRNA
  • Aminoacyl-tRNA
  • tRNA specific for Phe

Pregunta 41

Pregunta
Label the image
Image:
080fb1c4-1028-4c23-ba9c-9f2f04bdeeb3 (image/png)
Respuesta
  • Adenosine deaminase
  • Adenosine
  • Inosine

Pregunta 42

Pregunta
Label the initiator methionine
Image:
3d29c23b-2174-46e0-b136-635273233ac0 (image/png)
Respuesta
  • Formyl group
  • Carboxyl group
  • Acetyl group
  • Phosphate group

Pregunta 43

Pregunta
Label each mRNA
Image:
bcc16fb4-5a72-4b5d-be77-3183db133ab6 (image/png)
Respuesta
  • Prokaryotic mRNA
  • Eukaryotic mRNA

Pregunta 44

Pregunta
Label the ribosome sites
Image:
a6e21ea5-1b72-4ce0-8d08-b98dfdcec962 (image/png)
Respuesta
  • A site
  • P site
  • E-site
  • mRNA-binding site
  • mRNA
  • Growing peptide chain

Pregunta 45

Pregunta
Label the image showing translation
Image:
5b815481-de9a-46a3-a414-fbdc881e5324 (image/png)
Respuesta
  • P site
  • E site
  • A site
  • Release factor
  • free tRNA
  • Polypeptide
  • Ribosomal subunits
  • mRNA

Pregunta 46

Pregunta
Label the image showing translation mechanism
Image:
b6e85445-9c31-41bd-9cdd-7706e8013886 (image/png)
Respuesta
  • AUG start codon
  • UAG stop codon
  • mRNA
  • Initiation
  • Elongation
  • Termination
  • Release factor
  • Completed polypeptide
  • Recycling of translational components
  • Ribosomal subunits

Pregunta 47

Pregunta
Label the image showing western blotting
Image:
4753de13-785f-4e6c-bd4e-468584298cc8 (image/png)
Respuesta
  • Electrophoresis and transfer
  • Antibody detection
  • Chromogenic detection
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