Bioprocessing with Immobilised Cells

Description

When microorganisms are used in a bioreactor, they are removed along with the product at the end of the process. To prevent waste of micro-organisms, they are often fized or immobilised in the bioreactor. This flashcard deck outlines the main points and would appeal to anyone studying intermediate level biology.
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Resource summary

Question Answer
Bioprocessing use of enzyme controlled reactions to produce a product
Immobilising Cells bonded to each other, bonded or attached to an insoluble support, or enclosed in a gel or membrane
More Recent Immobilising techniques trapping the cells in a gel
Gel used in Alcohol production yeast cells are mixed with sodium alginate
Immobilising the yeast cells using in production of alcohol Beads of this mixture are soaked in calcium chloride, which causes the alginate to enclose and immobilise the yeast cells.
Molecules able to pass through the gel pores Sucrose
Molecules able to leave the pores of the gel Glucose
Molecule unable to leave the pores of the gel Yeast the pores are too small
Advantage of Immobilisation Gentle process - does not damage the cell
Advantage of immobilisation technique Easy to recover the cells
Products from bioprocessing using immobilised cells artificial antibiotics, fertilisers, steroids
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