446901
Pregunta 1
Pregunta
What is a genome?
Respuesta
-
It is all of the genes possessed by an individual organisms.This includes the sequence of bases in the entire DNA of an organism.
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It is the sequencing of an organism.
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It is a section of the gene possessed by an organism.
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This is all the genes possessed by all of the organisms in a similar species.
Pregunta 2
Pregunta
What are the advantages of genome sequencing?
Respuesta
-
Shows evolutionary relationships.
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Shows the effects of mutations.
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Shows the possibility of a mutation.
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Shows the alleles for a disease.
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Shows the importance of bases in DNA.
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Shows how genes can be manipulated for human benefit.
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Helps create bio-fortified products.
Pregunta 3
Pregunta
'Micro-satellites are long sequences of DNA'.
Respuesta
- True
- False
Pregunta 4
Pregunta
'BACs stands for Bacterial Artificial Chromosomes which are man-made plasmids'.
Respuesta
- True
- False
Pregunta 5
Pregunta
Why are computer programmes used in genome sequencing?
Respuesta
-
To compare the overlapping regions of DNA.
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To check the criminals of the world.
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To compare whether the genes can be bio-fortified.
Pregunta 6
Pregunta
What is genetic fingerprinting?
Respuesta
-
DNA fragmentation and electrophoresis gives banding patterns unique to individuals.
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It gives the sequence of DNA.
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It obtains a particular gene and inserted into a recipient's organism.
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Comparing genes for the same proteins across a range of organisms.
Pregunta 7
Pregunta
What is recombinant DNA?
Respuesta
-
It is made by cutting DNA and sticking it together.
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This occurs by conjugation of bacteria which binds two forms of plasmids.
Pregunta 8
Pregunta
'Restriction enzymes restrict the DNA from transcribing mRNA'.
Respuesta
- True
- False
Pregunta 9
Pregunta
Why is DNA heated in automated sequencing?
Respuesta
-
To break the hydrogen bonds (by denaturing them) which gives two polynucleotide chains.
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To create a number of bases that can be amplified by PCR.
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To separate the fragments for electrophoresis.
Pregunta 10
Pregunta
Why do the DNA fragments in electrophoresis move to the anode (positively charged electrode)?
Respuesta
-
This is because they are positively charged and two positive separates them.
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This is because they are negatively charged and the negative DNA fragments are attracted to the anode.
Pregunta 11
Pregunta
Why is buffer solution required in electrophoresis?
Respuesta
-
To separate the sections of DNA.
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To conduct electricity.
Pregunta 12
Pregunta
'The longer the fragments, the quicker they move' in electrophoresis.
Respuesta
- True
- False
Pregunta 13
Pregunta
Primers bind at the complementary regions of DNA and probes bind at the start of DNA.
Respuesta
- True
- False
Pregunta 14
Pregunta
Annealing is binding of base sequences.
Respuesta
- True
- False
Pregunta 15
Pregunta
What are sticky ends?
Respuesta
-
They are short, unpaired, exposed bases.
-
They are bases that are attached to restriction enzymes.
Pregunta 16
Pregunta
The insulin comes from beta cells in the liver.
Respuesta
- True
- False
Pregunta 17
Pregunta
How is the DNA formed from the insulin gene?
Respuesta
-
mRNA is extracted and incubated via reverse transcriptase which makes DNA using RNA.
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It is completed by conjuration of bacteria formed in fermenters.
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It is completed by the ligase enzyme.
Pregunta 18
Pregunta
Why is the ligase enzyme used to make insulin?
Respuesta
-
It combines the insulin DNA and the plasmid from the bacteria (usually E. Coli).
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Creates the backbone of DNA.
Pregunta 19
Pregunta
Golden rice is bio-fortified with vitamin B.
Respuesta
- True
- False
Pregunta 20
Pregunta
Where is beta carotene extracted from?
Respuesta
-
Daffodils
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Bacterium: Erwina uredonma
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Lillies
-
Wheat
Pregunta 21
Pregunta
Xenotransplantation is the transplantation of cells, tissues or organs between animals of different species.
Respuesta
- True
- False
Pregunta 22
Pregunta
Gene therapy involves replacing a gene that are not functioning.
Respuesta
- True
- False
Pregunta 23
Pregunta
Somatic gene therapy only affects skin cells.
Respuesta
- True
- False
Pregunta 24
Pregunta
Genes are passed onto offspring in germ line gene therapy.
Respuesta
- True
- False
Pregunta 25
Pregunta
What are the ethical effects of microorganisms?
Respuesta
-
Cross contamination of other microorganisms.
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Mutations of microorganisms which could lead to resistance.
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Produce deadly toxins that lay in our food.
Pregunta 26
Pregunta
What are the temperatures of PCR that need to be set?
Respuesta
-
95, 55 and 72.
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75, 80 and 40.
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60, 40 and 81.
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110, 80 and 41.
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