Vectors used to clone genes were derived originally from
proteins
plasmids
viruses
all of the above
b and c only
Restriction enzymes used in most cloning experiments
cut CNA into pieces for gene cloning
are naturally produced by bacteria cells to prevent viral infection
produce sticky ends on DNA fragments
All of the above are correct
only a and c are correct
DNA ligase is needed in a cloning experiment
to promote hydrogen bonding between sticky ends
to catalyze the covalent attachment of the backbone of DNA strands
to digest the chromosomal DNA into small pieces
Only a and b are correct
a, b, and c are correct
Let's suppose you followed protocols described in figures 20.2 and 20.3. Which experiment(s) would you conduct to confirm that a white colony really contained a recombinant vector with an inserted fragment of DNA?
Pick a bacterial colony and restreak on plates containing X-Gal to confirm that the cells really form white colonies.
Pick a bacterial colony, isolate plasmid DNA, digest the plasmid DNA with a restriction enzyme, and then preform gel electrophoresis with the DNA.
Pick a bacterial colony and test it to see it X-Gal is functional within the bacterial cells.
Pick a bacterial colony and retest it on ampicillin-containing plates to double-check that the cells are really ampicillin resistant.
Both c and d should be conducted.
Why is Taq polymerase used in PCR rather than other DNA polymerases?
Taq polymerase is a synthetic enzyme that produces DNA strands at a faster rate than natural polymerases.
Taq polymerase is a heat-stable form of DNA polymerase that can function after exposure to the high temperatures necessary for PCR.
Taq polymerase is easier to isolate than other DNA polymerases.
Taq polymerase is the DNA polymerase commonly produced by most eukaryotic cells.
All of the above are correct.
Let's suppose you want to clone a gene that has never been analyzed before by DNA sequencing. Which of the following statements do you agree with the most?
Do PCR to clone the gene because it is much faster.
Do PCR to clone the gene because it is very specific and gives a high yield.
You can't do PCR because you can't make forward and reverse primers.
Do cloning by insertion into a vector because it will give you a higher yield.
Do cloning by insertion into a vector because it is easier than PCR.
The method of determining the base sequence of DNA is termed
PCR
gene cloning
DNA fingerprinting
DNA sequencing
gene mapping
With regard to dideoxy sequencing, which of the following statements is false?
The dideoxy nucleotides are fluorescently labeled.
The dideoxy nucleotides cannot be incorporated into a growing DNA strand.
When incorporated into a DNA strand, the dideoxynucleotides prevent further growth of the strand.
The dideoxy sequencing method is used to determine the base sequence of DNA.
The dideoxy sequencing method requires the use of primers.
Organisms that carry genes that were introduced using molecular techniques are called
transgenics
recombinant DNA
mutants
genetically modified organisms
both a and d
DNA fingerprinting is used
to precisely identify an organism, such as the identification of specific strains of bacteria
as a forensics tool to provide evidence in a criminal case
to determine the genetic relationship between individuals
to determine the identity of an individual
for all of the above.
