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Histotechnology Test sobre Histotechnology, creado por Victoria Station el 04/12/2018.

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Histotechnology

Pregunta 1 de 90

1

What would not be seen in autolytic tissue?

Selecciona una de las siguientes respuestas posibles:

  • Nuclear detail lost

  • Stainability lost

  • Stain precipitation

  • Cells coming away from basement membrane

Explicación

Pregunta 2 de 90

1

What would be a "normal" specimen received in the grossing room?

Selecciona una de las siguientes respuestas posibles:

  • Tumor

  • Placenta

  • Surgical

  • Biopsy

Explicación

Pregunta 3 de 90

1

Cassettes:

Selecciona una de las siguientes respuestas posibles:

  • Are 3 cm deep

  • Must be labeled

  • Colours indicate day of week the tissue was processed

  • Has holes to allow the tissue to flow in an out

Explicación

Pregunta 4 de 90

1

Fixation:

Selecciona una de las siguientes respuestas posibles:

  • Functions by creating hydrogen bridges to prevent degredation

  • Typically shrinks, swells, and distorts tissue

  • Keeps bacteria and fungi viable

  • Allows the tissue to be stainable

Explicación

Pregunta 5 de 90

1

Which is not a factor affecting fixation?

Selecciona una de las siguientes respuestas posibles:

  • Time

  • Density of tissue

  • Temperature

  • Type of container

Explicación

Pregunta 6 de 90

1

Which is not a coagulant fixative?

Selecciona una de las siguientes respuestas posibles:

  • Zinc

  • Picric Acid

  • Acetic Acid

  • Alcohol

Explicación

Pregunta 7 de 90

1

Which non-coagulant fixative is also non-additive

Selecciona una de las siguientes respuestas posibles:

  • Osmium Tetroxide

  • Acetic Acid

  • Formalin

  • Gluteraldehyde

Explicación

Pregunta 8 de 90

1

How much concentrated formalin is needed to make 5.5L of 10% formalin?

Selecciona una de las siguientes respuestas posibles:

  • 55 ml

  • 55 L

  • 5500 ml

  • 550 ml

Explicación

Pregunta 9 de 90

1

To make 10% formalin, how many ml should be added to 230 ml of 100% formalin?

Selecciona una de las siguientes respuestas posibles:

  • 2300 ml

  • 0.23 L

  • 2070 ml

  • 2600 ml

Explicación

Pregunta 10 de 90

1

Which is true about formaldehyde?

Selecciona una de las siguientes respuestas posibles:

  • It is a coagulant fixative

  • It is composed of liquid formaldehyde in water

  • Add methanol to stop the formation of paraformaldehyde

  • Penetrates tissue slowly but fixes quickly

Explicación

Pregunta 11 de 90

1

The best fixative for DNA/RNA is?

Selecciona una de las siguientes respuestas posibles:

  • Carnoy

  • Picric Acid

  • Formalin

  • Gluteraldehyde

Explicación

Pregunta 12 de 90

1

Which is false about gluteraldehyde?

Selecciona una de las siguientes respuestas posibles:

  • Is a non-coagulant, additive fixative

  • Can be used to disinfect the cryostat

  • Can cause false positive reactions in period acid schiff

  • Creates methylene bridges in tissue to preserve it

Explicación

Pregunta 13 de 90

1

Mercuric Chloride

Selecciona una de las siguientes respuestas posibles:

  • Is a component in B5 fixative

  • Is a common fixative used in the histology lab

  • Mercuric pigment can be removed using alcoholic picric acid

  • Is a great fixative when tissue needs to be X-rayed

Explicación

Pregunta 14 de 90

1

What is false about osmium tetroxide?

Selecciona una de las siguientes respuestas posibles:

  • Fixes lipids

  • Used mostly for electron microscopy

  • Commonly a secondary fixative

  • Is a non-additive fixative

Explicación

Pregunta 15 de 90

1

Bouin's fixative:

Selecciona una de las siguientes respuestas posibles:

  • Is composed of picric acid, formaldehyde and mercuric chloride

  • Is the best preservative for glycogen

  • Does not stain tissue

  • Is commonly used as a secondary fixative

Explicación

Pregunta 16 de 90

1

Which is known to be a good general fixative?

Selecciona una de las siguientes respuestas posibles:

  • Gluteraldehyde

  • PAF / Zamboni

  • Picric Acid

  • Mercuric Chloride

Explicación

Pregunta 17 de 90

1

Select the correct statement regarding non-aqueous fixatives?

Selecciona una de las siguientes respuestas posibles:

  • Acetone should not be used for IHC since it destroys enzymes

  • Acetone is a good fixative since it does not shrink or harden tissue

  • Ethanol should not be used to fix frozen sections since it inhibits freezing

  • Ethanol should not be used to preserve glycogen as it is known to dissolve it

Explicación

Pregunta 18 de 90

1

Which fixative would be a good choice when RBCs need to be preseved

Selecciona una de las siguientes respuestas posibles:

  • Carnoy's

  • Picric Acid

  • Bouin's

  • Acetic Acid

Explicación

Pregunta 19 de 90

1

Oh no! Your tissue is autolytic! What should be done to prevent this?

Selecciona una de las siguientes respuestas posibles:

  • Tissue should be placed in fixative 10X greater than the tissue itself

  • Tissue should have been placed in fixative right after removal from the body

  • Tissue in fixative should be placed at 4 degrees Celcius

  • Tissue should have been frozen before fixation

Explicación

Pregunta 20 de 90

1

What would be proper fixation procedure for a large tissue?

Selecciona una de las siguientes respuestas posibles:

  • Large tissue should not be fixed

  • Large tissue must be cut into smaller pieces

  • Holes must be poked in the specimen to allow fixative to penetrate

  • Fixative must be injected into the specimen

Explicación

Pregunta 21 de 90

1

Which is true regarding the VIP Tissue Processor?

Selecciona una de las siguientes respuestas posibles:

  • Uses vacuum technology to infiltrate tissues

  • Hematoxylin is added to tint the tissue pink for easier embedding orientation

  • This processor is safer since it does not use heat

  • This is an open system

Explicación

Pregunta 22 de 90

1

Which is TRUE about dehydration?

Selecciona una de las siguientes respuestas posibles:

  • The dehydration process begins with the highest alcohol concentration then descends

  • Ethyl alcohol is the best for dehydration

  • Butanol is a good substitution because it is quick

  • Alcohol dehydrates tissue by removing xylene

Explicación

Pregunta 23 de 90

1

How would you be able to tell if a tissue was improperly dehydrated?

Selecciona una de las siguientes respuestas posibles:

  • Tissue would smell like xylene

  • Tissue would be soft

  • Impossible to tell until staining

  • Tissue would be dessicated

Explicación

Pregunta 24 de 90

1

What is the proper way to fix improper dehydration?

Selecciona una de las siguientes respuestas posibles:

  • First xylene, then several changes of alcohol, then xylene, then wax

  • Impossible to fix, request new specimen

  • First several changes of alcohol, then xylene, then wax

  • Several changes of wax

Explicación

Pregunta 25 de 90

1

After processing; tissue is soft, cloudy, and smells like alcohol. Why?

Selecciona una de las siguientes respuestas posibles:

  • Improper fixation

  • Improper dehydration

  • Improper clearing

  • Improper infiltration

Explicación

Pregunta 26 de 90

1

Which is not a clearing agent?

Selecciona una de las siguientes respuestas posibles:

  • Xylene

  • Toluene

  • Benzene

  • Chloridine

Explicación

Pregunta 27 de 90

1

Which is true considered a universal solvent?

Selecciona una de las siguientes respuestas posibles:

  • An example is Limonene

  • A solution that dehydrates and clears

  • An example is Xylene

  • A solution that dehydrates and infiltrates

Explicación

Pregunta 28 de 90

1

How would you fix improper infiltration?

Selecciona una de las siguientes respuestas posibles:

  • Increase the temperature of the wax

  • Remove wax with xylene, dehydrate with alcohol, then clear with xylene, then several changes of wax

  • Re-fix the tissue

  • Several changes of wax

Explicación

Pregunta 29 de 90

1

Which statement is TRUE?

Selecciona una de las siguientes respuestas posibles:

  • Soft wax allows for easy ribboning

  • Soft wax provides the most support

  • Hard wax melts at 45 degrees Celsius

  • Hard wax should not be used if thinner sections are required

Explicación

Pregunta 30 de 90

1

What is true about paraffin wax?

Selecciona una de las siguientes respuestas posibles:

  • Wax should be kept at least 10 degrees over the melting point

  • Typical melting point is 35-48 degrees

  • Overexposure to wax will cause tissue hardening and shrinking

  • Water-bath should be kept 2 degrees above the melting point

Explicación

Pregunta 31 de 90

1

What is an advantage to using a microwave for processing?

Selecciona una de las siguientes respuestas posibles:

  • Clearing step can be skipped

  • Infiltration step can be skipped

  • Dehydration step can be skipped

  • Microwaves would never be used for processing

Explicación

Pregunta 32 de 90

1

A technologist is viewing several embedded tissues. Which one would need to be re-embedded?

Selecciona una de las siguientes respuestas posibles:

  • Several small biopsies are placed together

  • Red dot is facing towards you when cutting

  • Two pieces of small bowel are orientated so that the lumens are facing eachother

  • Transverse cut of tubular tissue

Explicación

Pregunta 33 de 90

1

Which of these is proper troubleshooting?

Selecciona una de las siguientes respuestas posibles:

  • Incorrect orientation of tissue when embedding - discard the tissue and request new specimen

  • Poor processing of tissues - check solutions and machine

  • Tissue is over-dehydrated - increase time in alcohol

  • NBF precipitate is found in the tubing of the processor - perform a picric acid flush

Explicación

Pregunta 34 de 90

1

Troubleshoot this cutting situation: what is the most likely cause of crooked ribbons?

Selecciona una de las siguientes respuestas posibles:

  • Block not chilled enough

  • Cutting too slowly

  • Dull blade

  • Block and knife not parallel to eachother

Explicación

Pregunta 35 de 90

1

Select the correct statement about decalcification

Selecciona una de las siguientes respuestas posibles:

  • Tissue must be > 3 mm in order to be decalcified properly

  • Calcium is soluble at pH 4.5

  • Acid methods are usually slow

  • Decalcification increases stainability of bone tissue

Explicación

Pregunta 36 de 90

1

What is the most accurate way to determine the end point of decalcification?

Selecciona una de las siguientes respuestas posibles:

  • Mechanical methods such as poking

  • Chemical methods such as ammonium hydroxide and ammonium oxalate which check for the precipitation of calcium oxalate

  • Cutting the bone tissue

  • Radiography

Explicación

Pregunta 37 de 90

1

When looking at a H&E stained bone under the microscope, the technologist notices that there is many dark blue/purple staining areas. What could this mean?

Selecciona una de las siguientes respuestas posibles:

  • Bone was stained too long in hematoxylin

  • Bone was underdecalcified

  • Bone was improperly fixed

  • Bone was not stained with the correct stain

Explicación

Pregunta 38 de 90

1

What is the incorrect procedure when using the cryostat?

Selecciona una de las siguientes respuestas posibles:

  • Disinfect daily with sodium hypochlorite

  • Use cryostat when rapid diagnosis is required or when fat or enzymes need to be demonstrated.

  • If a tissue with known TB requires cutting, disinfect afterwards with formaldehyde vapor

  • If tissue is sticking the antiroll plate may be too warm

Explicación

Pregunta 39 de 90

1

Which is the correct use for each microscope?

Selecciona una de las siguientes respuestas posibles:

  • Fluorescent microscope for Acid Fast Bacilli

  • Polarizing microscope for Oil Red O

  • Compound light microscope for Acridine Orange

  • Electron microscope for H&E

Explicación

Pregunta 40 de 90

1

Select the correct statement about microtomes:

Selecciona una de las siguientes respuestas posibles:

  • "Roughing in" is usually done at 20 micrometers

  • The clearance angle is usually set at 10 degrees

  • Speed of cutting has no effect on ribbon quality

  • In a rotary microtome, the knife moves side to side

Explicación

Pregunta 41 de 90

1

Select the correct statement about automatic stainers:

Selecciona una de las siguientes respuestas posibles:

  • With linear stainers, time in solution can be varied by adding more containers

  • With robotic stainers, the programs cannot be changed

  • Robotic stainers are good for progressive staining

  • Linear stainers are good for regressing staining

Explicación

Pregunta 42 de 90

1

What is proper water bath procedure?

Selecciona una de las siguientes respuestas posibles:

  • Water bath should be kept at 5 - 10 degrees above the melting point of wax

  • Tap water should always be used to fill water baths

  • Parched earth artifact can result if the water bath is too cold

  • Water bath should be cleaned after each ribbon

Explicación

Pregunta 43 de 90

1

A technologist is cutting many tissues for different areas of the lab. Which of the following is an incorrect procedure?

Selecciona una de las siguientes respuestas posibles:

  • Slides for IHC were placed on a hotplate for drying.

  • Slides with biospy were cut using special techniques with 6 tissues per slide

  • Slides for neurology were placed in a 37 degree oven for drying

  • Slides for routine H&E were placed in a 60 degree oven for drying

Explicación

Pregunta 44 de 90

1

A student is concerned about contracting TB from patient samples. What process would be of the least concern to the student?

Selecciona una de las siguientes respuestas posibles:

  • Embedding

  • Grossing

  • Autopsy

  • Frozen sectioning

Explicación

Pregunta 45 de 90

1

Which is considered to be a correct safe procedure?

Selecciona una de las siguientes respuestas posibles:

  • Placing used blades in the garbage

  • Fixing tissue that is known to be TB positive with formalin

  • Storing glacial acetic acid on the counter top

  • Fixing CJD positive tissue with formalin

Explicación

Pregunta 46 de 90

1

Which is not an element of a dye molecule?

Selecciona una de las siguientes respuestas posibles:

  • metachrome

  • benzene ring

  • auxochrome

  • chromaphore

Explicación

Pregunta 47 de 90

1

What is a correct statement about staining:

Selecciona una de las siguientes respuestas posibles:

  • A method of nuclear staining is acidic dye

  • A method of nuclear staining is dye + metal mordant

  • Cytoplasmic staining occurs from the interaction of dye with neutral molecules

  • Lipids are stained because the dye has a greater affinity to the solution than the fat

Explicación

Pregunta 48 de 90

1

Which is considered a basic auxochrome?

Selecciona una de las siguientes respuestas posibles:

  • -NH2

  • -COOH

  • -OH

  • -SO4

Explicación

Pregunta 49 de 90

1

Which statement is correct?

Selecciona una de las siguientes respuestas posibles:

  • Basic dyes will have a negative auxochrome

  • Basic dyes will produce chloride salt

  • Acid dyes are cationic

  • Acid dyes produce magnesium salt

Explicación

Pregunta 50 de 90

1

Which is a true statement about dyes?

Selecciona una de las siguientes respuestas posibles:

  • Modifiers will affect staining time

  • Sulphonics makes acidic dyes basic

  • Dye binding can be affected by pH

  • Orthochromic dye will stain a different colour than the dye colour

Explicación

Pregunta 51 de 90

1

Which is not a metachromatic component?

Selecciona una de las siguientes respuestas posibles:

  • AFB

  • Mucin

  • Mast Cell

  • Amyloid

Explicación

Pregunta 52 de 90

1

Why are thymol crystals added to dye? ;)

Selecciona una de las siguientes respuestas posibles:

  • Prevent mold growth

  • Enhance staining

  • Makes the dye more acidic

  • Makes the dye fluorescent

Explicación

Pregunta 53 de 90

1

Which is not a natural dye?

Selecciona una de las siguientes respuestas posibles:

  • Hematoxylin

  • Orcein

  • Acridine Orange

  • Saffron

Explicación

Pregunta 54 de 90

1

Which pairing for decolorization is incorrect?

Selecciona una de las siguientes respuestas posibles:

  • Weak acid differentiating basic dyes ex. PTA/PMA for Biebrich Scarlet

  • Weak base differentiating acid dyes ex. PTA/PMA for Biebrich Scarlet

  • Excess mordant differentiating ex. iron alum for Verhoeff

  • Alcoholic differentiation ex. alcohol for eosin

Explicación

Pregunta 55 de 90

1

A technologist needs to prepare a hematoxylin for use in Masson's Trichrome. Which would be the correct procedure?

Selecciona una de las siguientes respuestas posibles:

  • Oxidize the hematoxylin with sodium iodate

  • Prepare hematoxylin using aluminum since it can withstand strong acids in the stain

  • After preparing the hematoxylin, pour directly into the coplin jar for staining

  • Gill's hematoxylin should be prepared for this stain

Explicación

Pregunta 56 de 90

1

A technologist has prepared several different hematoxylins for use. Which one of them is paired correctly for use?

Selecciona una de las siguientes respuestas posibles:

  • Gill's hematoxylin for use in Alcian Blue

  • Mayer's hematoxylin for Oil Red O

  • Harris hematoxylin for Masson's Trichrome

  • Weigert's hematoxylin for routine H&E

Explicación

Pregunta 57 de 90

1

What is true about Hematoxylin and Eosin?

Selecciona una de las siguientes respuestas posibles:

  • Hematoxylin stains indirectly

  • Is never a regressive stain

  • Cannot be used to stain bone tissue

  • The best substitute for eosin would be phenol red

Explicación

Pregunta 58 de 90

1

A technologist is reviewing a manual H&E control slide when they notice that the nuclei have not stained. Which of the following options is likely NOT be a reason for this problem.

Selecciona una de las siguientes respuestas posibles:

  • Tissue was left in acid alcohol for 20 minutes during coffee break

  • Aluminum / iron was not present in the hematoxylin

  • The hematoxylin was not filtered properly

  • The hematoxylin was stored in a clear glass jar

Explicación

Pregunta 59 de 90

1

A technologist reviewing an H&E control has noticed that the cytoplasm is staining very dark. Which is not a likely cause?

Selecciona una de las siguientes respuestas posibles:

  • Tissue was cut at 3 micrometers

  • Eosin is too concentrated

  • Eosin was not left in alcohol long enough

  • Tissue was left in eosin for too long

Explicación

Pregunta 60 de 90

1

A technologist is preparing to perform Masson's trichrome on a kidney slide. Which of the following procedures is INCORRECT?

Selecciona una de las siguientes respuestas posibles:

  • Places slide in Bouin's for 1 hour at 56 degrees.

  • Counterstain in Light Green

  • Places slides in Biebrich Scarlet Acid Fuchsin before PTA/PMA

  • Uses Mayer's Hematoxylin to stain nuclei

Explicación

Pregunta 61 de 90

1

A technologist is reviewing the Masson's Trichrome control. Which of the following stained components is cause for concern?

Selecciona una de las siguientes respuestas posibles:

  • Collagen has stained blue

  • Cytoplasm has stained red

  • RBCs have stained red

  • Nucleic acids have stained red

Explicación

Pregunta 62 de 90

1

A technologist is preparing to perform Gordon and Sweets on a liver tissue. Which of the following is the CORRECT procedure?

Selecciona una de las siguientes respuestas posibles:

  • Ensure than silver solution is neutralized with HCl before disposal

  • Place the slides in a thin walled coplin jar to ensure good heating in the microwave

  • Bleach tissue in Oxalic Acid after Potassium Permanganate

  • Counterstain in Light Green

Explicación

Pregunta 63 de 90

1

Which staining method is NOT correctly paired with the type of metallic impregnation?

Selecciona una de las siguientes respuestas posibles:

  • Gomori Burnter - argentaffin

  • Grocott - argyrophilic

  • von Kossa - substitution

  • Gordon and Sweets - argyrophilic

Explicación

Pregunta 64 de 90

1

A technologist is reviewing the control for Verhoeff's van Gieson. Which of the following errors is paired with the correct solution?

Selecciona una de las siguientes respuestas posibles:

  • Elastic fibers cannot easily be seen on the brain control slide so it was placed in Verhoffs stain for a longer period of time

  • Nuclei are not staining so a new solution of Verhoffs made and used

  • The stain looks very muddy so it was placed back into gold chloride for a longer time

  • Elastic fibers are too pale so the slide was placed back into van Gieson stain for longer

Explicación

Pregunta 65 de 90

1

Which is the correct statement about elastic stains?

Selecciona una de las siguientes respuestas posibles:

  • Orcein is specific for elastic fibres

  • Orcein will stain elastic fibres brown

  • Aldehyde fuchsin will stain elastic fibres green

  • Aldehyde fuchsin is a regressive method

Explicación

Pregunta 66 de 90

1

A technologist is preparing to perform Toludine Blue. What should the technologist NOT do?

Selecciona una de las siguientes respuestas posibles:

  • Take the slides down to water before staining

  • Place the slides in Toludine Blue to stain for mast cells

  • Place the slides in Light Green as a counterstain

  • Differentiate with alcohol then clear and mount

Explicación

Pregunta 67 de 90

1

A technologist is confused why their Alcian Blue control slide did not demonstrate mucins. Which would NOT be a cause for this to happen?

Selecciona una de las siguientes respuestas posibles:

  • Stomach tissue was used as the control

  • The tissue was placed in 3% acetic acid before Alcian Blue dye

  • The tissue was placed in Acetic Acid following Alcian Blue for 10 minutes

  • The pH of Alcian Blue was 6.5

Explicación

Pregunta 68 de 90

1

Oh no! Our Periodic Acid Schiff's stain didn't work (again).Which of the following would NOT be a possible cause?

Selecciona una de las siguientes respuestas posibles:

  • Schiff's reagent was tested before use using formalin and the reaction became a deep blue colour

  • Grocott's method was performed earlier so the periodic acid solution was saved and used agian

  • Metabisulfite rinse was prepared using 100% HCl straight from the bottle

  • Tissues were washed for 10 minutes in water after Schiff's

Explicación

Pregunta 69 de 90

1

Which statement is true regarding Periodic Acid Schiffs?

Selecciona una de las siguientes respuestas posibles:

  • Schiff's reagent is composed of Acid Fuchsin and Sulpheric Acid

  • Periodic acid is the secondary oxidizer

  • Sodium Metabisulfite rise is what brings the pink colour to the tissue

  • Fungi will stain pink

Explicación

Pregunta 70 de 90

1

Which is FALSE about Mayer's Mucicarmine?

Selecciona una de las siguientes respuestas posibles:

  • Metanil Yellow is the primary stain

  • Mucicarmine stains acid mucins

  • Gill's hematoxylin should not be used as the nuclear stain

  • Cryptococcus can also be demonstrated with this method

Explicación

Pregunta 71 de 90

1

A technologist is preparing to stain for amyloid. Which procedure is correct?

Selecciona una de las siguientes respuestas posibles:

  • The technologist double checked to ensure sections were cut at 4um

  • The doctor would like to differentiate between primary and secondary amyloidosis so the slide was pretreated with potassium permangonate

  • Slides were placed in neutral red to stain for amyloid

  • The technologist did not know how to use the polarizing microscope so birefringence was not checked on the control

Explicación

Pregunta 72 de 90

1

The pathologist has requested an Oil Red O stain for a frozen tissue. Which of the following procedures is INCORRECT?

Selecciona una de las siguientes respuestas posibles:

  • The tissue was previously fixed in formalin

  • A frozen section was cut for staining

  • Tissue was taken down to water before staining

  • The tissue was placed in hematoxylin after Oil Red O

Explicación

Pregunta 73 de 90

1

Which is FALSE about Luxol Fast Blue stain?

Selecciona una de las siguientes respuestas posibles:

  • Luxol Fast Blue dye is differentiated with an acid

  • The control tissue is cerebellum

  • Eosin is the counterstain

  • Luxol Fast Blue stains myelin

Explicación

Pregunta 74 de 90

1

A technologist notices an endogenous pigment on their slides. What is it?

Selecciona una de las siguientes respuestas posibles:

  • mercury

  • tattoo

  • carbon

  • melanin

Explicación

Pregunta 75 de 90

1

A technologist is performing Von Kossa. Which procedure is INCORRECT?

Selecciona una de las siguientes respuestas posibles:

  • Tissue is placed in silver nitrate and then microwaved for the appropriate time

  • Placenta is used as the calcium control

  • The tissues are placed in nuclear fast red or neutral red

  • The technologist decides the control passed since the calcium stained brown

Explicación

Pregunta 76 de 90

1

A technologist is performing Perl's Prussian Blue on a control and a patient tissue. The technologist checks the PATIENT'S slide to find that no iron has been stained! What is the most likely cause for this result?

Selecciona una de las siguientes respuestas posibles:

  • Slide was placed in eosin

  • Patient tissue does not contain iron

  • Slide was taken down to distilled water

  • Slide was placed in HCl + Potassium Ferrocyanide

Explicación

Pregunta 77 de 90

1

Which is a true statement regarding Gomori Burtner stain?

Selecciona una de las siguientes respuestas posibles:

  • This stain is used to demonstrate argentaffin granules

  • Gold chloride is a fixing agent

  • Hematoxylin is the counterstain

  • Melanin cannot oxidize silver therefore a reducer such as Sodium Thiosulfate is required

Explicación

Pregunta 78 de 90

1

A technologist needs to stain for Blastomyces, which stain should they select?

Selecciona una de las siguientes respuestas posibles:

  • Ziehl Neelson

  • Gram

  • Geimsa

  • Grocott

Explicación

Pregunta 79 de 90

1

A sample for AFB is arriving in the lab for staining. What should be done?

Selecciona una de las siguientes respuestas posibles:

  • After fixation, Gram stain the tissue to detect the bacteria

  • Wear N-95 for frozen section

  • Place the specimen in Carnoy's for fixation then stain with Ziehl Neelson

  • After fixation, take down slides to tap water before staining

Explicación

Pregunta 80 de 90

1

Why do AFB stain red with Ziehl Neelson?

Selecciona una de las siguientes respuestas posibles:

  • Not enough Acid Alcohol was used to decolourize the AFB

  • Methylene blue stain was missed

  • Nature of their cell wall

  • Because they are Gram negative

Explicación

Pregunta 81 de 90

1

Which component of Gram stain is correctly matched with its purpose?

Selecciona una de las siguientes respuestas posibles:

  • Crystal violet - stains Gram negative bacteria

  • Acetone / Alcohol - decololourizes Gram positive bacteria

  • Basic Fuchsin - stains acid fast bacteria pink

  • Picric Acid - stains bacteria yellow

Explicación

Pregunta 82 de 90

1

A technologist is having trouble with Grocott stain. Which of the following problems is correctly solved?

Selecciona una de las siguientes respuestas posibles:

  • Fungi are stained black - the slide is placed back into gold chloride for more toning until fungi are brown

  • Fungi, nuclei, and other tissues are staining black - the slide is good

  • No fungi have been stained - the wrong concentration of periodic acid was used

  • Fungi are brown - the slide is good

Explicación

Pregunta 83 de 90

1

Which of these uncommon stains is correctly paired?

Selecciona una de las siguientes respuestas posibles:

  • MSB stain for bile

  • Warthin-Starry for H. pylori

  • Steiner for spirochetes

  • Hall for fibrin

Explicación

Pregunta 84 de 90

1

Which of the following is NOT a correct procedure in IHC?

Selecciona una de las siguientes respuestas posibles:

  • Deparaffinize and hydrate to water

  • apply antibody

  • stain nuclei with hematoxylin

  • counterstain with hematoxylin

Explicación

Pregunta 85 de 90

1

Which is true about antigen sites in tissues for IHC?

Selecciona una de las siguientes respuestas posibles:

  • Tissue must be washed with water to remove crosslinking from formalin

  • Proteolytic enzymes will destroy the antigen sites

  • Heat methods help cleave the bonds

  • Enzymatic methods will unfold the bonds

Explicación

Pregunta 86 de 90

1

Which of these solutions is NOT properly disposed of?

Selecciona una de las siguientes respuestas posibles:

  • Picric acid in a container

  • Alcohol in a container

  • Sodium Thiosulfate down the sink

  • Silver Nitrate down the sink

Explicación

Pregunta 87 de 90

1

Which pairing of control with stain will not work?

Selecciona una de las siguientes respuestas posibles:

  • Kidney for Masson's Trichrome

  • Placenta for von Kossa

  • Cerebrum for Luxol Fast Blue

  • Stomach for Alcian Blue

Explicación

Pregunta 88 de 90

1

Why should gloves be worn when cutting tissues on the microtome?

Selecciona una de las siguientes respuestas posibles:

  • To prevent the blade from getting scratched

  • To prevent your hands from getting infected

  • To prevent skin cells from getting on the microscope slides

  • To prevent yourself from getting cut

Explicación

Pregunta 89 de 90

1

What would not be a likely reason for seeing pale staining elastic fibres for Verhoeff van Gieson?

Selecciona una de las siguientes respuestas posibles:

  • Left in Sodium Thiosulfate for too long

  • Left in Ferric Chloride too long

  • Left in Van Gieson stain too long

  • Left in Verhoeff stain too long

Explicación

Pregunta 90 de 90

1

Which would not be a reason for seeing pale cytoplasmic staining on H&E stained tissue?

Selecciona una de las siguientes respuestas posibles:

  • pH of eosin is 8.0

  • Sections were cut at 8 micrometers

  • Tissue left in alcohol for too long

  • Tissues were rinsed in water after blueing

Explicación

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