Cell Lec. Notes - 11/6 - Genetic Code and Transcription (Eukary.)

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For exam 2
Isabella N
Flashcards by Isabella N, updated more than 1 year ago
Isabella N
Created by Isabella N about 7 years ago
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Eukary. are similar to prokary. transcription-how? They both have (4) steps
Eukar. have _ RNA polymerase. 3
For binding to happen for the RNA polym., what needs to present @ the ____? Transcription factors; promoter
What happens after RNA strand is made and while it is being made? Editing + processing
Which RNA polym. is carried out in the ___? All (3) of them; nucleus
For accuracy to begin initiation, RNA polym. needs these _ sequences? A core promoter + upstream control element
The core promoter contains what? The startpoint
RNA polym. _, needs _ components in the core promoter 2; 4
The DNA sequences found in core promotor elements - in 5' - 3'order (including startpoint) Trans.Fact.2B recognition element ->TATA box ->Initiator (has startpoint) -> downst, prom. element
BRE is short for? Transcription Factor 2B
(2) types of core promoters for RNA polym. _ TATA-driven + Downst.prom.Elem.-driven
TATA-driven promoters must have ___ + ____, but may not have ___. Initiator + TATA box with/without BRE
DPE-driven promoters must have __ + ___ Initiator + DPE
The core promoter w/o help can do what? Transcription a small amount
Increase promotors needs more ____ ____, which does what? Control elements; can do promote more effiecitenly
Proximal promoter elements are how far form startpoint? 100-200 nucleotides
RNA polym. _ uses only what? Down stream promoters
RNA polym. _ uses () types of promoters: ____ + __ ___ 3; 2; tRNA + 5S RNA
tRNA promoters has what? consensus box A + B
5S promoters has what? consensus box A + C
A general transcription factor is known as...? Basal transcription factor
Basal transcrip. factors is what? a protein needs by RNA polym. 2
What's the job of basal transcrip. factor? To help RNa polym. 2 bind to promoter--> initiate transcrip. (no matter the genes present)
What is needed transcribe nuclear genes? General transcription factors
RNA polym. _ makes ____ from what? 1; Ribosomes from ribosomal RNA (rRNA)
RNA polym. _ makes these (2) things and where? 2; small nucleic RINA + per-RNA; in nucleus
RNA polym. _ is responsible for ___ genes. 3; nuclear
Before RNA polym. binds, what needs to be made and bound to first> Transcription complex
How is the transcription complex formed? Transcription factors coming to "stage" @ right time
Where does transcrip. factors bind to ? TATA box-building around core promoter sequence
Which transcription factor "activates" the transcription-giving off ___? TB2H; ATP
RNA polym. _ is terminated by a (__) protein ______ signal sequence 1; 18; nucleotide;
RNA polym. _ is terminated by a ____ sequence of this nucleotide. 3; short; U's
RNA polym. _ is terminated by getting ___ to form a ____ _ ____. Cut; poly A tail
Where is RNA polym. _ terminal sequence? 10-35 nucleotides downstream of A's + U's
New RNA that has just been transcribed are? Primary transcript, pre-rRNA, being made into mature rRNA
How is a rRNA made? Cut spacers from precursor + paste those segments together
Where is rRNA made? in the nucleolus @ nucleolus organizer region
Who helps RNA poly. _ make the rRNA? 1; small nucleolar RNAs
tRNa is made from? the precursor pre-tRNA
While tRNA is being edited, what chemical changes are seen? Methylation, creation
In the ____, mRNA is _____ + in the ____, it's _____. nucleus; transcribed; cytoplasm; translated
Each mRNA codes for (_) ____. 1; Polypeptide
Mature mRNA has a _' cap made of what + where is linked to? 5; methylated guanosine; 5' end of mRNA
The purpose of the 5' cap on the _RNA. m; to stabilize the strand + prevent it from degrading + position properly on ribosome
mRNA has ____ _ ____ connect to 3' end. poly A tail
What is the poly A tail made of? Adenine;
How is the poly A tail added? By the enzy. polyA polymerase
The cell make 100's of copies of _RNA- why? t; make sure there is enough to spare
What is a ribosome made of? Mature rRNA + protein
Where is the poly A tail attached to? 3' end of mRNA after 10-35 nucleotides sequence
The purpose of the poly A tail? 2nd level of protection + mRNA is known by ribosomes + proteins know mRNA needs to leave nucleus
What is taken out of mRNA? Introns
What is found in mature RNA? Exons + a poly A tail + a 5' cap
Who takes out introns? Spliceosomes, enzymes
Who wraps up introns + takes them away while pasting exons together? snRNPs, RNA-protein complexes
How does splicing take place? snRNPs see each of the ends of intron where they must bind to
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