Microbiology II

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Fichas sobre Microbiology II, creado por J yadonknow el 21/01/2018.
J yadonknow
Fichas por J yadonknow, actualizado hace más de 1 año
J yadonknow
Creado por J yadonknow hace casi 7 años
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What is the size range of prokaryotes? 0.2um to >700um
What is the size range of eukaryotic cells? 10um to >200um
What are the advantages of being small? Large SA:V Faster nutrient exchange per unit cell volume Grow faster Can support a large population Faster evolution more cellular divisions = higher rate of mutations within a population Allows rapid adaptation to changing environments
What are the limitations to being small? Must fit all essential organelles and materials inside 0.15um diameter to fit all organelles Anything less than 0.1um is unlikely to be a cell
Describe briefly the process of gram staining CV Iodine Ludol's reagant saffranin
What are endospores? Highly differential cells that are resistant to heat/harsh chemicals/radiation Survival structures
Describe the process of sporulation Essential nutrient is exhausted Vegetative cell stops growing Endospore develops within the vegetative cell and is released as a mature endospore Germinates when conditions are favourable to form a vegetative cell
What names relate to the differing stages of endospore development within a vegetative cell? Terminal Sub-terminal central
What is the structure of an endospore? (5) Many layers = protection Exosporium - thin protein covering Spore coat - layers of spore specific proteins Cortex - loose cross-linked murein Core - everything else
Function of fimbriae (2) Let cells adhere to surfaces and each other Assist in disease process
Function of pili (2) Conjugation Adhesion of pathogen to specific host tissues
How to visualise pili E microscope coated in virus particles
What is polar flagellation? Flagella attached to one or both ends of an MO
What is tuft flagellation? Group of flagella attached to one end type of polar
What is peritrichous flagellation? Multiple locations
Describe flagella Helical filaments composed of many flagellin copies, wavelengths are characteristic of species
What is the motor? The central rod embedded in the cell membrane that passes through a series of rings, with Mot proteins acting as stators
Where does the energy for rotation come from? Movement of H+ across membrane through mot complex
MOA (3) H+ flow -> channel Exert ES<3 on helically arranged charges of ring <3 btw. charges causes rotation
Describe the movement control of peritrichous cells CCW rotation Tumbles when switching to CW, flagella push apart Flagella bundle and rotate CCW
Methods of polar movement (2) Reversible flagella rotation Unidirectional rotation
Describe gliding PS slime used to connect cell surf w/ solid surf. As slime adheres to surface cell pulled along
Which pili facilitate this type of movement? Type IV twitching motility pili
What is the life cycle of myxobacteria? (5) Sporangioles Myxospores Aggregation of veg cells Mound Fruiting body
MOA (6) 1. Sporangioles release myxospores 2. Myxospores germinate to veg cells 3. Veg cells swarm, pick up each other's slime trails to form aggregates 4. Agg. heap to form mound 5. Mounds of MXB diff. into mature fruiting body 6. Myxospores produced w/in sporangioles
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