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Making DNA fragments from mRNA
1. Extract target mRNA molecules from a cell that produces the required protein.
2. Mix the mRNA with free DNA nucleotides and ( reverse transcriptase, a restriction endonuclease, DNA ligase, DNA polymerase ).
3. The mRNA is used as a template for new strands of ( cDNA, tRNA, rRNA, miRNA ) to be synthesised that are complementary to it.