DNA IV - Replication

Where does DNA replication begin in bacterial DNA? (3)

Draw a diagram of Bacterial DNA replication.

What class of enzymes are responsible for DNA synthesis?

How many DNA Pol' does E. Coli have? Which are of most importance in replication? (3)

What does a DNA Pol' need in order to synthesise DNA?

What is a primer?

What is the function of a primer?

Draw a diagram of the addition of a dNTP to the template strand of DNA

Draw a diagram of the DNA Polymerase reaction

What is the function of DNA Pol' i?

Draw a diagram showing exonuclease degradation of DNA

What conditions are necessary for exonuclease degradation by DNA Pol' i? (2)

What is "nick translation?"

Draw a diagram showing nick translation (4)

What is the function of 3'-5' exonuclease activity?

Draw a diagram showing 3'-5' exonuclease activity

Compare DNA Pol' i/iii (8)

Why does DNA Pol' iii have a much higher processivity?

What allows DNA Pol' iii to be so processive?

Draw a diagram showing the appearance of a Replication fork

What direction does DNA synthesis occur in?

Describe Stage 1 of DNA replication (5)

Describe Stage 2 of DNA replication (5)

Describe Stage 3 of DNA replication (8)

Describe Stage 4 of DNA replication (3)

Describe Stage 5 of DNA replication (3)

What is AZT?

How do DNA polymerase inhibitors work?(5)