Gen Lec. Notes 11/1 (Chapter 13; eukaryotes)

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General Genetics Flashcards on Gen Lec. Notes 11/1 (Chapter 13; eukaryotes), created by Isabella N on 04/11/2017.
Isabella N
Flashcards by Isabella N, updated more than 1 year ago
Isabella N
Created by Isabella N about 8 years ago
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Question Answer
Transcription happens in the...? Nucleus
DNA protected by... The nucleus
Introns are segments between...? protein coding sequences on DNA strand
Who needs to get out? Introns.
RNA polymerase 3 + 1's job is to...? Transcribe all inform.
RNA polymerase 2's job is to...? transcribe structure genes, what makes up cell, proteins, tissue, etc.
Ribosomes are the ____ of rRNA. Product.
Transcription is making all the mRNA , which will...? Leave for the cytoplasm.
Exons' purpose. To be part of mRNA + encode for amino acids --> protein
Where does RNA polymerase 2 + 3 happen? Nucleoplasm
Where does RNA polymerase 1 happen? Nucleolus
Upstream is above the ___. Its job is...? Promotor; regulate how much mRNA is made + amount of transcription
The promoter has (3) things. TATA box, transcriptional starting point, regulatory elements
Who effects transcription regulation? Promotors + enchanchers
The mRNA starting point is...? A little extra RNA
The TATA box's job is...? To make sure transcription happens @ right place.
There are ___ transcription factors who serve as a ____ point for RNA polymerase. Many; beginning
The core promoter is ___ in length + is the ____ box. It serves as a ____ _____ point for transcription. Short; TATA; precise starting
The core promoter is the site for what to happen? RNA polym.2 can bind to DNA + begin making RNA from it.
What are the control elements? Silencers, enhancers + proximal-promoter elements
What is basal transcription? The small amount of transcription the core promoter does.
What do the control elements do? Help to determine the efficiently/ rate of RNA Polym.2 job's
Enhancers do what? Make transcription of the RNA polym.2 go faster.
Silencers do what? Stop transcription process of RNA polym.2
Proximal-promoters are usually where? Just before the promotor region.
Silencers + enhancers are usually where? A long distance before the promoter region.
Cis-acting elements rule over what? Nearby genes
Cis-acting elements are? Enhancers, silencers, TATA box
Trans-acting elements are what? The control proteins, transciption factors
Who helps RNA polym.2 bind to DNA sequen. + influences how well it does its job Trans-acting elements
General transcription factors do what? The requirement for RNA polym.2 to attach to promoter + start working
HnRNA is made into...? mRNA
How does hnRNA --> mRNA? Capping 5' end, adding poly-A tail to 3' end + taking out introns
How does capping 5' work? Capping enym. adds triphosphate bridge to RNA's last 5'
Why is 5' capped? To stabilize the pre-RNA
The poly-A tail is prevents what? The transcribed RNA from falling apart before going to the cytoplasm.
Exons are ___ _____ after intron are ___ ___. stitched together; cut out
A mature + ready mRNA is made of? Only exons.
Who handles are introns? Spliceosomes
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