Cyt C Oxidase & F1F0

1

What is the main role of cytochrome aa3?

1

Complex IV contains how many subunits?

1

Complex IV doesn't transfer protons across the inner mitochondrial membrane

1

Which ions does it contain, and how many of each?

1

Cytochrome aa3 complex consists almost entirely of alpha helices

1

Put the following in order
A- Cyt c
B- CuA centre
C- Cyt a
D- Cyt a3-CuB centre
E - Water
•Transfer from cyt a3-CuB centre to oxygen

1

How many protons are pumped by complex 4, and how many are used to make water (per Oxygen molecule)?

1

Which conformation of Complex IV has the low affinity for protons?

1

Protons bind to Complex IV during Conformation B

1

What happens during conformation B that lowers the Complex IV's affinity for protons?

1

No question, just look at it and learn it

1

ATP synthase is found on the inner mitochondrial membrane

1

ATP synthase is divided into two sections, F1 & F0
Which is peripheral and which is integral?

1

Name the function of ATP synthase

1

If ATP is high, and the proton gradient is low, what will Complex V do instead?

1

Protons flow through F1 to power the ATP synthesis in F0

1

Name the subunits of F1

1

Which of the following subunit(s) are the largest in F1?

1

What is the role of the delta subunit in F1?

1

The epsilon subunit of F0 connects the gamma to the C subunit

1

F0 contains how many subunits?

1

The F1 subunit is...

1

The Y subunit of F1 is made of 2 beta sheets that have been bent, but are bound at the base

1

When the gamma subunit is in contact with a beta subunit, the ATP catalysis site is in it's _ formation

1

A subunit contains ADP + Pi- what conformation is it in?

1

Whilst the subunit is replacing ATP with ADP + Pi, the gamma subunit remains in plance

1

What order do the three conformations work in?
Assume you start with the Loose formation

1

State the characteristics of the C subunit of F0

1

How many monomers make up the C subunit?

1

The C subunit contains 3 rings of monomer

1

Why is there a variance across species of the number of monomers in the C subunit?

1

More subunits require more protons to move them, so more subunits is less efficient

1

How is the A subunit described?

1

Protons passing through the A subunit...

1

Each subunit can hold 2 protons

1

The momevent of the protons around the C subunit powers what?

1

The rotation of the F1 has been proved by attaching a microtubule filament to the F1 head and examining it under the microscope

1

Only the gamma subunit of the F1 complex rotates

1

How is the Alpha/Beta spheroid of F1 held in place?

1

For every _degree rotation, 1 molecule of ATP is produced

1

The mechanism of the gamma subunit causes an affinity change for ATP/ADP + Pi, driving the cycle

(Membrane Proteins) Structural Basis for Biological Function Test sobre Cyt C Oxidase & F1F0, creado por gina_evans0312 el 18/12/2013.

Cyt C Oxidase & F1F0

What is the main role of cytochrome aa3?

Complex IV contains how many subunits?

Complex IV doesn't transfer protons across the inner mitochondrial membrane

Which ions does it contain, and how many of each?

Cytochrome aa3 complex consists almost entirely of alpha helices

Put the following in order A- Cyt c B- CuA centre C- Cyt a D- Cyt a3-CuB centre E - Water •Transfer from cyt a3-CuB centre to oxygen

How many protons are pumped by complex 4, and how many are used to make water (per Oxygen molecule)?

Which conformation of Complex IV has the low affinity for protons?

Protons bind to Complex IV during Conformation B

What happens during conformation B that lowers the Complex IV's affinity for protons?

No question, just look at it and learn it

ATP synthase is found on the inner mitochondrial membrane

ATP synthase is divided into two sections, F1 & F0 Which is peripheral and which is integral?

Name the function of ATP synthase

If ATP is high, and the proton gradient is low, what will Complex V do instead?

Protons flow through F1 to power the ATP synthesis in F0

Name the subunits of F1

Which of the following subunit(s) are the largest in F1?

What is the role of the delta subunit in F1?

The epsilon subunit of F0 connects the gamma to the C subunit

Put the following in order
A- Cyt c
B- CuA centre
C- Cyt a
D- Cyt a3-CuB centre
E - Water
•Transfer from cyt a3-CuB centre to oxygen

ATP synthase is divided into two sections, F1 & F0
Which is peripheral and which is integral?

What order do the three conformations work in?
Assume you start with the Loose formation