WSU BIO 2200 Lab Practical

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Microbiology Lab is Biosafety Level 2

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Bacteria are ( ubiquitous, formite, thick ), since they can be found all over and in every environment.

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Bacteria have a ( negative charge, positive charge ). Because of this basic dyes, with their ( positive charge, negative charge ), stain the ( bacteria, background ). Acidic dyes, with their ( negative charge, positive charge ), stain the ( background, bacteria ).

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Examples of acidic dyes:

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Which microscope was used in the lab?

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Limit of Resolution:
D = ( Wavelength, NA condensor, NA objective lens ) / (( Wavelength, NA condensor, NA objective lens ) + ( Wavelength, NA condensor, NA objective lens ))

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Smears should:

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Purpose of heat-fixing

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Pure cultures should have many different kinds of bacteria

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Klebsiella Pneumoniae has a capsule

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Choose what best describes capsules

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Gram-positive bacteria

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Order of Gram staining:
1. ❌ with ❌
2. ❌ with ❌
3. ❌ with ❌
4. ❌ with ❌

Primary stain

Crystal Violet

Mordant

Gram's Iodine

Decolorizer

95% Ethanol

Counterstain

Safranin

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Which describes chemotaxis?

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Which describes lophotrichous flagella?

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Final lab practical quiz...good luck!

WSU BIO 2200 Lab Practical

Microbiology Lab is Biosafety Level 2

Bacteria are ubiquitous formite thick( ubiquitous, formite, thick ), since they can be found all over and in every environment.

Examples of acidic dyes:

Which microscope was used in the lab?

Limit of Resolution: D = Wavelength NA condensor NA objective lens( Wavelength, NA condensor, NA objective lens ) / (Wavelength NA condensor NA objective lens( Wavelength, NA condensor, NA objective lens ) + Wavelength NA condensor NA objective lens( Wavelength, NA condensor, NA objective lens ))

Smears should:

Purpose of heat-fixing

Pure cultures should have many different kinds of bacteria

Klebsiella Pneumoniae has a capsule

Choose what best describes capsules

Gram-positive bacteria

Order of Gram staining: 1. ❌ with ❌ 2. ❌ with ❌ 3. ❌ with ❌ 4. ❌ with ❌

Which describes chemotaxis?

Which describes lophotrichous flagella?

The motility test media is 0.4-0.5% agar and contains TTC (2,3,5-triphenyltetrazolium chloride)

Disadvantages of the motility test:

TTC is colorless when and red when .

Escherichia coli is motile and Staphylococcus epidermidis is non-motile.

Bacteria are ( ubiquitous, formite, thick ), since they can be found all over and in every environment.

Limit of Resolution:
D = ( Wavelength, NA condensor, NA objective lens ) / (( Wavelength, NA condensor, NA objective lens ) + ( Wavelength, NA condensor, NA objective lens ))

Order of Gram staining:
1. ❌ with ❌
2. ❌ with ❌
3. ❌ with ❌
4. ❌ with ❌