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Description
Flashcards by Rebecca Hedworth, updated more than 1 year ago
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Created by Rebecca Hedworth
over 6 years ago
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| Question | Answer |
| Qualitative Analysis | Any method of identifying the chemical components in a sample. |
| Quantitative Analysis | Working out how much of a given chemical component there is in a sample. |
| Chromatography | The separation of the components of a mixture dissolved in a liquid or gas (the mobile phase) carrying it through a structure holding the stationary phase. |
| Adsorbent | Often used to describe the stationary phase in chromatography because substances become adsorbed to it during separation. |
| Adsorption | When a substance (e.g. a gas, liquid or solute) binds to or attaches to another, usually solid. |
| Polar | A polar (hydrophilic) substance will dissolve in or mix with water. |
| Elution | To wash out. In column chromatography this means 'washing out' a substance that has become adsorbed to the column (stationary phase). |
| Eluent | The solvent (mobile phase) used to wash substances out of a column. |
| Eluate | The mobile phase, containing dissolved substances, as it emerges from a column. |
| Gas Chromatography (GC) | A separation technique using an inert carrier gas as the mobile phase and a thin layer of liquid or polymer on an inert solid support as the stationary phase. |
| High Performance Liquid Chromatography (HPLC) | A type of column chromatography that uses very small particles and high pressures to achieve better separation. It can be used for analysis or, on a larger scale, for purification. |
| Gel Electrophoresis | The use of an electric field for separation of compounds based on differences in their charge and size. Usually for DNA. |
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