Which Gprotein causes the activation Phospholipase CBeta?
Gq
Gs
Gi
How is Phospholipase CBeta activated?
By GTP binding to the alpha subunit of the Gq protein
By ATP binding to the alpha subunit of the Gq protein
By GMP binding to the alpha subunit of the Gq protein
What is broken down by phospholipase CBeta?
Phosphatidynositol 4,5 bisphophate
Phosphatidynositol 3,5 bisphophate
Phosphatidynositol 2,4 bisphophate
Which product of the PCbeta pathway causes the release of calcium from the ER?
IP3
Diacylglycerol
What role(s) does the released Ca perform?
Activates protein kinase C
Activates protein kinase B
Activates various Ca dependent protein kinases
What residues are phosphorylated by the protein kinases activated by this pathway?
Serine
Threonine
Tyrosine
Which subunit of Protein Kinase C contains the pseudosubstrate domain?
PS
C1A
C4
What is the function of subunut C1A
DAG binding site
Calcium binding site
Phosphatidyl serine binding site
Do Ca 2+ and phosphatidyl serine bind to subunit C2?
Yes
No
Which subunits are part of the catalytic domain?
C3
C5
ATP binds to C2, True or False?
True
False
When IP3 causes the increase in cytosolic calcium, what happens to protein kinase C?
It moves to the membrane and binds with DAG
It remains in the cytoplasm and binds with DAG
Once protein kinase C has bound to DAG in the membrane, what does it then bind to to release the pseudosubstrate from its active site?
Phosphatidylserine
Phosphatidylglycine
Phosphatidylglutamate
Which of the following is a tumor promoting agent?
Phorbol ester
Orphol ester
Phenyl ester
How do tumor promoting agents effect Phospholipase CBeta?
They bind without being degraded, so it becomes hyperactive
They stop the alpha subunit binding, so it becomes unreactive
They cause the multiplication of the number of PCbeta enzymes in the cell, causing hyperactivity
