481275
Paper Chromatography
- What is paper chromatography?
- is a technique of using a
solvent to separate a mixture
into its components for
identification purposes and
determining of purity.
- very useful method to separate and
identify components in a sample like
dyes in inks, poison in drugs etc.
- very useful method to separate and
identify components in a sample like
dyes in inks, poison in drugs etc.
- is a technique of using a
solvent to separate a mixture
into its components for
identification purposes and
determining of purity.
- Process: 1.a pencil line
is drawn across the
chromatography paper
to indicate the starting
position of mixture
spots.
- 2. important to use
a pencil because
inks will sumuge
- 3. Paper dipped into a solvent
- mixture dissolve in the solvent and moves up the paper
- mixture dissolve in the solvent and moves up the paper
- 3. Paper dipped into a solvent
- results:
- 2. important to use
a pencil because
inks will sumuge
- How does it work?
- http://www.youtube.com/watch?v=PN5YiY0Wm6o
- http://www.youtube.com/watch?v=gpFb635N2wo
- http://www.youtube.com/watch?v=gpFb635N2wo
- http://www.youtube.com/watch?v=PN5YiY0Wm6o
- How to identify colourless substances?
- Apply a locating agent on the chromatogram
- agents react with the substances to produce a coloured substance
- agents react with the substances to produce a coloured substance
- Apply a locating agent on the chromatogram
- applications of chromatography
- separate and identify dyes and drugs
- identify chemicals sprayed on vegetables
- determine purity of a sample
- determine purity of a sample
- identify chemicals sprayed on vegetables
- separate and identify dyes and drugs
- Advantages of chromatography
- allow analysis if a tiny amount of chemicals
- sensitive and highly reproducible
- allow analysis of many samples in a time.
- allow analysis of many samples in a time.
- sensitive and highly reproducible
- allow analysis if a tiny amount of chemicals
- How do we interpret the result of chromatography?
- If the chromatogram shows food colouring is a
mixture of 2 dyes, It is not pure.
- But is there is only one spot, it means it is pure,
- But is there is only one spot, it means it is pure,
- If the chromatogram shows food colouring is a
mixture of 2 dyes, It is not pure.
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