607676
Description
Flashcards by enrique.salanic, updated more than 1 year ago
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Created by enrique.salanic
over 10 years ago
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| Question | Answer |
| Alu insertion? | It's 300 bp long and it is a pice of mobile DNA which has the ability to copy and move within genome |
| What are the 3 PCR Steps? | 1) Denaturation 2) Annealing 3) Extension |
| Briefly explain the 3 PCR steps? | 1) Denaturation (90-92 C); two strand of DNA helix 2) Annealing (50-60 C); 2 primers to attach to both sides of DNA (aka DNA template) 3) Extension (70-74 C): Synthesis of complementary strands |
| What are the possible PCR results with a section of cur 16 around the Alu insertion? | 1) chr with insertion have PCR product = 941bp 2) chr without insertion have PCR product = 641 bp 3) Two homozygous, 1 or the other 4) 1 heterozygous have both lengths of product |
| Mutation in introns | If there is a mutation in an intron (non coding region DNA) then it does not cause a change in protein and which would be the opposite in if there is a mutation in the exon |
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