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22708861
TECNOLOGIA DE ADN RECOMBINANTE
Description
Mind Map on TECNOLOGIA DE ADN RECOMBINANTE, created by Tomas Molina on 09/05/2020.
Mind Map by
Tomas Molina
, updated more than 1 year ago
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Created by
Tomas Molina
over 4 years ago
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Resource summary
TECNOLOGIA DE ADN RECOMBINANTE
VECTORES
Moleculas transportadoras de AND
PLASMIDOS
pBR322
Genes de resitencia
sitios de resticcion unicos
Primero en ser diseñado
PUC18
inserta fragmentos mas grandes
5 a 10 veces mas copias que pBR322
FAGOS
COSMIDOS
DEBEN TENER
Sitios de restriccion
Facil de recuperar
Marcador de seleccion
Resistencia-Antibioticos
Gen de enzimas
Replicacion independiente
Enzimas de restriccion
Aisladas de Bacterias
Actualmente 200 enzimas diferentes
Degradan acido nucleico
Reconocen secuencias especificas
Premio nobel 1987
Werner Arber, Hamilton Smith y Daniel Nathans
Tipos de Enzimas
Tipo I
Corte en una posicion aleatoria
Tipo II
Corte preciso en la secuencia que reconoce
Extremos Cohesivos
Extremos Romos
Utiliza ADN recombinante
Media attachments
Puc18 (binary/octet-stream)
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