37657885
Description
Mind Map by Nada Mohamed, updated more than 1 year ago
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Created by Nada Mohamed
over 2 years ago
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Microscopy
- To calculate magnification:
- Conversions:
- Convert mm to um by multiplying by 1000
- Convert um to nm by multiplying by 1000
- Convert um to nm by multiplying by 1000
- Convert mm to um by multiplying by 1000
- Calibrating a microscope
- Place the stage micrometer under the
clips on the microscope stage.
- Turn the lowest power
objective lens on the nose piece
- Align the scales on the eyepiece graticule and
stage micrometer so that they are parallel
and there are two points of intersection
- Stage micrometer is 1 cm long and divided into 100
divisions ∴ each division: 1 ÷ 100 = 0.01 cm = 100 µm
- 20 eyepiece graticule divisions = 9 stage micrometer
divisions ∴ 20 eyepiece graticule divisions: 9 × 100 = 900
µm ∴ 1 eyepiece graticule division: 900 ÷ 20 = 18 µm
- Process repeated with the other objective
lenses to find a calibration factor for each lens
- Process repeated with the other objective
lenses to find a calibration factor for each lens
- 20 eyepiece graticule divisions = 9 stage micrometer
divisions ∴ 20 eyepiece graticule divisions: 9 × 100 = 900
µm ∴ 1 eyepiece graticule division: 900 ÷ 20 = 18 µm
- Stage micrometer is 1 cm long and divided into 100
divisions ∴ each division: 1 ÷ 100 = 0.01 cm = 100 µm
- Align the scales on the eyepiece graticule and
stage micrometer so that they are parallel
and there are two points of intersection
- Turn the lowest power
objective lens on the nose piece
- Place the stage micrometer under the
clips on the microscope stage.
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