Fixatives

Description

Histotechnology Flashcards on Fixatives, created by Victoria Station on 25/03/2019.
Victoria Station
Flashcards by Victoria Station, updated more than 1 year ago
Victoria Station
Created by Victoria Station over 5 years ago
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Resource summary

Question Answer
Formaldehyde Non-coagulant (methylene bridges), additive 37-40% formaldehyde gas in water penetrates quick, fixes slowly +NaCl to correct osmolality increase basophilia
10% Neutral Buffered Formalin Non-coagulant (methylene bridges), additive 4% formaldehyde gas in water frozen sections possible increase basophilia *best general fixative
Acetic Acid Non-coagulant, non-additive fixes nuclei lyses red blood cells tissue swelling *best fixative for nuclei
Picric Acid Coagulant, additive destroys nucleic acids removes calcium and iron from tissue increases acidophilic stains tissue yellow also a mordant
Gluteraldehyde Non-coagulant, Additive crosslinking but no methylene bridge
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