What is the role of the Rb Susceptibility gene?
Nuclear transcription factor
Nuclear phosphatases
Nuclear phosphorylases
What protein does Rb bind to?
E2F
FF2
EF2
What is the role of the protein bound to Rb?
Transcription factor
Kinase
Phosphatase
What are the target genes of the Rb bound protein?
dNTP producing genes
Transcription factor producing genes
Growth factor producing genes
How is Rb phosphorylated and deactivated?
By Cyclin
By CDK
By a cyclin/CDK dimer
What protein phosphorylates CDK?
CAK
CAD
CAB
Which forms first during G1?
CDK/Cyclin D
CDK/Cyclin E
What causes the production of Cyclin/CDK E &D?
Jun Activity
AP1 Activity
Myc Activity
Which is required to activate E2F?
Cyclin/CDK D
Cyclin/CDK E
Both
Neither
Name 3 CDK inhibitors
p13
p21
p12
p16
p27
p19
Which of the following binds to CDK, & twists its head to block the active site?
Which of the following bind to cdk with cyclin and physically block the active site?
How are the CDK inhibitors controlled?
Phosphorylation & degredation
Phosphorylation & activation
How long is the bp sequence p53 binds to?
10 bp
12 bp
15 bp
Put the following p53 domains in order A- Signalling Domain B- Damaged DNA binding domain C-Oligomerization Domain D- Activation Domain E- DNA binding domain
D-A-E-C-B
D-A-C-E-B
A-D-C-E-B
B-A-D-E-C
How does p53 work in its active form?
As a monomer
As a dimer
As a tetramer
What does p53 bind to when active?
TATA boxes
E-boxes
Damaged DNA
What can cause the activation of p53?
Acetylation
Phosphorylation
Ionising radiation
What does p53 do if activated in G1?
Repairs DNA
Reverts the cell back to G0
Forces cell through mitotic cycle
What happens if p53 is activated after G1?
Bax is transcribed- it initiates apoptosis
Bax is transcribed- It initiates S phase
Bax is transcribed- It inhibits M phase
Where do mutations causing non-function tend to occur in p53?
The DNA binding domain
The damaged DNA binding domain
The signalling domain
How is p53 removed from the cell?
MDM2
MDM
MDM1
