T-Cell Deficiencies

1. Infections
   1. Viruses

      Nota:

      • egCMV,
   2. Fungi

      Nota:

      • egPCP
   3. Protozoa
   4. IC bacteria

      Nota:

      • egTB

1. FBC

   Nota:

   • lymphocytes<2x10^9
2. IG levels in Immunology labs

   Nota:

   • Low/Absent IgG may have IgG from mom    
3. lymphocyte subsets

   Nota:

   • #&% T-cellsB-cellsNK
4. Expression of proteins

   Nota:

   • eg.Common γ chain    
5. Molecular Investigation

   Nota:

   • confirm genetic defect    
6. Biochemistry

   Nota:

   • eg.ADA Def - check for abnormal metabolites, dATP    

Nota:

• severecombinedimmunodeficiency

1. Common Y chain

   Nota:

   • 50%-xlinked
   • -+-
   1. diagnosis

      Nota:

      • Stain and detect by flow cytometry Western blot  downstream events -eg. JAK3, tyrosine phosphorylation)    
2. IL7-Rα

   Nota:

   • 1-2%
   • -++
3. Jak 3 Kinase

   Nota:

   • 10%
   • -+-
4. RAG1/2

   Nota:

   •     --+   
5. ADA
   1. --+
6. T+