PCR (lab notes)

Pregunta	Respuesta
Alu insertion?	It's 300 bp long and it is a pice of mobile DNA which has the ability to copy and move within genome
What are the 3 PCR Steps?	1) Denaturation 2) Annealing 3) Extension
Briefly explain the 3 PCR steps?	1) Denaturation (90-92 C); two strand of DNA helix 2) Annealing (50-60 C); 2 primers to attach to both sides of DNA (aka DNA template) 3) Extension (70-74 C): Synthesis of complementary strands
What are the possible PCR results with a section of cur 16 around the Alu insertion?	1) chr with insertion have PCR product = 941bp 2) chr without insertion have PCR product = 641 bp 3) Two homozygous, 1 or the other 4) 1 heterozygous have both lengths of product
Mutation in introns	If there is a mutation in an intron (non coding region DNA) then it does not cause a change in protein and which would be the opposite in if there is a mutation in the exon

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	Creado por enrique.salanic hace más de 10 años