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6571949
Experimental Methods: Bio110-2016
Descripción
Workflow of experimental methods discussed in intro Bio
Sin etiquetas
western blots
pulse and chase
antibodies
gel electrophoresis
methods
biology
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Resumen del Recurso
Experimental Methods: Bio110-2016
Nucleotides
Do I want to know where they are made?
Yes
Pulse experiment... I know what radioactive precursor to use, right?
Autoradiography
Do I want to visualize the cell?
Microscopy... I know which kind I should be using, right? And how to prepare my cells or tissues?
Light or Flourescent Microscopy
Electron Microscopy
Do I want to differentiate between locations in the cell
Cell fractionization... I know which cell components separate at different speeds, right?
No, I want to know where they're going?
Pulse + Chase experiment
I just want to label them!
Proteins
Am I interested in a specific protein?
No, I'm interested in them all
Yes!
Well then I need an Antibody... I know how to make one, right?
Do I want to visualize where the protein localizes in the cell?
Fluorescent labeled antibody?
Gold labeled antibody?
Do I want to test which tissues, or individuals, or samples contain my protein?
Studying Tissues: Harvest Sample
Studying cell components: fractionization
Purify proteins. Use SDS
Gel Electrophoresis... before I visualize my protein of interest, I have to size separate all of them
Western Blot
Studying individuals: sample individuals
Recursos multimedia adjuntos
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