128531
Descripción
Mapa Mental por charelle12, actualizado hace más de 1 año
|
Creado por charelle12
hace más de 11 años
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|
DNA Technology
- Polymerase Chain Reaction
- amplifies the
amount of a
DNA sample
- 1) DNA
heated to 95
to break H2
bonds
- 2) Cool to 35 to
primers bind to
start of each
strand, prevent
rejoining + act as
marker for
polymerase
- 3) heated to 73
+ thermostable
TAQ
polymerase
replicates
strand
- each time
process
repeats,
DNA
doubles
- each time
process
repeats,
DNA
doubles
- 3) heated to 73
+ thermostable
TAQ
polymerase
replicates
strand
- 2) Cool to 35 to
primers bind to
start of each
strand, prevent
rejoining + act as
marker for
polymerase
- 1) DNA
heated to 95
to break H2
bonds
- DNA probe
- locates section of
DNA, consists of short
lengths of single
stranded DNA with
specific nucleotide
sequence. To dectect-
radioactive/fluorescent
for xray/laser scanner
- DNA profiling ( fingerprinting)
- 1) DNA cut
with restriction
endonucleases
into fragments
- 2) Fragments
seperated by gel
electrophoresis
due to bands,
smaller fragments
move further
- 3) Bonds heated to
be single stranded,
transferred onto
nylon and washed
with DNA probes
complementary to
MRS's
- 4) Repeated
with several
probes for
different
MRS's +
fragments
detected
- 5) DNA bound
to probe show
up, bands are
fingerprints
- 5) DNA bound
to probe show
up, bands are
fingerprints
- 4) Repeated
with several
probes for
different
MRS's +
fragments
detected
- 3) Bonds heated to
be single stranded,
transferred onto
nylon and washed
with DNA probes
complementary to
MRS's
- 2) Fragments
seperated by gel
electrophoresis
due to bands,
smaller fragments
move further
- 1) DNA cut
with restriction
endonucleases
into fragments
- locates section of
DNA, consists of short
lengths of single
stranded DNA with
specific nucleotide
sequence. To dectect-
radioactive/fluorescent
for xray/laser scanner
- amplifies the
amount of a
DNA sample
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