441574
Descripción
Test por gina_evans0312, actualizado hace más de 1 año
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Creado por gina_evans0312
hace casi 11 años
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Pregunta 1
Pregunta
DNA helicase unwinds DNA into ssDNA for what processes?
Respuesta
-
DNA repair
-
DNA replication
-
DNA recombination
-
DNA destruction
Pregunta 2
Pregunta
DNA helicase's process and translocate branched structures
Respuesta
- True
- False
Pregunta 3
Pregunta
Name (a) branched DNA structure(s)
Respuesta
-
Holliday junctions
-
D loops
-
Binary helix
-
T loops
Pregunta 4
Pregunta
RNA helicases cannot destabilize RNA secondary structures
Respuesta
- True
- False
Pregunta 5
Pregunta
RNA helicases help in which of the following processes
A- Promote ribosome assembly
B- RNA translocation
C-RNA splicing
D- RNA editing and transport
E-RNA degredation
Respuesta
-
A, C & D
-
B, C & E
-
A only
-
E only
-
All of them
Pregunta 6
Pregunta
Some helicases can work on both RNA & DNA
Respuesta
- True
- False
Pregunta 7
Pregunta
Which of the following unwind RNA-DNA hybrids
Respuesta
-
RNA helicase, then DNA helicase
-
DNA helicase, then RNA helicase
-
RNA/DNA helicases
Pregunta 8
Pregunta
RNA/DNA helicases perform which of the following functions?
Respuesta
-
Transcription termination
-
Regulation of DNA replication initiation
-
RNA degredation
Pregunta 9
Pregunta
All helicases use energy from NTP hydrolysis to translocate along polynucleotides
Respuesta
- True
- False
Pregunta 10
Pregunta
Helicases are dependent on Fe2+
Respuesta
- True
- False
Pregunta 11
Pregunta
Helicases can take which of the following forms (commonly)
Respuesta
-
Hexamers
-
Monomers
-
Trimers
-
Dimers
-
Tetramers
-
Pentamers
Pregunta 12
Pregunta
Helicases can have an oligomeric structure
Respuesta
- True
- False
Pregunta 13
Pregunta
Helicases can be
Respuesta
-
Unidirectional
-
Bidirectional
-
Both
-
Bidirectional or unidirectional
Pregunta 14
Pregunta
What is the difference between translocases and helicases?
Respuesta
-
Translocases -transcribe and separate DNA strands Helicases- transcribed DNA strands only
-
Helicases-transcribe and separate DNA strands Translocases- transcribed DNA strands only
Pregunta 15
Pregunta
Give an example of a DNA/RNA unwinding helicase/translocase
Respuesta
-
PCRA
-
Type 1 restriction enzymes
-
Ruv B/Reg G
-
Mfd
Pregunta 16
Pregunta
Name a helicase/translocase that effects protein motion on DNA
Respuesta
-
PCRA
-
Type 1 Restriction Enzyme
-
Ruv B/ Reg G
-
Mfd
Pregunta 17
Pregunta
Name a necleoprotein complex remodelling helicase
Respuesta
-
Mfd
-
Ruv B/Reg G
-
PCRA
-
Type 1 Restriction Enzymes
Pregunta 18
Pregunta
Name a DNA/RNA remodelling helicase
Respuesta
-
Ruv B/ Reg G
-
PCRA
-
Type 1 Restriction Enzymes
-
Mfd
Pregunta 19
Pregunta
Helicases are divided into seven superfamilies based on primary structure, and then 3 structural classes
Respuesta
- True
- False
Pregunta 20
Pregunta
The two structural classes are monomers and dimers
Respuesta
- True
- False
Pregunta 21
Pregunta
All helicases contain a Rec B fold
Respuesta
- True
- False
Pregunta 22
Pregunta
EVERY helicase molecule contains which of the following?
Respuesta
-
An ATP binding site
-
An NTP binding site
-
A separate polynucleotide binding site
-
A GTP binding site
-
A linked polynucleotide binding site
Pregunta 23
Pregunta
The _TP binding site and the nucleotide binding site on a helicase are allosterically linked
Respuesta
- True
- False
Pregunta 24
Pregunta
Type A helicases (like PCRA) travel in which direction?
Respuesta
-
3' > 5'
-
5' > 3'
-
Both ways
Pregunta 25
Pregunta
Rec D is a Type 2 helicase- in which direction does it travel?
Respuesta
-
5' > 3'
-
Both ways
-
3' . 5'
Pregunta 26
Pregunta
Rec BCD is a bipolar helicase
Respuesta
- True
- False
Pregunta 27
Pregunta
In a bacterial Holliday Junction with a stalled replication fork, what is required to allow replication to continue?
Respuesta
-
RuvB
-
ATP
-
PRCA
-
GTP
Pregunta 28
Pregunta
If Holliday Junctions cannot be repaired, they lead to a cruciform structure
Respuesta
- True
- False
Pregunta 29
Pregunta
What is the slowest rate of movement of a helicase?
Respuesta
-
10 bp/sec
-
8 bp/sec
-
12 bp/sec
Pregunta 30
Pregunta
Rate of helicase movement is dramatically dependent on protein partners
Respuesta
- True
- False
Pregunta 31
Pregunta
The fastest known helicase moves around 4000 bp/sec
Respuesta
- True
- False
Pregunta 32
Pregunta
Define Helicase Processivity
Respuesta
-
How fast it moves along the DNA/RNA
-
How far it can move before it falls off
-
How far it moves per substrate hydrolysis
Pregunta 33
Pregunta
Helicases can move around 10-30,000 base pairs before falling off depending on the helicase (and it's protein partners)
Respuesta
- True
- False
Pregunta 34
Pregunta
Step size is a theoretical limit due to the entropy of NTP hydrolysis
Respuesta
- True
- False
Pregunta 35
Pregunta
Step size is the theoretical avg no of bp unwound per NTP hydrolysis cycle
Respuesta
- True
- False
Pregunta 36
Pregunta
The limit on step size = Energy required to melt the bp x step size
Respuesta
- True
- False
Pregunta 37
Pregunta
What is the experimental limit of step size?
Respuesta
-
0.3-3 bp
-
0.5- 5bp
-
0.7- 7 bp
Pregunta 38
Respuesta
-
The 'Inchworm' helicase movement model
-
The 'active rolling model'
Pregunta 39
Pregunta
Which of the following is believed to be the unwinding mechanism used by helicases?
Respuesta
-
Passive- DNA is frayed by heat and the translocation occurs in the presence of ATP
-
Active- ATP activates molecules that bind to and actively distort DNA before ATP dependent translocation can occur
Pregunta 40
Pregunta
PCRA is made of how many domains?
Respuesta
-
4 - 1A/B & 2A/B
-
2 - 1A & 2A
-
6 1A/B, 2A/B & 3A/B
Pregunta 41
Pregunta
PCRA activity is dependent on its protein partners
Respuesta
- True
- False
Pregunta 42
Pregunta
PCRA is an ATPase with...
Respuesta
-
A 3' > 5' polarity
-
A 5' > 3' polarity
-
A low rate of 30 bp/sec
-
A high rate of 300 bp/sec
-
A low processivity of 10 bp/sec
-
A high processivity of 100 bp/sec
-
A step size of 16 bp/ATP
-
A step size of 6 bp/ATP
Pregunta 43
Pregunta
The conserved Helicase motifs of PCRA are in the accessory domains
Respuesta
- True
- False
Pregunta 44
Pregunta
Aromatic stacking occurs between the inserted aa and bp
Respuesta
- True
- False
Pregunta 45
Pregunta
Which of the following will bind to the DNA
Respuesta
-
Tryptophan
-
Phenylalanine
-
Tyrosine
-
Histidine
Pregunta 46
Pregunta
The complex of PCRA-DNA remains the same during ATP binding and ATP hydrolysis
Respuesta
- True
- False
Pregunta 47
Pregunta
Duplex distortion in the PCRA substrate complex suggests a Passive unwinding system
Respuesta
- True
- False
Pregunta 48
Pregunta
Partner proteins are required by PCRA for which processes?
Respuesta
-
Loading
-
Unwinding
-
Activation
-
Catalytic modification
Pregunta 49
Pregunta
Hexameric helicases can be part of the replisome
Respuesta
- True
- False
Pregunta 50
Pregunta
Hexameric helicases have a toroidal strcture to confer high processivity
Respuesta
- True
- False
Pregunta 51
Pregunta
ATP binds where on hexameric helicases?
Respuesta
-
To the pockets present in each monomer
-
To the pocket made by the interaction of all 6 monomers
Pregunta 52
Pregunta
ssDNA is bound in the central channel to a 'spiral staircase' of bining loops
Respuesta
- True
- False
Pregunta 53
Pregunta
ATP binding pocket changes _ around the ring
Respuesta
-
Sequentially- one at a time
-
At the same time
-
Sequentially- two at a time
Pregunta 54
Pregunta
ATP binding correlates with loop hight in the central channel
Respuesta
- True
- False
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