HIV Entry and Integration

Question	Answer
Entry Type	Fusion from Without
Initial Protein-Protein Interation During Entry	CD4 (on host lymph) and Gp120 on HIV virus
Secondary Protein-Protein Interation	Cd4 Co-Receptors and viral proteins cause membrane fusion
Replication- Partial Uncoating	Endosome partially uncoats, but genome is still held inside the protein
Genome Organisation- RNA Conversion	Inside capsule, RNA is converted to ds DNA
Genome Organisation- RNA Conversion (1-RNA Primers)	RNA Primers, using the ss RNA as a template, create a strand of cDNA
Genome Organisation- RNA Conversion (2-5' End)	cDNA creation begins at the 5' end and creates a copy of the LTR region
Genome Organisation- RNA Conversion (3-LTR Copy)	This is moved to the 3' end, and used as a primer to copy the whole genome in ssDNA form
Genome Organisation- RNA Conversion (4-RNAase H)	RNAase H destroys the ssRNA part of the genome, leaving only PP, which is used as a primer for the second strand
Genome Organisation- RNA Conversion (?- Circular Intermediate)	There is a possible circular intermediate whilst the 2nd strand is being made
Genome Organisation- RNA Conversion (5- DNA LTR)	The LTR of the ds DNA is longer than the LTR on the original RNA
Integration- PIC	The dsDNA, still inside the protein coat, is transported to the nucleus as the Pre-Integration Complex
Integration- Integrase	Integrase will integrate the ds DNA pretty much anywhere

Resource summary

	Created by gina_evans0312 over 10 years ago