Anaerobic Metabolism - the TCA Cycle

Fill in the blanks below to give a general description of the TCA cycle.
1. (2C) combines with (4C) to form (6C).
2. is isomerised to (6C).
3. Citrate is decarboxylised to (5C)
4. is decarboxylised to (4C)
5. is converted to , then , then , then finally back to .
6. The cycle repeats.

Fill in the blanks below to describe the link reaction.
P reacts with c to form a bond. This forms an a molecule and a c. This forms + H+ from NAD. This reaction is catalysed by .

Fill in the blanks below to summarise each reaction of the TCA cycle based on the type of reaction it is.
1.
2.
3.
4.
5.
6.
7.
8.

Fill in the blanks below to describe the reactions of the TCA cycle.
1. First, acetyl CoA and combine to form the 6-carbon compound . This is catalysed by citrate synthase.
2. Then, is isomerised to . This is catalysed by .
3. is decarboxylated to . This is catalysed by and forms NADH and H+ from .
4. is decarboxylated to . This involves addition of and is catalysed by . This also forms .
5. is cleaved into s and coenzyme A by . This forms from and inorganic phosphate.
6. is decarboxylated into . This is catalysed by and forms .
7. is hydrated to form . This is catalysed by .
8. is decarboxylated into . This is catalysed by and forms .
9. The cycle repeats.

Fill in the blanks below to describe oxidative phosphorylation.
1. The proton pump on the mitochondrial membrane moves into the . This id driven by .
2. The of the intermembranal space .
3. A gradient of ions is produced.
4. Protons move back into the via causing it to .
5. energy is used to synthesise from ADP and Pi.

Fill in the blanks below to describe the yield from the TCA cycle.
Each TCA cycle yields NADH molecules. x = ATP from NADH.
Each TCA cycle yields FADH2 molecule. x = ATP from FADH2.
Each TCA cycle yields GTP molecule. x = ATP from GTP.
This totals to ATP per TCA cycle.

Fill in the blanks below to describe the feedback inhibition of the 3 irreversible enzyme steps of the TCA cycle.
Isocitrate dehydrogenase is inhibited by and activated by .
Ketoglutarate dehydrogenase is inhibited by and s.
Citrate synthase is inhibited by N and .

Fill in the blanks below to describe the links between the TCA cycle and biosynthesis.
Pyruvate can be converted to by .
can be used to form fatty acids and sterols.
can be involved in transamination reactions to form g.
can form pyruvate in a reaction catalysed by .
Oxaloacetate can be converted to by and then into .
Oxaloacetate can be involved in reactions to form a.