2172339
Description
Mind Map by maddiebones, updated more than 1 year ago
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Created by maddiebones
over 9 years ago
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DNA
Quantitation
I
- WHAT: Shows how
much DNA is avail.
for ampn.
- Need to be
conc./dil. b4 ampn.
- Need to be
conc./dil. b4 ampn.
- WHY
- Find optimal amt.
- Comm. amp.
kits have narrow
qty range for
optimization
- Too little DNA
- Allele
dropout
(missing)
- Heterozyg.
assumed as
homozyg.
- Heterozyg.
assumed as
homozyg.
- x amp. of some
loci (small amp.,
large not)
- Partial prof. gen.
- Misinterpreted as
degraded DNA ➙low level
data ➙ x cross threshold
➙ x allele call ➙ x data
- Misinterpreted as
degraded DNA ➙low level
data ➙ x cross threshold
➙ x allele call ➙ x data
- Partial prof. gen.
- Locus imbalance
- Unbalanced amp.
of two alleles @ a
locus
- Stochastic
flux in ratio
of two diff
alleles
- Unbalanced amp.
of two alleles @ a
locus
- Allele
dropout
(missing)
- Too much DNA
- Stutter
- Offscale data (high
rfu); > detection
lim.
- 10% of rfu, 4bp
- Offscale data (high
rfu); > detection
lim.
- Pull-up
- Peak of one color ovrlap
- Same bp; diff col.
- Peak of one color ovrlap
- Split
peak (-A)
- add 3' base tail (poly
A tail); so always
expect +A; no time
to add A, so -A
- Shoulder;
Two sep.
peaks; split
- add 3' base tail (poly
A tail); so always
expect +A; no time
to add A, so -A
- Stutter
- Too little DNA
- Comm. amp.
kits have narrow
qty range for
optimization
- X waste
time/res. to
repeat process
- Some
samples
wholly
consumed,
can't be rep.
- Some
samples
wholly
consumed,
can't be rep.
- Troubleshooting
- Reduce time taken
- Reduce time taken
- Det. suitable
ampn. methods
- Target
specific
types of
DNA
- Human
specific assays
- Crime scene
x pristine,
non-human
DNA
- DAB std.
9.3 - det.
qty. of
hDNA
- Crime scene
x pristine,
non-human
DNA
- Both
autosomal
+ Y assay (BENEFITS)
- In crimes with
XY + XX, DNAxx
>> DNAxy ➙
low/no amp. of
DNAxy ➙ FN
- 1 person's DNA
>> 2P ➙ single
source profile, or
2nd profile too
low to use
- Y-specific assays
target DNAxy rgdless
of DNAxx
- Semen present,
little/no sperm cells in
vag swabs
- Mixture; XX
bled more @
scene
- Saliva
transfer frm
XY ➙ XX
(swabbing
skin of XX)
- Y-Quantifiler as
screening tool: high
no. of assault cases;
screening proc.
lengthy
(presumptive +
confirmatory); male
DNA present ➙ put
through screening
process
- Semen present,
little/no sperm cells in
vag swabs
- In crimes with
XY + XX, DNAxx
>> DNAxy ➙
low/no amp. of
DNAxy ➙ FN
- Human
specific assays
- Target
specific
types of
DNA
- Find optimal amt.
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