Genetics

Pregunta 1 de 32

1

Of the following polymorphisms which are single nucleotide differences that are seen between two genomes?

Selecciona una de las siguientes respuestas posibles:

Restriction fragment length polymorphisms
Short tandem repeats
Variable number tandem repeats
Single nucleotide polymorphisms
Chromosomal translocation

Explicación

Pregunta 2 de 32

1

Which of the following polymorphisms can be detected through Southern blot?

Selecciona una de las siguientes respuestas posibles:

Short tandem repeats
Single nucleotide polymorphisms
Restriction fragment length polymorphisms
Long interspersed nucleotide sequences

Explicación

Pregunta 3 de 32

1

What does it mean, that a locus is homozygous?

Selecciona una de las siguientes respuestas posibles:

many different alleles
two alleles that are the same
two alleles that are different
no alleles
one allele have been deleted

Explicación

Pregunta 4 de 32

1

The following is a description of which method.
Patients DNA was isolated and cut with HaeIII. Product of the restriction digest was resolved by gel electrophoresis and transferred to a membrane. Then the membrane was exposed to hybridization probes, and the resulting band pattern was compared with that seen in another sample of DNA.

Selecciona una de las siguientes respuestas posibles:

RFLP by Southern blotting
VNTR by PCR
SNP by sequencing
STR by PCR
SNP by Western Blot

Explicación

Pregunta 5 de 32

1

FGA, D8S1179, and D2S11 are examples of which of the following?

Selecciona una de las siguientes respuestas posibles:

RFLP
VNTR
STR
SNP

Explicación

Pregunta 6 de 32

1

Which of the following is used as a marker of gender?

Selecciona una de las siguientes respuestas posibles:

D8S1179
Amelogenin
TPOX
PentaD
CD4

Explicación

Pregunta 7 de 32

1

How many loci are considered core loci by the Federal Bureau of Investigation in the Combined DNA Indexing System?

Selecciona una de las siguientes respuestas posibles:

10
12
13
16
20

Explicación

Pregunta 8 de 32

1

When two sources of DNA are compared by STR analysis in a crime scene, how many differences between alleles will determine that the two sources are different?

Selecciona una de las siguientes respuestas posibles:

1
2
4
6
25

Explicación

Pregunta 9 de 32

1

Which of the following polymorphisms can be used to compare DNA samples derived from males but not females?

Selecciona una de las siguientes respuestas posibles:

STR
Y-STR
RFLP
VNTR
SNP

Explicación

Pregunta 10 de 32

1

Which of the following STRs is a Y-STR?

Selecciona una de las siguientes respuestas posibles:

D5S818
vWA
Penta E
DYS385
LPL

Explicación

Pregunta 11 de 32

1

A child has disputed paternity. The child has the following STR haplotype:
TPOX = 7/8; D5S818 = 11/12; D13S317 = 9/10; vWA = 11/12; D21S11 = 27/28.
Alleged Father 1 has the following STR haplotype: TPOX = 6/10; D5S818 = 9/13; D13S317 = 9/14; vWA = 14/16; D21S11 = 30/33.
What can be said of Alleged Father 1?

Selecciona una de las siguientes respuestas posibles:

He is the father of the child
He cannot be excluded as the father
He is excluded as the father.
More STRs need to be analyzed before a conclusion can be made.

Explicación

Pregunta 12 de 32

1

An individual who has two populations of cells that were derived from two different zygotes is called a:

Selecciona una de las siguientes respuestas posibles:

chimera
mosaic
heteroplasmy
mutant

Explicación

Pregunta 13 de 32

1

A woman has been raped by an unknown man. Which of the following laboratory procedures can be used to more accurately analyze residual DNA left by the man in an effort to identify him?

Selecciona una de las siguientes respuestas posibles:

RFLP
STR
Y-STR
VNTR

Explicación

Pregunta 14 de 32

1

DNA found at the scene of the crime was determined to have one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the following?

Selecciona una de las siguientes respuestas posibles:

Female
Male
Impossible to tell from one locus
This locus does not discriminate between males and females

Explicación

Pregunta 15 de 32

1

STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons?

Selecciona una de las siguientes respuestas posibles:

To determine whether the donor and recipient are a match
To determine the likelihood of graft-versus-host disease
To find informative alleles for post-transplantation studies
To add their STR haplotypes to a national database

Explicación

Pregunta 16 de 32

1

STR analysis was performed on a bone marrow donor and recipient pair with the following results. Which loci are informative?

Selecciona una de las siguientes respuestas posibles:

vWA and TH01
TH01 and D13S317
D16S539 and F13A01
vWA and D13S317

Explicación

Pregunta 17 de 32

1

Bone marrow has been transplanted into a recipient. STR analysis was performed on whole blood from the recipient 6 months after the transplant by PCR, followed by capillary gel electrophoresis with fluorescence measurement of amplicons. For the TPOX locus, the donor and recipient do not share alleles. The fluorescence of the TPOX locus for the donor peak was 15693, and the fluorescence of the TPOX locus for the recipient peak was 5329. What is the percentage of recipient cells remaining?

Selecciona una de las siguientes respuestas posibles:

25%
34%
66%
75%

Explicación

Pregunta 18 de 32

1

Two people have what percentage of DNA sequence that differs between them?

Selecciona una de las siguientes respuestas posibles:

1%
5%
10%
25%
15%

Explicación

Pregunta 19 de 32

1

Two middle-age women who had been adopted into different families as infants discover circumstantially that they may have the same biological mother. Which of the following analyses would give the best information toward proving their maternal lineage?

Selecciona una de las siguientes respuestas posibles:

Y-STR
Mitochondrial DNA
RFLP-PCR
SNP haplotypes

Explicación

Pregunta 20 de 32

1

Consider the following STR analysis:
Which of the child alleles are inherited form the father?

Selecciona una de las siguientes respuestas posibles:

D3S1358: 15, vWA: 18, FGA: 24, TH01: 10 , TPOX: 11, CSF1PO: 12, D5S818: 12, D13S317: 9
D3S1358: 4, vWA: 5, FGA: 20, TH01: 11 , TPOX: 10, CSF1PO: 13, D5S818: 3, D13S317: 6
D3S1358: 15, vWA: 17, FGA: 23, TH01: 6 , TPOX: 11, CSF1PO: 12, D5S818: 10, D13S317: 10
D3S1358: 10, vWA: 17, FGA: 24, TH01: 6 , TPOX: 10, CSF1PO: 12, D5S818: 10, D13S317: 9
D3S1358: 15, vWA: 18, FGA: 24, TH01: 10 , TPOX: 11, CSF1PO: 12, D5S818: 12, D13S317: 10

Explicación

Pregunta 21 de 32

1

Basked on the results which of the alleged fathers (AF) is the father?

Selecciona una de las siguientes respuestas posibles:

AF1
AF2
Neither

Explicación

Pregunta 22 de 32

1

A child and an alleged father (AF) share alleles with the following paternity index:
What is the combined paternity index from these three loci?

Selecciona una de las siguientes respuestas posibles:

4.131
4.5
5
3.131

Explicación

Pregunta 23 de 32

1

With 50% prior odds, what is the probability of paternity from these three loci?

Selecciona una de las siguientes respuestas posibles:

80%
76%
90%
60%

Explicación

Pregunta 24 de 32

1

What is a traditional definition of a polymorphism?

Selecciona una de las siguientes respuestas posibles:

The genets variation must be present in at least 1 % of the population.
The genets variation must be present in at least 5 % of the population.
The genets variation that no longer is present in the population.
The genets variation can not be present in majority of the population.

Explicación

Pregunta 25 de 32

1

Which of the following genomic variations is the hardest to detect?

Selecciona una de las siguientes respuestas posibles:

SNP
STRs
VNTRs
RFLPs

Explicación

Pregunta 26 de 32

1

When comparing a generic similarity go two random individuals, what percentage of their genome is identical?

Selecciona una de las siguientes respuestas posibles:

99.5%
95%
90%
100%
80%

Explicación

Pregunta 27 de 32

1

RFLP stands for?

Selecciona una de las siguientes respuestas posibles:

Restriction fragment length polymorphism
Rapidly Fusing length polymorphisms
Rescue factor logical polymorphisms
Redundant fragment length polymorphism

Explicación

Pregunta 28 de 32

1

Closely linked markers are used for:

Selecciona una de las siguientes respuestas posibles:

Accurate mapping of regions associated with phenotypic characteristics
Finding the length of a sequence
Location of the centromere
Defining the promoter
Disabling the binding of transcription factors

Explicación

Pregunta 29 de 32

1

What is a definition of a haplotype?

Selecciona una de las siguientes respuestas posibles:

A block of closely linked SPS on the same chromosome that tend to be inherited together.
A half of a chromosome.
Chromosome with only one functioning allele.
Allele that gives a disfiguring phenotype.

Explicación

Pregunta 30 de 32

1

Mitochondrial DNA is____

Selecciona una de las siguientes respuestas posibles:

circular double stranded
circular single stranded
linear double stranded
linear single stranded

Explicación

Pregunta 31 de 32

1

Genes encoded on the mtDNA include:

Selecciona una de las siguientes respuestas posibles:

22 tRNA genes, 2 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
30 tRNA genes, 2 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
22 tRNA genes, 6 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
22 tRNA genes, 2 ribosomal RNA genes, and 60 genes coding for components of the oxidation-phosphorylation system.

	Creado por mona1nyc hace más de 10 años

DNA Polymorphisms and Human Identification MB ASCP

Pregunta 1 de 32

Of the following polymorphisms which are single nucleotide differences that are seen between two genomes?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 2 de 32

Which of the following polymorphisms can be detected through Southern blot?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 3 de 32

What does it mean, that a locus is homozygous?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 4 de 32

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 5 de 32

FGA, D8S1179, and D2S11 are examples of which of the following?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 6 de 32

Which of the following is used as a marker of gender?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 7 de 32

How many loci are considered core loci by the Federal Bureau of Investigation in the Combined DNA Indexing System?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 8 de 32

When two sources of DNA are compared by STR analysis in a crime scene, how many differences between alleles will determine that the two sources are different?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 9 de 32

Which of the following polymorphisms can be used to compare DNA samples derived from males but not females?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 10 de 32

Which of the following STRs is a Y-STR?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 11 de 32

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 12 de 32

An individual who has two populations of cells that were derived from two different zygotes is called a:

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 13 de 32

A woman has been raped by an unknown man. Which of the following laboratory procedures can be used to more accurately analyze residual DNA left by the man in an effort to identify him?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 14 de 32

DNA found at the scene of the crime was determined to have one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the following?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 15 de 32

STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 16 de 32

STR analysis was performed on a bone marrow donor and recipient pair with the following results. Which loci are informative?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 17 de 32

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 18 de 32

Two people have what percentage of DNA sequence that differs between them?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 19 de 32

Two middle-age women who had been adopted into different families as infants discover circumstantially that they may have the same biological mother. Which of the following analyses would give the best information toward proving their maternal lineage?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 20 de 32

Consider the following STR analysis: Which of the child alleles are inherited form the father?

Selecciona una de las siguientes respuestas posibles:

Explicación

Pregunta 21 de 32

Consider the following STR analysis:
Which of the child alleles are inherited form the father?

A child and an alleged father (AF) share alleles with the following paternity index:
What is the combined paternity index from these three loci?