transgenic animals

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(molecular theraputics) Molecular Theraputics Fichas sobre transgenic animals, creado por lumen7 el 20/04/2013.
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xenozoonoses diseases that can be passed from animals, whether wild or domesticated, to humans
Embryonic Stem cell-mediated gene transfer 1.isolate stem cells from embryo 2. insert desired gene into cells 3.Cells containing the desired DNA are incorporated into the host’s embryo, resulting in a chimeric animal 3.test for transgenes 4.reconstructed oocyte is activated to initiate development 6. reconstructed embryo can result in a viable offspring (2–3% of time)
DNA microinjection to create transgenic animals 1. ds DNA components of the transgene are combined enzymatically to yield a transgene expression cassette 2. Transgene cassettes are inserted into plasmid vectors and cloned 3. Transgene-bearing plasmids are transfected into cultured eukaryotic cells to evaluate expression of the transgene 4. Plasmid-free transgene fragments are introduced directly into embryonic pronuclei 5. Manipulated embryos are placed in the reproductive tract of a pseudopregnant recipient 6. The genomic DNA of live-born pups is analyzed for the presence of the transgene DNA sequence
transgenic animal carries a foreign gene that has been deliberately inserted into its genome or has its gene deleted
animal model living animal used during the research and investigation of human diseases
animal cloning the production of genetically identical animals
how is a functional protien produced in a transgenic animal? 1.inserted gene expressed in mammary glands 2.The post-translationally modified protein isolated from the milk 3.If protein is similar to human it can be developed as a pharmaceutical.
Transgenic sheep and pigs have been created that produce ... human blood clotting factors (VIII and IX) in their milk.
Transgenic rabbits have been created that produce..... human interleukin-2.
95% of transgenic animals used in biomedical research are... mice
human products produced by transgenic animals 1. anti-thrombin III 2. Collagen 3.Fibrinogen 4.fertility hormone 5.hemoglobin 6.serum albumin 7.Lactoferrin 8.Tissue plasminogen activator
A transgenic animal for pharmaceutical production should: 1.Produce the desired drug at high levels w/o endangering its own health 2.Pass its ability to produce the drug at high levels to its offspring
Strategy for production in mammary glands 1.Couple gene for the protein drug with signal directing production in the mammary gland 2.gene present every cell but functional only in mammary gland 3.protein drug made only in milk
3 Gene Transfer Methods 1Microinjection of cells (oocytes) 2Embryonic stem cell-mediated 3Retrovirus-mediated
animal cloning DNA of an oocyte is removed and replaced by that of the donor cell. multiple, genetically identical individuals can be made from a donor cell source (an individual animal or fetus)
how to make a cybrid 1.take whole human cell or DNA and put into empty animal egg 2. egg divides and is 99.9% human and 0.1% animal
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