DISAGGREGATION OF EXPLANT

Nodos de los diagramas

• DISAGGREGATION OF EXPLANTS

• FINE DISSECTION

• MECHANICAL DISAGGREGATION

• COLD TRYPSIN

• WARM TRYPSIN

• COLLAGENASE

• DISPERSED PRIMARY CULTUTURE

• SUBCULTURE

• PRIMARY EXPLANT

• TRANSFER

• 2ND EXPLANT

• OVER NIGHT INCUBATION

• LONG INCUBATION

• CENTRIFUGE

• CELL LINE

• OUTGROWTH

• RESUSPEND  AND SEED