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Description
Flashcards by J yadonknow, updated more than 1 year ago
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Created by J yadonknow
almost 7 years ago
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| Question | Answer |
| What did Louis Pasteur discover? | Disproved spontaneous generation Showed heat could sterilise Showed atmosphere is full of MO |
| What did Robert Koch prove? | That MO caused disease, forming Koch's postulate |
| How did he do this? | 4 postulates 1. Suspect pathogen must be present in all cases of disease, absent in healthy 2. Suspect pathogen must be grow in pure growth culture 3. Cells from pure culture of suspect causes disease in healthy animals. 4. Suspect must be reisolated and look similar to OG sample. |
| What is a slope? | Set agar in a tube, used for pure growth of a MO |
| When do you use no agar tests? | For liquid, used for blood tests |
| What is the problem with Brightfield microscopy? | Kills the MO |
| How can this be avoided? | Using Phase contrast and Darkfield microscopy which keeps sample alive |
| What is DIC microscopy? | Use of polarised light to make cellular structures appear more 3D. |
| What is Atomic Force microscopy? | Measured force between probe and atoms of cell's surface Used to look at pili |
| What is Confocal Scanning Laser microscopy? (CSL) | Use laser with a fluorescent microscope Cells usually stained with fluorescent dyes |
| What is Electron microscopy? | Electrons used instead of visible light Whole system operates in a vacuum |
| What is Transmission Electron microscopy? | TEM is high magnitude and high resolution Requires a thin slice of specimen as electrons can't penetrate tissue |
| What is scanning electron microscopy? | Specimen coated in a heavy metal i.e. Gold and visualised by electron scatter |
| What types of MO exist? (7) | Basteria Archaea (2 phylum) Protozoa Algae Prions Viruses Fungi |
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