Aseptic Technique

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Principles of Science Flashcards on Aseptic Technique, created by sportycanine on 20/01/2014.
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Flashcards by sportycanine, updated more than 1 year ago
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Created by sportycanine almost 11 years ago
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Antisepsis Prevention of sepsis by the destruction or inhibition of microorganisms using an agent that may be applied safely to living tissue
Antiseptic Chemical agent that either kills or inhibits pathogenic microorganisms; this term is reserved for agents applied to the body
Asepsis Absence of pathogenic microbes in living tissues
Disinfectant Germicidal chemical substance that kills microorganisms on inanimate objects, such as items of surgical equipment
Disinfection Removal of microorganisms, but not necessarily their spores
Sepsis Presence of pathogens or their toxic products in the tissues of a patient
Sterilisation Complete elimination of microbial viability, including spores, by physical/chemical means.
What are sources of bacterial contamination? Surgical team, surgical equipment, operating theater environment, and the patient
What are some non-sterile barriers? Scrub suit, surgical head covers, shoes and shoe covers, and face masks
What are the sterile barriers? Surgical gloves and surgical gowns
What are physical methods of equipment sterilisation? Heat, irradiation, and filtration
How can heat be used for surgical equipment sterilisation? Steam: saturated steam under pressure; 13 min at 120 degrees Moist heat (boiling): cannot guarantee to kill all microorganisms and spores, 100 degrees insufficient
What are chemical methods of sterilisation? Ethylene oxide: inactivates cellular DNA; toxic to tissues Hydrogen peroxide gas plasma: UV photons and radicals Cold steralisation: more disinfection, not for instruments used beneath body surface
What is the preoperative preparation of the patient, generally speaking? Removal of hair from the surgical site and adjacent areas, cleansing and use of antiseptic agents, and aspetic draping of the surgical site.
What are the major determinants of surgical wound infection? 1. bacterial factors 2. surgical wound factors: blood supply present 3. host risk factors - systemic and local host defences
Clean (NRC classification) Non-traumatic wound; elective surgery with primary intention healing; no inflammation encountered; no break in aseptic technique; resp, alimentary and urogenital tract not entered
Clean-contaminated (NRC classification) GI or resp tract entered without significant spillage; urogenital tract entered in the absence of infection; biliary tract entered in the absence of infected bile; minor break in aseptic technique
Contaminated (NRC classification) gross spillage from the GI tract; enterance of urogenital or biliary tract in the presence of infection; fresh traumatic wound (<4 hrs); major break in aseptic technique
Dirty (NRC classification) Perforated viscus encountered; acute bacterial inflammation +/- pus encountered; traumatic wound: devitalised tissue, FBs, fecal contamination, >4hrs old; transection of clean tissue to gain access to a collection of pus
Antimicrobial prophylaxis administration of an antimicrobial agent to the patient before the contamination of the surgical site
Antimicrobial drugs commonly used amoxicillin/clavulanate; cefazolin (1st generation cephalosporin); and cefuroxime (2nd generation cephalosporin)
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