2) Cool to 35 to
primers bind to
start of each
strand, prevent
rejoining + act as
marker for
polymerase
3) heated to 73
+ thermostable
TAQ
polymerase
replicates
strand
each time
process
repeats,
DNA
doubles
DNA probe
locates section of
DNA, consists of short
lengths of single
stranded DNA with
specific nucleotide
sequence. To dectect-
radioactive/fluorescent
for xray/laser scanner
DNA profiling ( fingerprinting)
1) DNA cut
with restriction
endonucleases
into fragments
2) Fragments
seperated by gel
electrophoresis
due to bands,
smaller fragments
move further
3) Bonds heated to
be single stranded,
transferred onto
nylon and washed
with DNA probes
complementary to
MRS's
4) Repeated
with several
probes for
different
MRS's +
fragments
detected
5) DNA bound
to probe show
up, bands are
fingerprints