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Creado por gina_evans0312
hace más de 10 años
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Pregunta | Respuesta |
Initial Correlation- Cervical Cancer Found In | Married/widowed women and prostitutes |
Initial Correlation- Cervical Cancer Absent In | Nuns |
Initial Correlation- STIs? | No correlation found with a particular STI and cervical cancer |
Initial Correlation- Why not HPV 11? | No known correlation |
HPV11 Fishing Tool | HPV11 is part of suspected HPV family- so if a HPV strain is involved, it will have a similar genome |
DNA Fishing Done By | Southern Blotting |
Low Stringency | Washing to remove probes done in conditions that allow poorly matched probes to remain bound |
High stringency | Washing done in conditions that only allow perfectly matched probes to remain bound |
Comparing Low and High Stringency | A member of the HPV family would bind with HPV11 at low stringency, but not at high |
HPV11 Fishing Results | Weak binding even at low stringency- not HPV11 but of the HPV11 family |
How was HPV16 found? | A genomic library- used a nitrocellulose filter to find colonies with HPV DNA to use as a probe |
Use of Found HPV as a probe | Isolated HPV DNA from samples made into a plasmid for probing |
Repeat of Southern Blotting | Even under very high stringency, very clear binding- then comapred against all samples, all contained new HPV strain |
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