What is so special about cGMPPK?
It's anti-parallel
The subunits of the dimer can swap roles of damage occurs
It's only found in cardiac cells
How many cGMP bind per monomer?
2
4
6
1
3
Which order do the subunits go in (from N to C terminus) A= Dimerisation Domain B- Catalytic Domain C= Autoinhibitory Hinge Region D= cGMP binding sites 1 & 2
A-C-D-B
A-B-D-C
C-A-D-B
C-D-A-B
What is the purpose of the autoinhibitory hinge region?
Acts as a pseudosubstrate
Causes inhibition after a certain amount of activity
What motif does cGMP Stimulated Protein Kinase Phosphorylate?
Arg-Ser-X-Arg
Arg-Thr-X-Arg
Arg-Arg-X-Ser
Arg-Arg-X-Thr
What is the role of this protein?
The activation of cGMP dependent ion channels
Activates cGMP dependent phosphodiesterases
The activation of cGMP dependent proteins
Inhibits cGMP dependent phosphodiesterases
What is required for NO to be synthesized?
NO synthase
FMD/FAD
O2
NADP
NADPH
FMD
Tetrahydrobiopterin
Which of the following stimulate NO production? A- AcH B- Bradylunin C- Nicotine D- Thrombin E- Cl- F- Ca2+
A, B, D, F
A, D, E, F
B, C, D, F
Which system is NO part of?
Paracrine
Juxtracrine
Autocrine
Endocrine
NO is synthase a monomer- True or False?
True
False
How many types of NO synthase are there?
Constitutive
Inducible
Constitutive & Inudcible
Why is NO produced by iNOS (Immune NO Synthase)
It kills of pathogens
It's used to signal immune cells
It's used to bind and immobalise pathogens
NO Synthase produces a few mmol of NO over a minute- True or False?
False- its pmol over a minute
False, it's micromol in a few seconds
False, its pmol over a few seconds
NO Synthase contains the following, but in which order? A-NADPH binding site B-Calmodulin Binding Site C-FMN centre D- FAD centre E-Haeme group F- Arg, O2 and Tetrahydropterin (Active site)
E-F-B-C-D-A
A-D-B-C-F-E
E-F-A-C-D-B
Why are vascular endothelial cells equipped to cause vasodilation?
It allows them to reduce blood pressure according to need
It allows them to increase blood flow to wounded areas
It prevents damage to veins under high pressure
Which neurotransmitter effects which cells? A- Ach B-Vasopressin
Vascular Endothelial-A Skeletal Muscle-B
Vascular Endothelial- A&B Skeletal Muscle-A
Vascular Endothelial- A Skeletal Muscle-A&B
What is the role of nitroglycerin?
An explosive
It liberates NO from tissues
Causes an increase in NO synthesis
What is the role of viagra?
Inhibits phosphodiesterases
Prevents cGMP breakdown
Prolongs NO production
