Cerrar
What must cells control in order to best respond to their environment?
Where is the ribosome binding site relative to the start of transcription on a bacterial gene?
RNAP
What makes for a strong promoter?
What is typically needed for a weak promoter to turn on?
Accessory transcription factors
repressors
corepressors
Arginine corepressor
Inducers
Lactose inducer
Activator
Maltose activator
How to determine difference between inducer vs activator (since phenotypic results are the same)
the basics of how to tell an inducer apart from a coactivator
Types of repressor-corepressor mutations
types of repressor-inducer mutations
types of activator-coactivator mutations
What is the point of specific regulatory mechanisms?
Global regulatory mechanisms
catabolite repression
Diauxic growth curve
Catabolite Activator Protein (CAP)
cAMP
Specific vs global regulatory mechanisms
regulation of the lac system
PTS system
What happens in the cell when glucose is PRESENT?
What happens in the cell when glucose is ABSENT?
How do cells recover from heat shock?
σ32 in normal conditions
σ32 in heat shock conditions
How do cells finally recover from heat shock?
SOS response to DNA damage
LexA
How do cells reset after SOS repair?
Stringent response
ppGpp
Functions of ppGpp
two-component signal transduction
